FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2141231285> ?p ?o ?g. }

• W2141231285 endingPage "63" @default.
• W2141231285 startingPage "51" @default.
• W2141231285 abstract "A panel of monoclonal antibodies (mAbs) was developed to identify polypeptides sorted in subtypes of brain coated vesicles (CVs) and to separate these by immunoprecipitation. The corresponding antigen of some of the mAbs elicited by CV components was present also in synaptosomal plasma membrane, synaptic vesicles, or microsomes. On immunoblots the mAbs reacted with constitutive brain CV proteins, with cargo molecules, and with a novel CV component that interacts with the actin cytoskeleton. Analysis of radioiodinated brain CVs immunoprecipitated with a tubulin antibody revealed that all brain CVs contained tubulin. The mAb A-7C11 recognized a 40-kilodalton (kDa) polypeptide on the clathrin coat and immunoprecipitated one-quarter of the total brain CVs. The mAb S-11D9 reacted with a 44-kDa antigen and immunoprecipitated 25% of the CVs. This antigen (44 kDa) was present in synaptic vesicles and synaptosomal membrane as well. Moreover, this mAb (S-11D9) reacted with a polypeptide of 56 kDa detected only in synaptosomal membrane. A mAb (C-10B2) that reacted with one of the clathrin light chains (LCb) immunoprecipitated 90% of the brain CVs. One of the mAbs immunoprecipitated a CV subtype that displayed a reversed ratio of the clathrin LCs (LCa greater than LCb). Each of the mAbs yielded different immunofluorescent staining patterns of vesicles in culture cell types that included nerve growth factor-differentiated PC12 cells, neuroblastoma cells, and Madin Darby bovine kidney cells. The data suggest that in brain tissue there is a heterogeneous population of CVs with different polypeptide compositions and subcellular distributions and that each of these subtypes performs a different role in nerve cells." @default.
• W2141231285 created "2016-06-24" @default.
• W2141231285 creator A5045136479 @default.
• W2141231285 creator A5059696040 @default.
• W2141231285 creator A5086897962 @default.
• W2141231285 creator A5090396293 @default.
• W2141231285 date "1989-07-01" @default.
• W2141231285 modified "2023-10-16" @default.
• W2141231285 title "Clathrin-Coated Vesicle Subtypes in Mammalian Brain Tissue: Detection of Polypeptide Heterogeneity by Immunoprecipitation with Monoclonal Antibodies" @default.
• W2141231285 cites W1606423922 @default.
• W2141231285 cites W1834395454 @default.
• W2141231285 cites W1901817159 @default.
• W2141231285 cites W1965932448 @default.
• W2141231285 cites W1970587959 @default.
• W2141231285 cites W1971614761 @default.
• W2141231285 cites W1982998571 @default.
• W2141231285 cites W1988930233 @default.
• W2141231285 cites W1989929086 @default.
• W2141231285 cites W1990713759 @default.
• W2141231285 cites W1995793066 @default.
• W2141231285 cites W2002775774 @default.
• W2141231285 cites W2003022694 @default.
• W2141231285 cites W2005535155 @default.
• W2141231285 cites W2005993715 @default.
• W2141231285 cites W2007209577 @default.
• W2141231285 cites W2011744526 @default.
• W2141231285 cites W2015610211 @default.
• W2141231285 cites W2019644150 @default.
• W2141231285 cites W2022022192 @default.
• W2141231285 cites W2038378128 @default.
• W2141231285 cites W2058150917 @default.
• W2141231285 cites W2069512529 @default.
• W2141231285 cites W2073389407 @default.
• W2141231285 cites W2073788464 @default.
• W2141231285 cites W2087354339 @default.
• W2141231285 cites W2089002965 @default.
• W2141231285 cites W2093274325 @default.
• W2141231285 cites W2097604677 @default.
• W2141231285 cites W2100837269 @default.
• W2141231285 cites W2101107912 @default.
• W2141231285 cites W2110067044 @default.
• W2141231285 cites W2116598220 @default.
• W2141231285 cites W2134644439 @default.
• W2141231285 cites W2144107809 @default.
• W2141231285 cites W2153823739 @default.
• W2141231285 cites W2166769784 @default.
• W2141231285 cites W2170954646 @default.
• W2141231285 cites W2172095199 @default.
• W2141231285 cites W4232722496 @default.
• W2141231285 doi "https://doi.org/10.1111/j.1471-4159.1989.tb07294.x" @default.
• W2141231285 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2656917" @default.
• W2141231285 hasPublicationYear "1989" @default.
• W2141231285 type Work @default.
• W2141231285 sameAs 2141231285 @default.
• W2141231285 citedByCount "18" @default.
• W2141231285 crossrefType "journal-article" @default.
• W2141231285 hasAuthorship W2141231285A5045136479 @default.
• W2141231285 hasAuthorship W2141231285A5059696040 @default.
• W2141231285 hasAuthorship W2141231285A5086897962 @default.
• W2141231285 hasAuthorship W2141231285A5090396293 @default.
• W2141231285 hasConcept C130316041 @default.
• W2141231285 hasConcept C147483822 @default.
• W2141231285 hasConcept C148785051 @default.
• W2141231285 hasConcept C153911025 @default.
• W2141231285 hasConcept C159654299 @default.
• W2141231285 hasConcept C203014093 @default.
• W2141231285 hasConcept C2778236600 @default.
• W2141231285 hasConcept C41625074 @default.
• W2141231285 hasConcept C542903549 @default.
• W2141231285 hasConcept C55493867 @default.
• W2141231285 hasConcept C63162447 @default.
• W2141231285 hasConcept C71829478 @default.
• W2141231285 hasConcept C86803240 @default.
• W2141231285 hasConceptScore W2141231285C130316041 @default.
• W2141231285 hasConceptScore W2141231285C147483822 @default.
• W2141231285 hasConceptScore W2141231285C148785051 @default.
• W2141231285 hasConceptScore W2141231285C153911025 @default.
• W2141231285 hasConceptScore W2141231285C159654299 @default.
• W2141231285 hasConceptScore W2141231285C203014093 @default.
• W2141231285 hasConceptScore W2141231285C2778236600 @default.
• W2141231285 hasConceptScore W2141231285C41625074 @default.
• W2141231285 hasConceptScore W2141231285C542903549 @default.
• W2141231285 hasConceptScore W2141231285C55493867 @default.
• W2141231285 hasConceptScore W2141231285C63162447 @default.
• W2141231285 hasConceptScore W2141231285C71829478 @default.
• W2141231285 hasConceptScore W2141231285C86803240 @default.
• W2141231285 hasIssue "1" @default.
• W2141231285 hasLocation W21412312851 @default.
• W2141231285 hasLocation W21412312852 @default.
• W2141231285 hasOpenAccess W2141231285 @default.
• W2141231285 hasPrimaryLocation W21412312851 @default.
• W2141231285 hasRelatedWork W1990562233 @default.
• W2141231285 hasRelatedWork W199672269 @default.
• W2141231285 hasRelatedWork W2032690248 @default.
• W2141231285 hasRelatedWork W2037509311 @default.
• W2141231285 hasRelatedWork W2053986287 @default.
• W2141231285 hasRelatedWork W2064089409 @default.
• W2141231285 hasRelatedWork W2073271257 @default.

• next