Matches in SemOpenAlex for { <https://semopenalex.org/work/W2140700237> ?p ?o ?g. }
- W2140700237 endingPage "5473" @default.
- W2140700237 startingPage "5466" @default.
- W2140700237 abstract "ABSTRACT In Trypanosoma brucei , transcription resistant to the mushroom toxin α-amanitin is not restricted to the rRNA genes (rDNA), as in higher eukaryotes, but extends to genes encoding the major cell surface proteins variant surface glycoprotein (VSG) and procyclin or procyclic acidic repetitive protein (PARP). Here, we report the development of a homologous cell extract from procyclic T. brucei cells in which rDNA and PARP A and VSG gene promoters drive efficient, accurate, and α-amanitin-resistant transcription. A comparative analysis revealed that transcription from the three promoters generally required identical reaction conditions for maximal efficiency. Nevertheless, PARP promoter transcription proved to be exceptional by its high efficiency, its lag phase, a high template DNA concentration optimum, and its tolerance to increasing concentrations of Mn 2+ . Mutational analysis for both the PARP and rDNA promoters showed that the proximal and distal core elements were essential for efficient transcription in vitro. Deletion of the upstream control regions (UCRs), however, had a different effect. Whereas PARP UCR deletion reduced transcription efficiency almost 10-fold, deletion of the rDNA UCR had only a minor effect on transcription efficiency." @default.
- W2140700237 created "2016-06-24" @default.
- W2140700237 creator A5003008763 @default.
- W2140700237 creator A5031453339 @default.
- W2140700237 creator A5044396203 @default.
- W2140700237 creator A5069518693 @default.
- W2140700237 date "1999-08-01" @default.
- W2140700237 modified "2023-09-25" @default.
- W2140700237 title "In Vitro Analysis of α-Amanitin-Resistant Transcription from the rRNA, Procyclic Acidic Repetitive Protein, and Variant Surface Glycoprotein Gene Promoters in Trypanosoma brucei" @default.
- W2140700237 cites W104844502 @default.
- W2140700237 cites W1495819488 @default.
- W2140700237 cites W1546300285 @default.
- W2140700237 cites W1558941855 @default.
- W2140700237 cites W1594451352 @default.
- W2140700237 cites W1658375928 @default.
- W2140700237 cites W1793025992 @default.
- W2140700237 cites W180758222 @default.
- W2140700237 cites W1857239892 @default.
- W2140700237 cites W1974533240 @default.
- W2140700237 cites W1976136946 @default.
- W2140700237 cites W1990057412 @default.
- W2140700237 cites W1991594851 @default.
- W2140700237 cites W1996860074 @default.
- W2140700237 cites W2008099529 @default.
- W2140700237 cites W2009818892 @default.
- W2140700237 cites W2023491365 @default.
- W2140700237 cites W2027286811 @default.
- W2140700237 cites W2031620624 @default.
- W2140700237 cites W2040808908 @default.
- W2140700237 cites W2043036622 @default.
- W2140700237 cites W2050489815 @default.
- W2140700237 cites W2056319265 @default.
- W2140700237 cites W2077485996 @default.
- W2140700237 cites W2083514010 @default.
- W2140700237 cites W2085860463 @default.
- W2140700237 cites W2086989416 @default.
- W2140700237 cites W2094936648 @default.
- W2140700237 cites W2103884702 @default.
- W2140700237 cites W2105181541 @default.
- W2140700237 cites W2129264956 @default.
- W2140700237 cites W2136738776 @default.
- W2140700237 cites W2139115053 @default.
- W2140700237 cites W2143381397 @default.
- W2140700237 cites W2165536404 @default.
- W2140700237 cites W2172519691 @default.
- W2140700237 cites W2343248132 @default.
- W2140700237 cites W4236784973 @default.
- W2140700237 cites W4240268207 @default.
- W2140700237 cites W4294216491 @default.
- W2140700237 doi "https://doi.org/10.1128/mcb.19.8.5466" @default.
- W2140700237 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/84388" @default.
- W2140700237 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10409736" @default.
- W2140700237 hasPublicationYear "1999" @default.
- W2140700237 type Work @default.
- W2140700237 sameAs 2140700237 @default.
- W2140700237 citedByCount "59" @default.
- W2140700237 countsByYear W21407002372012 @default.
- W2140700237 countsByYear W21407002372013 @default.
- W2140700237 countsByYear W21407002372014 @default.
- W2140700237 countsByYear W21407002372015 @default.
- W2140700237 countsByYear W21407002372016 @default.
- W2140700237 countsByYear W21407002372017 @default.
- W2140700237 countsByYear W21407002372019 @default.
- W2140700237 countsByYear W21407002372020 @default.
- W2140700237 countsByYear W21407002372021 @default.
- W2140700237 crossrefType "journal-article" @default.
- W2140700237 hasAuthorship W2140700237A5003008763 @default.
- W2140700237 hasAuthorship W2140700237A5031453339 @default.
- W2140700237 hasAuthorship W2140700237A5044396203 @default.
- W2140700237 hasAuthorship W2140700237A5069518693 @default.
- W2140700237 hasBestOaLocation W21407002372 @default.
- W2140700237 hasConcept C101762097 @default.
- W2140700237 hasConcept C104317684 @default.
- W2140700237 hasConcept C138885662 @default.
- W2140700237 hasConcept C150194340 @default.
- W2140700237 hasConcept C153911025 @default.
- W2140700237 hasConcept C179926584 @default.
- W2140700237 hasConcept C2779502636 @default.
- W2140700237 hasConcept C41895202 @default.
- W2140700237 hasConcept C54355233 @default.
- W2140700237 hasConcept C64350747 @default.
- W2140700237 hasConcept C86803240 @default.
- W2140700237 hasConcept C95444343 @default.
- W2140700237 hasConceptScore W2140700237C101762097 @default.
- W2140700237 hasConceptScore W2140700237C104317684 @default.
- W2140700237 hasConceptScore W2140700237C138885662 @default.
- W2140700237 hasConceptScore W2140700237C150194340 @default.
- W2140700237 hasConceptScore W2140700237C153911025 @default.
- W2140700237 hasConceptScore W2140700237C179926584 @default.
- W2140700237 hasConceptScore W2140700237C2779502636 @default.
- W2140700237 hasConceptScore W2140700237C41895202 @default.
- W2140700237 hasConceptScore W2140700237C54355233 @default.
- W2140700237 hasConceptScore W2140700237C64350747 @default.
- W2140700237 hasConceptScore W2140700237C86803240 @default.
- W2140700237 hasConceptScore W2140700237C95444343 @default.
- W2140700237 hasIssue "8" @default.
- W2140700237 hasLocation W21407002371 @default.
- W2140700237 hasLocation W21407002372 @default.