Matches in SemOpenAlex for { <https://semopenalex.org/work/W2141206612> ?p ?o ?g. }
- W2141206612 endingPage "1136" @default.
- W2141206612 startingPage "1121" @default.
- W2141206612 abstract "Nuclear transcription of Trypanosoma brucei displays unusual features. Most protein-coding genes are organized in large directional gene clusters, which are transcribed polycistronically by RNA polymerase II (pol II) with subsequent processing to generate mature mRNA. Here, we describe the identification and characterization of two trypanosome homologues of transcription elongation factor TFIIS (TbTFIIS1 and TbTFIIS2-1). TFIIS has been shown to aid transcription elongation by relieving arrested pol II. Our phylogenetic analysis demonstrated the existence of four independent TFIIS expansions across eukaryotes. While TbTFIIS1 contains only the canonical domains II and III, the N-terminus of TbTFIIS2-1 contains a PWWP domain and a domain I. TbTFIIS1 and TbTFIIS2-1 are expressed in procyclic and bloodstream form cells and localize to the nucleus in similar, but distinct, punctate patterns throughout the cell cycle. Neither TFIIS protein was enriched in the major pol II sites of spliced-leader RNA transcription. Single RNA interference (RNAi)-mediated knock-down and knockout showed that neither protein is essential. Double knock-down, however, impaired growth. Repetitive failure to generate a double knockout of TbTFIIS1 and TbTFIIS2-1 strongly suggests synthetical lethality and thus an essential function shared by the two proteins in trypanosome growth." @default.
- W2141206612 created "2016-06-24" @default.
- W2141206612 creator A5027929813 @default.
- W2141206612 creator A5030350487 @default.
- W2141206612 creator A5047764521 @default.
- W2141206612 creator A5049792085 @default.
- W2141206612 creator A5056732854 @default.
- W2141206612 creator A5085124376 @default.
- W2141206612 creator A5090215590 @default.
- W2141206612 date "2008-08-05" @default.
- W2141206612 modified "2023-10-18" @default.
- W2141206612 title "Identification and characterization of two trypanosome TFIIS proteins exhibiting particular domain architectures and differential nuclear localizations" @default.
- W2141206612 cites W128151508 @default.
- W2141206612 cites W1531934178 @default.
- W2141206612 cites W1577770943 @default.
- W2141206612 cites W1596462402 @default.
- W2141206612 cites W1963645323 @default.
- W2141206612 cites W1965188894 @default.
- W2141206612 cites W1973767731 @default.
- W2141206612 cites W1973862916 @default.
- W2141206612 cites W1979470231 @default.
- W2141206612 cites W1983811433 @default.
- W2141206612 cites W1986431188 @default.
- W2141206612 cites W1990031610 @default.
- W2141206612 cites W1993551142 @default.
- W2141206612 cites W1994342611 @default.
- W2141206612 cites W1996255730 @default.
- W2141206612 cites W2004617021 @default.
- W2141206612 cites W2014802525 @default.
- W2141206612 cites W2015567936 @default.
- W2141206612 cites W2017251757 @default.
- W2141206612 cites W2022936085 @default.
- W2141206612 cites W2026382372 @default.
- W2141206612 cites W2026574531 @default.
- W2141206612 cites W2027479769 @default.
- W2141206612 cites W2036970927 @default.
- W2141206612 cites W2042188385 @default.
- W2141206612 cites W2044793296 @default.
- W2141206612 cites W2059466542 @default.
- W2141206612 cites W2062630554 @default.
- W2141206612 cites W2063000913 @default.
- W2141206612 cites W2064198724 @default.
- W2141206612 cites W2067953727 @default.
- W2141206612 cites W2083387716 @default.
- W2141206612 cites W2088037026 @default.
- W2141206612 cites W2088834030 @default.
- W2141206612 cites W2090690907 @default.
- W2141206612 cites W2091168900 @default.
- W2141206612 cites W2092215837 @default.
- W2141206612 cites W2093484456 @default.
- W2141206612 cites W2096275987 @default.
- W2141206612 cites W2096985831 @default.
- W2141206612 cites W2098999612 @default.
- W2141206612 cites W2099889296 @default.
- W2141206612 cites W2103546861 @default.
- W2141206612 cites W2106882534 @default.
- W2141206612 cites W2111565312 @default.
- W2141206612 cites W2112711937 @default.
- W2141206612 cites W2116350092 @default.
- W2141206612 cites W2117791601 @default.
- W2141206612 cites W2121846158 @default.
- W2141206612 cites W2127774996 @default.
- W2141206612 cites W2128071342 @default.
- W2141206612 cites W2142528445 @default.
- W2141206612 cites W2142583257 @default.
- W2141206612 cites W2146058063 @default.
- W2141206612 cites W2147667109 @default.
- W2141206612 cites W2148229351 @default.
- W2141206612 cites W2148980809 @default.
- W2141206612 cites W2150215006 @default.
- W2141206612 cites W2151831732 @default.
- W2141206612 cites W2153023888 @default.
- W2141206612 cites W2154077605 @default.
- W2141206612 cites W2157236463 @default.
- W2141206612 cites W2158364267 @default.
- W2141206612 cites W2158714788 @default.
- W2141206612 cites W2167756186 @default.
- W2141206612 cites W2168591783 @default.
- W2141206612 cites W2168799765 @default.
- W2141206612 cites W2171372104 @default.
- W2141206612 cites W2801944549 @default.
- W2141206612 cites W4255583186 @default.
- W2141206612 doi "https://doi.org/10.1111/j.1365-2958.2008.06348.x" @default.
- W2141206612 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2610381" @default.
- W2141206612 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18627464" @default.
- W2141206612 hasPublicationYear "2008" @default.
- W2141206612 type Work @default.
- W2141206612 sameAs 2141206612 @default.
- W2141206612 citedByCount "6" @default.
- W2141206612 countsByYear W21412066122015 @default.
- W2141206612 countsByYear W21412066122017 @default.
- W2141206612 countsByYear W21412066122019 @default.
- W2141206612 countsByYear W21412066122021 @default.
- W2141206612 crossrefType "journal-article" @default.
- W2141206612 hasAuthorship W2141206612A5027929813 @default.
- W2141206612 hasAuthorship W2141206612A5030350487 @default.
- W2141206612 hasAuthorship W2141206612A5047764521 @default.
- W2141206612 hasAuthorship W2141206612A5049792085 @default.