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• W2140465345 endingPage "2682" @default.
• W2140465345 startingPage "2673" @default.
• W2140465345 abstract "The post-translational attachment of biotin and lipoic acid to specific lysine residues displayed in protruding beta-turns in homologous biotinyl and lipoyl domains of their parent enzymes is catalysed by two different ligases. We have expressed in Escherichia coli a sub-gene encoding the biotinyl domain of E.coli acetyl-CoA carboxylase, and by a series of mutations converted the protein from the target for biotinylation to one for lipoylation, in vivo and in vitro. The biotinylating enzyme, biotinyl protein ligase (BPL), and the lipoylating enzyme, LplA, exhibited major differences in the recognition process. LplA accepted the highly conserved MKM motif that houses the target lysine residue in the biotinyl domain beta-turn, but was responsive to structural cues in the flanking beta-strands. BPL was much less sensitive to changes in these beta-strands, but could not biotinylate a lysine residue placed in the DKA motif characteristic of the lipoyl domain beta-turn. The presence of a further protruding thumb between the beta2 and beta3 strands in the wild-type biotinyl domain, which has no counterpart in the lipoyl domain, is sufficient to prevent aberrant lipoylation in E.coli. The structural basis of this discrimination contrasts with other forms of post-translational modification, where the sequence motif surrounding the target residue can be the principal determinant." @default.
• W2140465345 created "2016-06-24" @default.
• W2140465345 creator A5051034667 @default.
• W2140465345 creator A5060338852 @default.
• W2140465345 date "1999-05-17" @default.
• W2140465345 modified "2023-09-30" @default.
• W2140465345 title "Structure and selectivity in post-translational modification: attaching the biotinyl–lysine and lipoyl–lysine swinging arms in multifunctional enzymes" @default.
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• W2140465345 doi "https://doi.org/10.1093/emboj/18.10.2673" @default.
• W2140465345 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1171349" @default.
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• W2140465345 hasPublicationYear "1999" @default.
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