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• W2140829667 abstract "ChIP coupled with next-generation sequencing (ChIP-seq) has revolutionized whole-genome mapping of DNA-binding protein sites. Although ChIP-seq rapidly gained support in eukaryotic systems, it remains underused in the mapping of bacterial transcriptional regulator-binding sites. Using the virulence-required iron-responsive ferric uptake regulator (Fur), we report a simple, broadly applicable ChIP-seq method in the pathogen Vibrio cholerae. Combining our ChIP-seq results with available microarray data, we clarify direct and indirect Fur regulation of known iron-responsive genes. We validate a subset of Fur-binding sites in vivo and show a common motif present in all Fur ChIP-seq peaks that has enhanced binding affinity for purified V. cholerae Fur. Further analysis shows that V. cholerae Fur directly regulates several additional genes associated with Fur-binding sites, expanding the role of this transcription factor into the regulation of ribosome formation, additional transport functions, and unique sRNAs." @default.
• W2140829667 created "2016-06-24" @default.
• W2140829667 creator A5006281221 @default.
• W2140829667 creator A5045253972 @default.
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• W2140829667 date "2011-07-12" @default.
• W2140829667 modified "2023-10-17" @default.
• W2140829667 title "Mapping the regulon of <i>Vibrio cholerae</i> ferric uptake regulator expands its known network of gene regulation" @default.
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• W2140829667 doi "https://doi.org/10.1073/pnas.1107894108" @default.
• W2140829667 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3145737" @default.
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• W2140829667 hasPublicationYear "2011" @default.
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