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• W2141201649 startingPage "1135" @default.
• W2141201649 abstract "The light microscope has long been used to document the localization of fluorescent molecules in cell biology research. With advances in digital cameras and the discovery and development of genetically encoded fluorophores, there has been a huge increase in the use of fluorescence microscopy to quantify spatial and temporal measurements of fluorescent molecules in biological specimens. Whether simply comparing the relative intensities of two fluorescent specimens, or using advanced techniques like Förster resonance energy transfer (FRET) or fluorescence recovery after photobleaching (FRAP), quantitation of fluorescence requires a thorough understanding of the limitations of and proper use of the different components of the imaging system. Here, I focus on the parameters of digital image acquisition that affect the accuracy and precision of quantitative fluorescence microscopy measurements." @default.
• W2141201649 created "2016-06-24" @default.
• W2141201649 creator A5087324164 @default.
• W2141201649 date "2009-06-29" @default.
• W2141201649 modified "2023-10-18" @default.
• W2141201649 title "Accuracy and precision in quantitative fluorescence microscopy" @default.
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• W2141201649 doi "https://doi.org/10.1083/jcb.200903097" @default.
• W2141201649 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2712964" @default.
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• W2141201649 hasPublicationYear "2009" @default.
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