Matches in SemOpenAlex for { <https://semopenalex.org/work/W2140337191> ?p ?o ?g. }
- W2140337191 endingPage "536" @default.
- W2140337191 startingPage "528" @default.
- W2140337191 abstract "Aims To construct secondary mutant phage-display library of recombinant single variable domain (VHH) against botulinum neurotoxin E by error-prone PCR. Methods and Results The gene coding for specific VHH derived from the camel immunized with binding domain of botulinum neurotoxin E (BoNT/E) was amplified by error-prone PCR. Several biopanning rounds were used to screen the phage-displaying BoNT/E Hc nanobodies. The final nanobody, SHMR4, with increased affinity recognized BoNT/E toxin with no cross-reactivity with other antigens especially with related BoNT toxins. Conclusions The constructed nanobody could be a suitable candidate for VHH-based biosensor production to detect the Clostridium botulinum type E. Significance and Impact of the Study Diagnosis and treatment of botulinum neurotoxins are important. Generation of high-affinity antibodies based on the construction of secondary libraries using affinity maturation step leads to the development of reagents for precise diagnosis and therapy." @default.
- W2140337191 created "2016-06-24" @default.
- W2140337191 creator A5000568569 @default.
- W2140337191 creator A5014528792 @default.
- W2140337191 creator A5020462534 @default.
- W2140337191 creator A5034482331 @default.
- W2140337191 creator A5057596333 @default.
- W2140337191 date "2014-05-16" @default.
- W2140337191 modified "2023-10-06" @default.
- W2140337191 title "Random mutagenesis of BoNT/E Hc nanobody to construct a secondary phage-display library" @default.
- W2140337191 cites W1530865619 @default.
- W2140337191 cites W1964152815 @default.
- W2140337191 cites W1965508878 @default.
- W2140337191 cites W1973643740 @default.
- W2140337191 cites W1977340060 @default.
- W2140337191 cites W1985731056 @default.
- W2140337191 cites W1988046159 @default.
- W2140337191 cites W1988855265 @default.
- W2140337191 cites W1990566414 @default.
- W2140337191 cites W1992021982 @default.
- W2140337191 cites W1994662493 @default.
- W2140337191 cites W2002009217 @default.
- W2140337191 cites W2005895326 @default.
- W2140337191 cites W2006904100 @default.
- W2140337191 cites W2024902076 @default.
- W2140337191 cites W2027872729 @default.
- W2140337191 cites W2033653592 @default.
- W2140337191 cites W2037136314 @default.
- W2140337191 cites W2037182203 @default.
- W2140337191 cites W2037455921 @default.
- W2140337191 cites W2042548343 @default.
- W2140337191 cites W2044481949 @default.
- W2140337191 cites W2045384297 @default.
- W2140337191 cites W2046319392 @default.
- W2140337191 cites W2064975896 @default.
- W2140337191 cites W2069680771 @default.
- W2140337191 cites W2072067155 @default.
- W2140337191 cites W2072689095 @default.
- W2140337191 cites W2074363834 @default.
- W2140337191 cites W2077507635 @default.
- W2140337191 cites W2085081967 @default.
- W2140337191 cites W2090417367 @default.
- W2140337191 cites W2095017920 @default.
- W2140337191 cites W2113569765 @default.
- W2140337191 cites W2122823722 @default.
- W2140337191 cites W2148388239 @default.
- W2140337191 cites W2166103775 @default.
- W2140337191 doi "https://doi.org/10.1111/jam.12526" @default.
- W2140337191 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24766494" @default.
- W2140337191 hasPublicationYear "2014" @default.
- W2140337191 type Work @default.
- W2140337191 sameAs 2140337191 @default.
- W2140337191 citedByCount "8" @default.
- W2140337191 countsByYear W21403371912015 @default.
- W2140337191 countsByYear W21403371912017 @default.
- W2140337191 countsByYear W21403371912019 @default.
- W2140337191 countsByYear W21403371912021 @default.
- W2140337191 countsByYear W21403371912023 @default.
- W2140337191 crossrefType "journal-article" @default.
- W2140337191 hasAuthorship W2140337191A5000568569 @default.
- W2140337191 hasAuthorship W2140337191A5014528792 @default.
- W2140337191 hasAuthorship W2140337191A5020462534 @default.
- W2140337191 hasAuthorship W2140337191A5034482331 @default.
- W2140337191 hasAuthorship W2140337191A5057596333 @default.
- W2140337191 hasBestOaLocation W21403371911 @default.
- W2140337191 hasConcept C104317684 @default.
- W2140337191 hasConcept C104428545 @default.
- W2140337191 hasConcept C143065580 @default.
- W2140337191 hasConcept C153911025 @default.
- W2140337191 hasConcept C159047783 @default.
- W2140337191 hasConcept C159654299 @default.
- W2140337191 hasConcept C16318435 @default.
- W2140337191 hasConcept C167625842 @default.
- W2140337191 hasConcept C185592680 @default.
- W2140337191 hasConcept C186268636 @default.
- W2140337191 hasConcept C2776471321 @default.
- W2140337191 hasConcept C2777010668 @default.
- W2140337191 hasConcept C2777367657 @default.
- W2140337191 hasConcept C2781377902 @default.
- W2140337191 hasConcept C40767141 @default.
- W2140337191 hasConcept C54355233 @default.
- W2140337191 hasConcept C55493867 @default.
- W2140337191 hasConcept C70721500 @default.
- W2140337191 hasConcept C86803240 @default.
- W2140337191 hasConcept C89423630 @default.
- W2140337191 hasConceptScore W2140337191C104317684 @default.
- W2140337191 hasConceptScore W2140337191C104428545 @default.
- W2140337191 hasConceptScore W2140337191C143065580 @default.
- W2140337191 hasConceptScore W2140337191C153911025 @default.
- W2140337191 hasConceptScore W2140337191C159047783 @default.
- W2140337191 hasConceptScore W2140337191C159654299 @default.
- W2140337191 hasConceptScore W2140337191C16318435 @default.
- W2140337191 hasConceptScore W2140337191C167625842 @default.
- W2140337191 hasConceptScore W2140337191C185592680 @default.
- W2140337191 hasConceptScore W2140337191C186268636 @default.
- W2140337191 hasConceptScore W2140337191C2776471321 @default.
- W2140337191 hasConceptScore W2140337191C2777010668 @default.
- W2140337191 hasConceptScore W2140337191C2777367657 @default.