FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2140594235> ?p ?o ?g. }

• W2140594235 endingPage "120" @default.
• W2140594235 startingPage "115" @default.
• W2140594235 abstract "ABSTRACTS Objectives : Due to the morphological similarity and frequent occurrence of intermediate forms as well as morphological variations of aerial part, the correct identification between Rhei Radix et Rhizoma and Rhei Undulatai Rhizoma is very difficult. To develo p a reliable method for correct identification and improving the quality standards of Rhei Radix et Rhizoma and Rhei Undulatai Rhizoma, we analyzed RAPD and developed SCAR marker.Methods : To amplify target DNA at the genomic level, 32 Operon 10-mer random primers were applied with four Rheum species, R. officinale, R. palmatum, R. tanguticum and R. undulatum. The nucleotide sequences were determined and species-specific prime rs were prepared depending on the species-specific RAPD amplicons after subcloned into the pG EM-Teasy vector. To develop the SCAR markers, species-specific PCR amplification and mutiplex-PCR were carried out using the single species-specific primer pairs and combinations of them, respectively.Results : We used RAPD analysis of four Rheum plant species to obtain several species-specific RAPD amplicons. From nucleotide sequences of these RAPD amplic ons, we developed two SCAR markers that amplified 314 bp and 390 bp DNA fragments in only R. undulatum but not in R. officinale, R. palmatum, R. tanguticum and R. undulatum, for distinguishing Rhei Undulatai Rhizoma and Rhei Radix et Rhizoma. Furthermore, we established SCAR mar kers for the simultaneous discrimination of the three species within a single reaction by using multiplex-PCR.Conclusions : These genetic markers can be used for the efficient discrimina tion of plants species and commercial herbal medicines between Rhei Undulatai Rhizoma and Rhei Radix et Rhizoma, to ultimately prevent indiscriminate distribution and prescription of these herbal medicines.Key words : Rheum species, Rhei Radix et Rhizoma, Rhei Undulatai Rhizoma, Sequenc e Characterized Amplified Region(SCAR) marker, Random Amplified Polymorphic DNA (RAPD), Molecular authentication" @default.
• W2140594235 created "2016-06-24" @default.
• W2140594235 creator A5030685487 @default.
• W2140594235 creator A5033889770 @default.
• W2140594235 creator A5058177669 @default.
• W2140594235 creator A5072252870 @default.
• W2140594235 creator A5077555810 @default.
• W2140594235 creator A5081030872 @default.
• W2140594235 creator A5086860721 @default.
• W2140594235 creator A5091786215 @default.
• W2140594235 date "2009-01-01" @default.
• W2140594235 modified "2023-09-27" @default.
• W2140594235 title "Development of SCAR Markers for the Discrimination of Rhei Radix et Rhizoma and Rhei Undulatai Rhizoma based on the RAPD" @default.
• W2140594235 cites W1982682630 @default.
• W2140594235 cites W2004159241 @default.
• W2140594235 cites W2012927231 @default.
• W2140594235 cites W2036633135 @default.
• W2140594235 cites W2045770689 @default.
• W2140594235 cites W2066627715 @default.
• W2140594235 cites W2074837644 @default.
• W2140594235 cites W2096298665 @default.
• W2140594235 cites W2161863335 @default.
• W2140594235 cites W2171114940 @default.
• W2140594235 cites W2224643170 @default.
• W2140594235 cites W2503466642 @default.
• W2140594235 hasPublicationYear "2009" @default.
• W2140594235 type Work @default.
• W2140594235 sameAs 2140594235 @default.
• W2140594235 citedByCount "1" @default.
• W2140594235 countsByYear W21405942352012 @default.
• W2140594235 crossrefType "journal-article" @default.
• W2140594235 hasAuthorship W2140594235A5030685487 @default.
• W2140594235 hasAuthorship W2140594235A5033889770 @default.
• W2140594235 hasAuthorship W2140594235A5058177669 @default.
