Matches in SemOpenAlex for { <https://semopenalex.org/work/W2140950564> ?p ?o ?g. }
Showing items 1 to 93 of
93
with 100 items per page.
- W2140950564 endingPage "319" @default.
- W2140950564 startingPage "312" @default.
- W2140950564 abstract "Cellular dielectric spectroscopy (CDS) is an emerging technology capable of detecting a range of whole-cell responses in a label-free manner. A new CDS-based instrument, CellKey, has been developed that is optimized for G-protein coupled receptor (GPCR) detection and has automated liquid handling in microplate format, thereby making CDS accessible to lead generation/optimization drug discovery. In addition to having sufficient throughput, new assay technologies must pass rigorous standards for assay development, signal window, dynamic range, and reproducibility to effectively support drug discovery SAR studies. Here, the authors evaluated CellKey with 3 different G(i)-coupled GPCRs for suitability in supporting SAR studies. Optimized assay conditions compatible with the precision, reproducibility, and throughput required for routine screening were quickly achieved for each target. Across a 1000-fold range in compound potencies, CellKey results correlated with agonist and antagonist data obtained using classical methods ([(35)S]GTPgammaS binding and cAMP production). For partial agonists, relative efficacy measurements also correlated with GTPgammaS data. CellKey detection of positive allosteric modulators appeared superior to GTPgammaS methodology. Agonist and antagonist activity could be accurately quantified under conditions of low receptor expression. CellKey is a new technology platform that uses label-free detection in a homogeneous assay that is unaffected by color quenching and is easily integrated into existing microtiter-based compound testing and data analysis procedures for drug discovery." @default.
- W2140950564 created "2016-06-24" @default.
- W2140950564 creator A5020062559 @default.
- W2140950564 creator A5053545145 @default.
- W2140950564 creator A5058702148 @default.
- W2140950564 creator A5060391268 @default.
- W2140950564 creator A5066586426 @default.
- W2140950564 creator A5071700019 @default.
- W2140950564 creator A5083362664 @default.
- W2140950564 date "2007-04-01" @default.
- W2140950564 modified "2023-10-16" @default.
- W2140950564 title "Evaluation of Cellular Dielectric Spectroscopy, a Whole-Cell, Label-Free Technology for Drug Discovery on Gi-Coupled GPCRs" @default.
- W2140950564 cites W1953204375 @default.
- W2140950564 cites W1963952222 @default.
- W2140950564 cites W1967597978 @default.
- W2140950564 cites W1977486054 @default.
- W2140950564 cites W1982748514 @default.
- W2140950564 cites W1991001144 @default.
- W2140950564 cites W1995537200 @default.
- W2140950564 cites W2003012589 @default.
- W2140950564 cites W2033660427 @default.
- W2140950564 cites W2126980069 @default.
- W2140950564 cites W2140657586 @default.
- W2140950564 doi "https://doi.org/10.1177/1087057106298637" @default.
- W2140950564 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17307886" @default.
- W2140950564 hasPublicationYear "2007" @default.
- W2140950564 type Work @default.
- W2140950564 sameAs 2140950564 @default.
- W2140950564 citedByCount "74" @default.
- W2140950564 countsByYear W21409505642012 @default.
- W2140950564 countsByYear W21409505642013 @default.
- W2140950564 countsByYear W21409505642014 @default.
- W2140950564 countsByYear W21409505642015 @default.
- W2140950564 countsByYear W21409505642016 @default.
- W2140950564 countsByYear W21409505642017 @default.
- W2140950564 countsByYear W21409505642018 @default.
- W2140950564 countsByYear W21409505642019 @default.
- W2140950564 countsByYear W21409505642020 @default.
- W2140950564 countsByYear W21409505642021 @default.
- W2140950564 countsByYear W21409505642023 @default.
- W2140950564 crossrefType "journal-article" @default.
- W2140950564 hasAuthorship W2140950564A5020062559 @default.
- W2140950564 hasAuthorship W2140950564A5053545145 @default.
- W2140950564 hasAuthorship W2140950564A5058702148 @default.
- W2140950564 hasAuthorship W2140950564A5060391268 @default.
- W2140950564 hasAuthorship W2140950564A5066586426 @default.
- W2140950564 hasAuthorship W2140950564A5071700019 @default.
- W2140950564 hasAuthorship W2140950564A5083362664 @default.
- W2140950564 hasBestOaLocation W21409505641 @default.
- W2140950564 hasConcept C135285700 @default.
- W2140950564 hasConcept C170493617 @default.
- W2140950564 hasConcept C185592680 @default.
- W2140950564 hasConcept C2778684643 @default.
- W2140950564 hasConcept C2778938600 @default.
- W2140950564 hasConcept C41008148 @default.
- W2140950564 hasConcept C43617362 @default.
- W2140950564 hasConcept C51323132 @default.
- W2140950564 hasConcept C55493867 @default.
- W2140950564 hasConcept C74187038 @default.
- W2140950564 hasConcept C9893847 @default.
- W2140950564 hasConceptScore W2140950564C135285700 @default.
- W2140950564 hasConceptScore W2140950564C170493617 @default.
- W2140950564 hasConceptScore W2140950564C185592680 @default.
- W2140950564 hasConceptScore W2140950564C2778684643 @default.
- W2140950564 hasConceptScore W2140950564C2778938600 @default.
- W2140950564 hasConceptScore W2140950564C41008148 @default.
- W2140950564 hasConceptScore W2140950564C43617362 @default.
- W2140950564 hasConceptScore W2140950564C51323132 @default.
- W2140950564 hasConceptScore W2140950564C55493867 @default.
- W2140950564 hasConceptScore W2140950564C74187038 @default.
- W2140950564 hasConceptScore W2140950564C9893847 @default.
- W2140950564 hasIssue "3" @default.
- W2140950564 hasLocation W21409505641 @default.
- W2140950564 hasLocation W21409505642 @default.
- W2140950564 hasOpenAccess W2140950564 @default.
- W2140950564 hasPrimaryLocation W21409505641 @default.
- W2140950564 hasRelatedWork W1135792216 @default.
- W2140950564 hasRelatedWork W1475153 @default.
- W2140950564 hasRelatedWork W1865162173 @default.
- W2140950564 hasRelatedWork W2014346822 @default.
- W2140950564 hasRelatedWork W2036002012 @default.
- W2140950564 hasRelatedWork W2086566345 @default.
- W2140950564 hasRelatedWork W2124910405 @default.
- W2140950564 hasRelatedWork W2177786495 @default.
- W2140950564 hasRelatedWork W2551232629 @default.
- W2140950564 hasRelatedWork W2614037532 @default.
- W2140950564 hasVolume "12" @default.
- W2140950564 isParatext "false" @default.
- W2140950564 isRetracted "false" @default.
- W2140950564 magId "2140950564" @default.
- W2140950564 workType "article" @default.