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• W2140341867 abstract "Abstract Background The larvae of the greater wax moth Galleria mellonella are increasingly used (i) as mini-hosts to study pathogenesis and virulence factors of prominent bacterial and fungal human pathogens, (ii) as a whole-animal high throughput infection system for testing pathogen mutant libraries, and (iii) as a reliable host model to evaluate the efficacy of antibiotics against human pathogens. In order to compensate for the lack of genomic information in Galleria , we subjected the transcriptome of different developmental stages and immune-challenged larvae to next generation sequencing. Results We performed a Galleria transcriptome characterization on the Roche 454-FLX platform combined with traditional Sanger sequencing to obtain a comprehensive transcriptome. To maximize sequence diversity, we pooled RNA extracted from different developmental stages, larval tissues including hemocytes, and from immune-challenged larvae and normalized the cDNA pool. We generated a total of 789,105 pyrosequencing and 12,032 high-quality Sanger EST sequences which clustered into 18,690 contigs with an average length of 1,132 bases. Approximately 40% of the ESTs were significantly similar ( E ≤ e -03 ) to proteins of other insects, of which 45% have a reported function. We identified a large number of genes encoding proteins with established functions in immunity related sensing of microbial signatures and signaling, as well as effector molecules such as antimicrobial peptides and inhibitors of microbial proteinases. In addition, we found genes known as mediators of melanization or contributing to stress responses. Using the transcriptomic data, we identified hemolymph peptides and proteins induced upon immune challenge by 2D-gelelectrophoresis combined with mass spectrometric analysis. Conclusion Here, we have developed extensive transcriptomic resources for Galleria . The data obtained is rich in gene transcripts related to immunity, expanding remarkably our knowledge about immune and stress-inducible genes in Galleria and providing the complete sequences of genes whose primary structure have only partially been characterized using proteomic methods. The generated data provide for the first time access to the genetic architecture of immunity in this model host, allowing us to elucidate the molecular mechanisms underlying pathogen and parasite response and detailed analyses of both its immune responses against human pathogens, and its coevolution with entomopathogens." @default.
• W2140341867 created "2016-06-24" @default.
• W2140341867 creator A5045261928 @default.
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• W2140341867 date "2011-06-11" @default.
• W2140341867 modified "2023-10-09" @default.
• W2140341867 title "A comprehensive transcriptome and immune-gene repertoire of the lepidopteran model host Galleria mellonella" @default.
• W2140341867 cites W1548007311 @default.
• W2140341867 cites W1575753808 @default.
• W2140341867 cites W1593317113 @default.
• W2140341867 cites W1964233209 @default.
• W2140341867 cites W1964516192 @default.
• W2140341867 cites W1966692598 @default.
• W2140341867 cites W1968659980 @default.
• W2140341867 cites W1970468669 @default.
• W2140341867 cites W1970621669 @default.
• W2140341867 cites W1974116356 @default.
• W2140341867 cites W1975501068 @default.
• W2140341867 cites W1978027569 @default.
• W2140341867 cites W1979170461 @default.
• W2140341867 cites W1983277269 @default.
• W2140341867 cites W1983429609 @default.
• W2140341867 cites W1985552760 @default.
• W2140341867 cites W1986490524 @default.
• W2140341867 cites W1993640276 @default.
• W2140341867 cites W1999634146 @default.
• W2140341867 cites W2001799545 @default.
• W2140341867 cites W2004283285 @default.
• W2140341867 cites W2005800004 @default.
• W2140341867 cites W2006500919 @default.
• W2140341867 cites W2006748146 @default.
• W2140341867 cites W2017391093 @default.
• W2140341867 cites W2018077219 @default.
• W2140341867 cites W2022821618 @default.
• W2140341867 cites W2023307888 @default.
• W2140341867 cites W2028323983 @default.
• W2140341867 cites W2029743552 @default.
• W2140341867 cites W2035251782 @default.
• W2140341867 cites W2054361477 @default.
• W2140341867 cites W2069181321 @default.
• W2140341867 cites W2073295801 @default.
• W2140341867 cites W2077021099 @default.
• W2140341867 cites W2081582709 @default.
• W2140341867 cites W2082609307 @default.
• W2140341867 cites W2084753086 @default.
• W2140341867 cites W2085474219 @default.
• W2140341867 cites W2088062055 @default.
• W2140341867 cites W2088240141 @default.
• W2140341867 cites W2091962084 @default.
• W2140341867 cites W2096090737 @default.
• W2140341867 cites W2097512519 @default.
• W2140341867 cites W2097804984 @default.
• W2140341867 cites W2098093769 @default.
• W2140341867 cites W2098285182 @default.
• W2140341867 cites W2102549002 @default.
• W2140341867 cites W2109006420 @default.
• W2140341867 cites W2109310735 @default.
• W2140341867 cites W2116963349 @default.
• W2140341867 cites W2117289514 @default.
• W2140341867 cites W2118708091 @default.
• W2140341867 cites W2125121305 @default.
• W2140341867 cites W2128114769 @default.
• W2140341867 cites W2131296612 @default.
• W2140341867 cites W2134363387 @default.
• W2140341867 cites W2141905703 @default.
• W2140341867 cites W2142166235 @default.
• W2140341867 cites W2146058063 @default.
• W2140341867 cites W2149794468 @default.
• W2140341867 cites W2155691418 @default.
• W2140341867 cites W2162838363 @default.
• W2140341867 cites W2164640870 @default.
• W2140341867 cites W2166583116 @default.
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• W2140341867 doi "https://doi.org/10.1186/1471-2164-12-308" @default.
• W2140341867 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3224240" @default.
• W2140341867 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21663692" @default.
• W2140341867 hasPublicationYear "2011" @default.
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