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• W2140817242 endingPage "749" @default.
• W2140817242 startingPage "738" @default.
• W2140817242 abstract "A previously described type virus stock (designated PP-1R), isolated by cocultivating baboon cells with mink cells transformed by Kirsten sarcoma virus (64J1), has been further cloned and characterized. End point-diluted stocks of PP-1R have been obtained that are free of focus-forming activity and lack both Kirsten sarcoma and primate type C viral sequences. Nucleic acid hybridization experiments show that the cloned virus (MiLV) is an endogenous, genetically transmitted virus of the mink (Mustela vison). MiLV replicates in canine, feline, and 64J1 mink cells but not in an untransformed mink cell line. Multiple viral gene copies can be detected in the DNA of normal mink cells in culture and in normal mink tissues; related endogenous viral genes are also detected in several related Mustela species. The virus codes for a p30 protein very closely related antigenically to that of feline leukemia virus but contains p15 and p12 proteins that are antigenically distinct. The mink cell line, Mv1Lu, and its Kirsten sarcoma-transformed derivatives, 64J1, express relatively low levels of type C viral RNA related to MiLV and normally do not produce detectable levels of MiLV p30 protein or complete, infectious viral particles. Infection of sarcoma virus-transformed mink cells with baboon type C virus, however, can augment the level of expression of endogenous mink viral RNA and can result in the synthesis and packaging of mink viral RNA and p30 antigen in extracellular virions. Since the Mv1Lu cell line and its tranformed derivatives have become widely used in studies of retroviruses, the possibility of activating endogenous mink viral genes should be considered by investigators working with these cells." @default.
• W2140817242 created "2016-06-24" @default.
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• W2140817242 creator A5057414955 @default.
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• W2140817242 date "1978-03-01" @default.
• W2140817242 modified "2023-09-26" @default.
• W2140817242 title "Endogenous mink (Mustela vison) type C virus isolated from sarcoma virus-transformed mink cells" @default.
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• W2140817242 doi "https://doi.org/10.1128/jvi.25.3.738-749.1978" @default.
• W2140817242 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/525967" @default.
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