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- W2140313699 abstract "The all-transglycolytic synthesis of lacto-N-biose I (Galbeta1-3GlcNAc) and 3'-sialyl-lacto-N-biose I (NeuAcalpha2-3Galbeta1-3GlcNAc) was performed. The disaccharide lacto-N-biose I was obtained by use of p-nitrophenyl beta-D-galactopyranoside as the donor, 2-acetamido-2-deoxy-D-glucopyranose as the acceptor and Xanthomonas manihotis beta-D-galactosidase as the catalyst. The reaction was shown to be regiospecific, with a high molar yield (about 55%) with respect to the donor. Lacto-N-biose I obtained by this method was used as the acceptor for a subsequent enzymatic reaction catalyzed by Trypanosoma cruzi trans-sialidase in which 2'-(4-methylumbellyferyl)-alpha-D-N-acetylneuraminic was used as the donor of the N-acetylneuraminil moiety. The reaction generated the product, 3'-sialyl-lacto-N-biose I, regiospecifically and with a molar yield of about 35%." @default.
- W2140313699 created "2016-06-24" @default.
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- W2140313699 date "2000-02-01" @default.
- W2140313699 modified "2023-10-10" @default.
- W2140313699 title "Regiospecific glycosidase-assisted synthesis of lacto-N-biose I (Galβ1-3GlcNAc) and 3′-sialyl-lacto-N-biose I (NeuAcα2-3Galβ1-3GlcNAc)" @default.
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- W2140313699 doi "https://doi.org/10.1046/j.1432-1327.2000.01068.x" @default.
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