Matches in SemOpenAlex for { <https://semopenalex.org/work/W2140955834> ?p ?o ?g. }
Showing items 1 to 95 of
95
with 100 items per page.
- W2140955834 endingPage "531" @default.
- W2140955834 startingPage "502" @default.
- W2140955834 abstract "Chlorella virus PBCV-1 DNA ligase seals nicked DNA substrates consisting of a 5'-phosphate-terminated strand and a 3'-hydroxyl-terminated strand annealed to a bridging DNA template strand. The enzyme discriminates at the DNA binding step between substrates containing a 5'-phosphate versus a 5'-hydroxyl at the nick. Mutational analysis of the active site motif KxDGxR (residues 27-32) illuminates essential roles for the conserved Lys, Asp and Arg moieties at different steps of the ligase reaction. Mutant K27A is unable to form the covalent ligase-(Lys-straightepsilonN-P)-adenylate intermediate and hence cannot activate a nicked DNA substrate via formation of the DNA-adenylate intermediate. Nonetheless, K27A catalyzes phosphodiester bond formation at a pre-adenylated nick. This shows that the active site lysine is not required for the strand closure reaction. K27A binds to nicked DNA-adenylate, but not to a standard DNA nick. This suggests that occupancy of the AMP binding pocket of DNA ligase is important for nick recognition. Mutant D29A is active in enzyme-adenylate formation and binds readily to nicked DNA, but is inert in DNA-adenylate formation. R32A is unable to catalyze any of the three reactions of the ligation pathway and does not bind to nicked DNA." @default.
- W2140955834 created "2016-06-24" @default.
- W2140955834 creator A5014112082 @default.
- W2140955834 creator A5042494837 @default.
- W2140955834 date "1998-01-01" @default.
- W2140955834 modified "2023-09-23" @default.
- W2140955834 title "Chlorella virus DNA ligase: Nick recognition and mutational analysis" @default.
- W2140955834 cites W1515043048 @default.
- W2140955834 cites W1974476489 @default.
- W2140955834 cites W2019442958 @default.
- W2140955834 cites W2036528385 @default.
- W2140955834 cites W2044690574 @default.
- W2140955834 cites W2047877787 @default.
- W2140955834 cites W2056895084 @default.
- W2140955834 cites W2060913542 @default.
- W2140955834 cites W2074247929 @default.
- W2140955834 cites W2076571824 @default.
- W2140955834 cites W2078509827 @default.
- W2140955834 cites W2091404614 @default.
- W2140955834 cites W2091665633 @default.
- W2140955834 cites W2109263179 @default.
- W2140955834 cites W2111051119 @default.
- W2140955834 cites W2124426699 @default.
- W2140955834 cites W2125735909 @default.
- W2140955834 cites W2128182444 @default.
- W2140955834 cites W2129279325 @default.
- W2140955834 cites W2134831636 @default.
- W2140955834 cites W2155564090 @default.
- W2140955834 cites W2174944132 @default.
- W2140955834 cites W934354270 @default.
- W2140955834 doi "https://doi.org/10.1093/nar/26.2.502" @default.
- W2140955834 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/147278" @default.
- W2140955834 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9421510" @default.
- W2140955834 hasPublicationYear "1998" @default.
- W2140955834 type Work @default.
- W2140955834 sameAs 2140955834 @default.
- W2140955834 citedByCount "23" @default.
- W2140955834 countsByYear W21409558342013 @default.
- W2140955834 countsByYear W21409558342015 @default.
- W2140955834 countsByYear W21409558342016 @default.
- W2140955834 countsByYear W21409558342018 @default.
- W2140955834 countsByYear W21409558342019 @default.
- W2140955834 countsByYear W21409558342020 @default.
- W2140955834 countsByYear W21409558342022 @default.
- W2140955834 crossrefType "journal-article" @default.
- W2140955834 hasAuthorship W2140955834A5014112082 @default.
- W2140955834 hasAuthorship W2140955834A5042494837 @default.
- W2140955834 hasBestOaLocation W21409558341 @default.
- W2140955834 hasConcept C104317684 @default.
- W2140955834 hasConcept C153911025 @default.
- W2140955834 hasConcept C156719811 @default.
- W2140955834 hasConcept C164965036 @default.
- W2140955834 hasConcept C175114707 @default.
- W2140955834 hasConcept C2908738455 @default.
- W2140955834 hasConcept C31254050 @default.
- W2140955834 hasConcept C38441620 @default.
- W2140955834 hasConcept C552990157 @default.
- W2140955834 hasConcept C55493867 @default.
- W2140955834 hasConcept C67705224 @default.
- W2140955834 hasConcept C86803240 @default.
- W2140955834 hasConceptScore W2140955834C104317684 @default.
- W2140955834 hasConceptScore W2140955834C153911025 @default.
- W2140955834 hasConceptScore W2140955834C156719811 @default.
- W2140955834 hasConceptScore W2140955834C164965036 @default.
- W2140955834 hasConceptScore W2140955834C175114707 @default.
- W2140955834 hasConceptScore W2140955834C2908738455 @default.
- W2140955834 hasConceptScore W2140955834C31254050 @default.
- W2140955834 hasConceptScore W2140955834C38441620 @default.
- W2140955834 hasConceptScore W2140955834C552990157 @default.
- W2140955834 hasConceptScore W2140955834C55493867 @default.
- W2140955834 hasConceptScore W2140955834C67705224 @default.
- W2140955834 hasConceptScore W2140955834C86803240 @default.
- W2140955834 hasIssue "2" @default.
- W2140955834 hasLocation W21409558341 @default.
- W2140955834 hasLocation W21409558342 @default.
- W2140955834 hasLocation W21409558343 @default.
- W2140955834 hasOpenAccess W2140955834 @default.
- W2140955834 hasPrimaryLocation W21409558341 @default.
- W2140955834 hasRelatedWork W1529717900 @default.
- W2140955834 hasRelatedWork W1549826631 @default.
- W2140955834 hasRelatedWork W1972082770 @default.
- W2140955834 hasRelatedWork W2090925521 @default.
- W2140955834 hasRelatedWork W2109263179 @default.
- W2140955834 hasRelatedWork W2140955834 @default.
- W2140955834 hasRelatedWork W2155564090 @default.
- W2140955834 hasRelatedWork W2276253102 @default.
- W2140955834 hasRelatedWork W2277143964 @default.
- W2140955834 hasRelatedWork W4297340634 @default.
- W2140955834 hasVolume "26" @default.
- W2140955834 isParatext "false" @default.
- W2140955834 isRetracted "false" @default.
- W2140955834 magId "2140955834" @default.
- W2140955834 workType "article" @default.