FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2140389074> ?p ?o ?g. }

• W2140389074 endingPage "588" @default.
• W2140389074 startingPage "578" @default.
• W2140389074 abstract "I describe the mechanism whereby ascorbic acid can hamper test systems involving a peroxide-generating system, peroxidase, and a benzidine-type indicator. In test media such as urines, abnormally high concentrations of ascorbic acid may give rise to false negatives in the determination of analytes such as glucose. Absorbance measurements in solution or reflectance measurements on commercially available paper reagent strips demonstrate either inhibition of visible activity by ascorbic acid or a lag time in the development of oxidized indicator color. The duration of the lag time is proportional to the ascorbic acid concentration, is inversely proportional to the enzyme concentration, and is also affected by concentrations of hydrogen peroxide and o-tolidine indicator. The same results were seen in both citrate buffer pH 5 and phosphate buffer pH 7. Because the complete system (o-tolidine indicator, hydrogen peroxide, and peroxidase) must be present if the ascorbate is to be oxidized rapidly, this indicates that ascorbic acid inhibits color development by re-reducing oxidized indicator as fast as it is formed; the o-tolidine then acts catalytically in oxidizing ascorbic acid. Added Cu2+ and Fe3+, both known to react with ascorbic acid, had measurable but small effects on the system. In contrast, Hg2+ abolished the ascorbic acid-elicited lag time, even when present in near-stoichiometric concentration. Hg2+ showed little inhibitory effect on the activity of either glucose oxidase or peroxidase. Presumably it rapidly oxidizes ascorbic acid to dehydroascorbate. The reaction of mercuric ion with ascorbate was measured by reflectance measurements of paper reagent strips in addition to absorbance measurements of solution assays; equivalent results were obtained. Incorporation of Hg2+ into reagent strips can thus render both strips and solution diagnostic tests insensitive to interfering substances such as ascorbic acid." @default.
• W2140389074 created "2016-06-24" @default.
• W2140389074 creator A5024078554 @default.
• W2140389074 date "1982-04-01" @default.
• W2140389074 modified "2023-09-27" @default.
• W2140389074 title "Interference by ascorbic acid in test systems involving peroxidase. I. Reversible indicators and the effects of copper, iron, and mercury." @default.
• W2140389074 cites W136640200 @default.
• W2140389074 cites W1495361419 @default.
• W2140389074 cites W1515119897 @default.
• W2140389074 cites W1528181398 @default.
• W2140389074 cites W154216494 @default.
• W2140389074 cites W1567091047 @default.
• W2140389074 cites W1762180126 @default.
• W2140389074 cites W1797215313 @default.
• W2140389074 cites W1823911033 @default.
• W2140389074 cites W1906172909 @default.
• W2140389074 cites W1969162776 @default.
• W2140389074 cites W1994289112 @default.
• W2140389074 cites W1997065436 @default.
• W2140389074 cites W2000112260 @default.
• W2140389074 cites W2087160849 @default.
• W2140389074 cites W2125628206 @default.
• W2140389074 cites W2132905138 @default.
• W2140389074 cites W2291116316 @default.
• W2140389074 cites W2314555939 @default.
• W2140389074 cites W2320885899 @default.
• W2140389074 cites W61659505 @default.
• W2140389074 doi "https://doi.org/10.1093/clinchem/28.4.578" @default.
• W2140389074 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7074824" @default.
• W2140389074 hasPublicationYear "1982" @default.
• W2140389074 type Work @default.
• W2140389074 sameAs 2140389074 @default.
• W2140389074 citedByCount "34" @default.
• W2140389074 countsByYear W21403890742014 @default.
• W2140389074 countsByYear W21403890742015 @default.
• W2140389074 countsByYear W21403890742018 @default.
• W2140389074 countsByYear W21403890742019 @default.
• W2140389074 countsByYear W21403890742020 @default.
• W2140389074 countsByYear W21403890742023 @default.
• W2140389074 crossrefType "journal-article" @default.
• W2140389074 hasAuthorship W2140389074A5024078554 @default.
• W2140389074 hasBestOaLocation W21403890741 @default.
• W2140389074 hasConcept C13965031 @default.
• W2140389074 hasConcept C162008176 @default.
• W2140389074 hasConcept C178790620 @default.
• W2140389074 hasConcept C179104552 @default.
• W2140389074 hasConcept C181199279 @default.
• W2140389074 hasConcept C185592680 @default.
• W2140389074 hasConcept C2986274086 @default.
• W2140389074 hasConcept C31903555 @default.
• W2140389074 hasConcept C40875361 @default.
• W2140389074 hasConcept C43617362 @default.
• W2140389074 hasConcept C533411734 @default.
• W2140389074 hasConcept C55493867 @default.
• W2140389074 hasConcept C98015330 @default.
• W2140389074 hasConceptScore W2140389074C13965031 @default.
• W2140389074 hasConceptScore W2140389074C162008176 @default.
• W2140389074 hasConceptScore W2140389074C178790620 @default.
• W2140389074 hasConceptScore W2140389074C179104552 @default.
• W2140389074 hasConceptScore W2140389074C181199279 @default.
• W2140389074 hasConceptScore W2140389074C185592680 @default.
• W2140389074 hasConceptScore W2140389074C2986274086 @default.
• W2140389074 hasConceptScore W2140389074C31903555 @default.
• W2140389074 hasConceptScore W2140389074C40875361 @default.
• W2140389074 hasConceptScore W2140389074C43617362 @default.
• W2140389074 hasConceptScore W2140389074C533411734 @default.
• W2140389074 hasConceptScore W2140389074C55493867 @default.
• W2140389074 hasConceptScore W2140389074C98015330 @default.
• W2140389074 hasIssue "4" @default.
• W2140389074 hasLocation W21403890741 @default.
• W2140389074 hasLocation W21403890742 @default.
• W2140389074 hasOpenAccess W2140389074 @default.
• W2140389074 hasPrimaryLocation W21403890741 @default.
• W2140389074 hasRelatedWork W2039945299 @default.
• W2140389074 hasRelatedWork W2043512403 @default.
• W2140389074 hasRelatedWork W2123060630 @default.
• W2140389074 hasRelatedWork W2374722786 @default.
• W2140389074 hasRelatedWork W2382891823 @default.
• W2140389074 hasRelatedWork W2413435323 @default.
• W2140389074 hasRelatedWork W2623697933 @default.
• W2140389074 hasRelatedWork W284034804 @default.
• W2140389074 hasRelatedWork W2951760521 @default.
• W2140389074 hasRelatedWork W2952256733 @default.
• W2140389074 hasVolume "28" @default.
• W2140389074 isParatext "false" @default.
• W2140389074 isRetracted "false" @default.
• W2140389074 magId "2140389074" @default.
• W2140389074 workType "article" @default.