FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2141163165> ?p ?o ?g. }

• W2141163165 endingPage "3221" @default.
• W2141163165 startingPage "3209" @default.
• W2141163165 abstract "Abstract Sx1TV2/16C is a mouse embryonic stem (ES) cell line in which one copy of the Sox1 gene, an early neuroectodermal marker, has been targeted with a neomycin (G418) selection cassette. A combination of directed differentiation with retinoic acid and G418 selection results in an enriched neural stem cell population that can be further differentiated into neurons. After 6–7 days post‐plating (D6–7PP) most neurons readily fired tetrodotoxin (TTX)‐sensitive action potentials due to the expression of TTX‐sensitive Na + and tetraethylammonium (TEA)‐sensitive K + channels. Neurons reached their maximal cell capacitance after D6–7PP; however, ion channel expression continued until at least D21PP. The percentage of cells receiving spontaneous synaptic currents (s.s.c.) increased with days in culture until 100% of cells received a synaptic input by D20PP. Spontaneous synaptic currents were reduced in amplitude and frequency by TTX, or upon exposure to a Ca 2+ ‐free, 2.5 m m Mg 2+ saline. S.s.c. of rapid decay time constants were preferentially blocked by the nonNMDA glutamatergic receptor antagonists CNQX or NBQX. Ca 2+ levels within ES cell‐derived neurons increased in response to glutamate receptor agonists l ‐glutamate, AMPA, N ‐methyl‐ d ‐aspartate (NMDA) and kainic acid and to acetylcholine, ATP and dopamine. ES cell‐derived neurons also generated cationic and Cl – ‐selective currents in response to NMDA and glycine or GABA, respectively. It was concluded that ES‐derived neurons fire action potentials, receive excitatory and inhibitory synaptic input and respond to various neurotransmitters in a manner akin to primary central neurons." @default.
• W2141163165 created "2016-06-24" @default.
• W2141163165 creator A5005048300 @default.
• W2141163165 creator A5017353112 @default.
• W2141163165 creator A5029542725 @default.
• W2141163165 creator A5032159010 @default.
• W2141163165 creator A5032681830 @default.
• W2141163165 creator A5037277601 @default.
• W2141163165 creator A5038151784 @default.
• W2141163165 creator A5075513265 @default.
• W2141163165 creator A5076246784 @default.
• W2141163165 creator A5090151741 @default.
• W2141163165 date "2004-12-01" @default.
• W2141163165 modified "2023-10-15" @default.
• W2141163165 title "Electrical and neurotransmitter activity of mature neurons derived from mouse embryonic stem cells by Sox-1 lineage selection and directed differentiation" @default.
• W2141163165 cites W1504875278 @default.
• W2141163165 cites W1849161055 @default.
• W2141163165 cites W1930351692 @default.
• W2141163165 cites W1966569961 @default.
• W2141163165 cites W1972413906 @default.
• W2141163165 cites W1972662556 @default.
• W2141163165 cites W1982555416 @default.
• W2141163165 cites W1983274440 @default.
• W2141163165 cites W1998907585 @default.
• W2141163165 cites W2009927583 @default.
• W2141163165 cites W2010975288 @default.
• W2141163165 cites W2015089233 @default.
• W2141163165 cites W2027553817 @default.
• W2141163165 cites W2034829192 @default.
• W2141163165 cites W2037358308 @default.
• W2141163165 cites W2049212652 @default.
• W2141163165 cites W2075405431 @default.
• W2141163165 cites W2087789903 @default.
• W2141163165 cites W2095042541 @default.
• W2141163165 cites W2097310850 @default.
• W2141163165 cites W2138340289 @default.
• W2141163165 cites W2231237347 @default.
• W2141163165 doi "https://doi.org/10.1111/j.1460-9568.2004.03782.x" @default.
• W2141163165 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15610154" @default.
• W2141163165 hasPublicationYear "2004" @default.
• W2141163165 type Work @default.
• W2141163165 sameAs 2141163165 @default.
• W2141163165 citedByCount "28" @default.
• W2141163165 countsByYear W21411631652012 @default.
• W2141163165 countsByYear W21411631652014 @default.
• W2141163165 countsByYear W21411631652015 @default.
• W2141163165 countsByYear W21411631652018 @default.
• W2141163165 countsByYear W21411631652021 @default.
• W2141163165 crossrefType "journal-article" @default.
• W2141163165 hasAuthorship W2141163165A5005048300 @default.
• W2141163165 hasAuthorship W2141163165A5017353112 @default.
• W2141163165 hasAuthorship W2141163165A5029542725 @default.
• W2141163165 hasAuthorship W2141163165A5032159010 @default.
• W2141163165 hasAuthorship W2141163165A5032681830 @default.
• W2141163165 hasAuthorship W2141163165A5037277601 @default.
• W2141163165 hasAuthorship W2141163165A5038151784 @default.
• W2141163165 hasAuthorship W2141163165A5075513265 @default.
• W2141163165 hasAuthorship W2141163165A5076246784 @default.
• W2141163165 hasAuthorship W2141163165A5090151741 @default.
• W2141163165 hasConcept C112592302 @default.
• W2141163165 hasConcept C144024400 @default.
• W2141163165 hasConcept C149923435 @default.
• W2141163165 hasConcept C160268369 @default.
• W2141163165 hasConcept C169760540 @default.
• W2141163165 hasConcept C170493617 @default.
• W2141163165 hasConcept C17077164 @default.
• W2141163165 hasConcept C185592680 @default.
• W2141163165 hasConcept C2779803574 @default.
• W2141163165 hasConcept C2908647359 @default.
• W2141163165 hasConcept C55493867 @default.
• W2141163165 hasConcept C61174792 @default.
• W2141163165 hasConcept C67018056 @default.
• W2141163165 hasConcept C86803240 @default.
• W2141163165 hasConcept C95444343 @default.
• W2141163165 hasConceptScore W2141163165C112592302 @default.
• W2141163165 hasConceptScore W2141163165C144024400 @default.
• W2141163165 hasConceptScore W2141163165C149923435 @default.
• W2141163165 hasConceptScore W2141163165C160268369 @default.
• W2141163165 hasConceptScore W2141163165C169760540 @default.
• W2141163165 hasConceptScore W2141163165C170493617 @default.
• W2141163165 hasConceptScore W2141163165C17077164 @default.
• W2141163165 hasConceptScore W2141163165C185592680 @default.
• W2141163165 hasConceptScore W2141163165C2779803574 @default.
• W2141163165 hasConceptScore W2141163165C2908647359 @default.
• W2141163165 hasConceptScore W2141163165C55493867 @default.
• W2141163165 hasConceptScore W2141163165C61174792 @default.
• W2141163165 hasConceptScore W2141163165C67018056 @default.
• W2141163165 hasConceptScore W2141163165C86803240 @default.
• W2141163165 hasConceptScore W2141163165C95444343 @default.
• W2141163165 hasIssue "12" @default.
• W2141163165 hasLocation W21411631651 @default.
• W2141163165 hasLocation W21411631652 @default.
• W2141163165 hasOpenAccess W2141163165 @default.
• W2141163165 hasPrimaryLocation W21411631651 @default.
• W2141163165 hasRelatedWork W1502257144 @default.
• W2141163165 hasRelatedWork W1504755411 @default.
• W2141163165 hasRelatedWork W2005467921 @default.
• W2141163165 hasRelatedWork W2016425978 @default.

• next