FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2141171507> ?p ?o ?g. }

• W2141171507 endingPage "481" @default.
• W2141171507 startingPage "467" @default.
• W2141171507 abstract "Blood vessel formation is important for many physiological and pathological processes and is therefore a critical target for drug development. Inhibiting angiogenesis to starve a tumour or promoting 'normalization' of tumour vasculature in order to facilitate delivery of anticancer drugs are both areas of active research. Recapitulation of vessel formation by human cells in vitro allows the investigation of cell-cell and cell-matrix interactions in a controlled environment and is therefore a crucial step in developing HCS (high content screening) and HTS (high throughput screening) assays to search for modulators of blood vessel formation. HUVECs (human umbilical-vein endothelial cells) exemplify primary cells used in angiogenesis assays. However, primary cells have significant limitations that include phenotypic decay and/or senescence by six to eight passages in culture, making stable integration of fluorescent markers and large-scale expansion for HTS problematic. To overcome these limitations for HTS, we developed a novel angiogenic model system that employs stable fluorescent endothelial cell lines based on immortalized HMECs (human microvascular endothelial cell). We then evaluated HMEC cultures, both alone and co-cultured with an EMC (epicardial mesothelial cell) line that contributes vascular smooth muscle cells, to determine the suitability for HTS or HCS.The endothelial and epicardial lines were engineered to express a panel of nuclear- and cytoplasm-localized fluorescent proteins to be mixed and matched to suit particular experimental goals. HMECs retained their angiogenic potential and stably expressed fluorescent proteins for at least 13 passages after transduction. Within 8 h after plating on Matrigel, the cells migrated and coalesced into networks of vessel-like structures. If co-cultured with EMCs, the branches formed cylindrical-shaped structures of HMECs surrounded by EMC derivatives reminiscent of vessels. Network formation measurements revealed responsiveness to media composition and control compounds.HMEC-based lines retain most of the angiogenic features of primary endothelial cells and yet possess long-term stability and ease of culture, making them intriguing candidates for large-scale primary HCS and HTS (of ~10000-1000000 molecules). Furthermore, inclusion of EMCs demonstrates the feasibility of using epicardial-derived cells, which normally contribute to smooth muscle, to model large vessel formation. In summary, the immortalized fluorescent HMEC and EMC lines and straightforward culture conditions will enable assay development for HCS of angiogenesis." @default.
• W2141171507 created "2016-06-24" @default.
• W2141171507 creator A5009190340 @default.
• W2141171507 creator A5011414151 @default.
• W2141171507 creator A5014064472 @default.
• W2141171507 creator A5022796313 @default.
• W2141171507 creator A5023012522 @default.
• W2141171507 creator A5038346978 @default.
• W2141171507 creator A5061808340 @default.
• W2141171507 creator A5062253569 @default.
• W2141171507 creator A5069934756 @default.
• W2141171507 creator A5076147125 @default.
• W2141171507 creator A5088643285 @default.
• W2141171507 date "2011-10-01" @default.
• W2141171507 modified "2023-10-05" @default.
• W2141171507 title "Characterization of a novel angiogenic model based on stable, fluorescently labelled endothelial cell lines amenable to scale-up for high content screening" @default.
• W2141171507 cites W1783580437 @default.
• W2141171507 cites W1864853792 @default.
• W2141171507 cites W1964187194 @default.
• W2141171507 cites W1967628309 @default.
• W2141171507 cites W1968325292 @default.
• W2141171507 cites W1973772757 @default.
• W2141171507 cites W1976747060 @default.
• W2141171507 cites W1980564598 @default.
• W2141171507 cites W1982977272 @default.
• W2141171507 cites W1987263161 @default.
• W2141171507 cites W1991001144 @default.
• W2141171507 cites W1995827857 @default.
• W2141171507 cites W1997798902 @default.
• W2141171507 cites W2005100448 @default.
• W2141171507 cites W2005817040 @default.
• W2141171507 cites W2006202974 @default.
• W2141171507 cites W2015809080 @default.
• W2141171507 cites W2030697707 @default.
• W2141171507 cites W2034825883 @default.
• W2141171507 cites W2046473105 @default.
• W2141171507 cites W2051672717 @default.
• W2141171507 cites W2053382117 @default.
• W2141171507 cites W2057147067 @default.
• W2141171507 cites W2062859168 @default.
• W2141171507 cites W2066345054 @default.
• W2141171507 cites W2071262913 @default.
• W2141171507 cites W2103198327 @default.
• W2141171507 cites W2115894894 @default.
• W2141171507 cites W2118799617 @default.
• W2141171507 cites W2130797290 @default.
• W2141171507 cites W2135322304 @default.
• W2141171507 cites W2136879631 @default.
• W2141171507 cites W2140088548 @default.
• W2141171507 cites W2144026946 @default.
• W2141171507 cites W2153331460 @default.
• W2141171507 doi "https://doi.org/10.1042/bc20100146" @default.
• W2141171507 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3538155" @default.
• W2141171507 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21732911" @default.
• W2141171507 hasPublicationYear "2011" @default.
• W2141171507 type Work @default.
• W2141171507 sameAs 2141171507 @default.
• W2141171507 citedByCount "15" @default.
• W2141171507 countsByYear W21411715072012 @default.
• W2141171507 countsByYear W21411715072013 @default.
• W2141171507 countsByYear W21411715072014 @default.
• W2141171507 countsByYear W21411715072015 @default.
• W2141171507 countsByYear W21411715072016 @default.
• W2141171507 countsByYear W21411715072018 @default.
• W2141171507 countsByYear W21411715072019 @default.
• W2141171507 countsByYear W21411715072021 @default.
• W2141171507 countsByYear W21411715072022 @default.
• W2141171507 countsByYear W21411715072023 @default.
• W2141171507 crossrefType "journal-article" @default.
• W2141171507 hasAuthorship W2141171507A5009190340 @default.
• W2141171507 hasAuthorship W2141171507A5011414151 @default.
• W2141171507 hasAuthorship W2141171507A5014064472 @default.
• W2141171507 hasAuthorship W2141171507A5022796313 @default.
• W2141171507 hasAuthorship W2141171507A5023012522 @default.
• W2141171507 hasAuthorship W2141171507A5038346978 @default.
• W2141171507 hasAuthorship W2141171507A5061808340 @default.
• W2141171507 hasAuthorship W2141171507A5062253569 @default.
• W2141171507 hasAuthorship W2141171507A5069934756 @default.
• W2141171507 hasAuthorship W2141171507A5076147125 @default.
• W2141171507 hasAuthorship W2141171507A5088643285 @default.
• W2141171507 hasBestOaLocation W21411715072 @default.
• W2141171507 hasConcept C123012128 @default.
• W2141171507 hasConcept C1491633281 @default.
• W2141171507 hasConcept C157044486 @default.
• W2141171507 hasConcept C190062978 @default.
• W2141171507 hasConcept C202751555 @default.
• W2141171507 hasConcept C2777411675 @default.
• W2141171507 hasConcept C2778608917 @default.
• W2141171507 hasConcept C2780394083 @default.
• W2141171507 hasConcept C502942594 @default.
• W2141171507 hasConcept C54355233 @default.
• W2141171507 hasConcept C55493867 @default.
• W2141171507 hasConcept C81885089 @default.
• W2141171507 hasConcept C86803240 @default.
• W2141171507 hasConcept C95444343 @default.
• W2141171507 hasConceptScore W2141171507C123012128 @default.
• W2141171507 hasConceptScore W2141171507C1491633281 @default.
• W2141171507 hasConceptScore W2141171507C157044486 @default.

• next