• W2140594235 hasAuthorship W2140594235A5072252870 @default.
• W2140594235 hasAuthorship W2140594235A5077555810 @default.
• W2140594235 hasAuthorship W2140594235A5081030872 @default.
• W2140594235 hasAuthorship W2140594235A5086860721 @default.
• W2140594235 hasAuthorship W2140594235A5091786215 @default.
• W2140594235 hasConcept C104317684 @default.
• W2140594235 hasConcept C178790620 @default.
• W2140594235 hasConcept C185592680 @default.
• W2140594235 hasConcept C2777563447 @default.
• W2140594235 hasConcept C2779091547 @default.
• W2140594235 hasConcept C2908647359 @default.
• W2140594235 hasConcept C49105822 @default.
• W2140594235 hasConcept C54355233 @default.
• W2140594235 hasConcept C556039675 @default.
• W2140594235 hasConcept C59822182 @default.
• W2140594235 hasConcept C71924100 @default.
• W2140594235 hasConcept C8185291 @default.
• W2140594235 hasConcept C81977670 @default.
• W2140594235 hasConcept C84623179 @default.
• W2140594235 hasConcept C86803240 @default.
• W2140594235 hasConcept C99454951 @default.
• W2140594235 hasConceptScore W2140594235C104317684 @default.
• W2140594235 hasConceptScore W2140594235C178790620 @default.
• W2140594235 hasConceptScore W2140594235C185592680 @default.
• W2140594235 hasConceptScore W2140594235C2777563447 @default.
• W2140594235 hasConceptScore W2140594235C2779091547 @default.
• W2140594235 hasConceptScore W2140594235C2908647359 @default.
• W2140594235 hasConceptScore W2140594235C49105822 @default.
• W2140594235 hasConceptScore W2140594235C54355233 @default.
• W2140594235 hasConceptScore W2140594235C556039675 @default.
• W2140594235 hasConceptScore W2140594235C59822182 @default.
• W2140594235 hasConceptScore W2140594235C71924100 @default.
• W2140594235 hasConceptScore W2140594235C8185291 @default.
• W2140594235 hasConceptScore W2140594235C81977670 @default.
• W2140594235 hasConceptScore W2140594235C84623179 @default.
• W2140594235 hasConceptScore W2140594235C86803240 @default.
• W2140594235 hasConceptScore W2140594235C99454951 @default.
• W2140594235 hasIssue "4" @default.
• W2140594235 hasLocation W21405942351 @default.
• W2140594235 hasOpenAccess W2140594235 @default.
• W2140594235 hasPrimaryLocation W21405942351 @default.
• W2140594235 hasRelatedWork W1964313767 @default.
• W2140594235 hasRelatedWork W2016849682 @default.
• W2140594235 hasRelatedWork W2028689761 @default.
• W2140594235 hasRelatedWork W2036633135 @default.
• W2140594235 hasRelatedWork W2066627715 @default.
• W2140594235 hasRelatedWork W2081365571 @default.
• W2140594235 hasRelatedWork W2126541181 @default.
• W2140594235 hasRelatedWork W2162893151 @default.
• W2140594235 hasRelatedWork W2317127872 @default.
• W2140594235 hasRelatedWork W2324968190 @default.
• W2140594235 hasRelatedWork W2342538839 @default.
• W2140594235 hasRelatedWork W2347377325 @default.
• W2140594235 hasRelatedWork W2349949935 @default.
• W2140594235 hasRelatedWork W2358399504 @default.
• W2140594235 hasRelatedWork W2382512279 @default.
• W2140594235 hasRelatedWork W2409245765 @default.
• W2140594235 hasRelatedWork W2469135550 @default.
• W2140594235 hasRelatedWork W2745251542 @default.
• W2140594235 hasRelatedWork W2888693415 @default.
• W2140594235 hasRelatedWork W362210363 @default.
• W2140594235 hasVolume "24" @default.
• W2140594235 isParatext "false" @default.
• W2140594235 isRetracted "false" @default.

• next