FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2141306565> ?p ?o ?g. }

• W2141306565 endingPage "171" @default.
• W2141306565 startingPage "164" @default.
• W2141306565 abstract "ATP-dependent potassium (KATP) channels of neurons are closed in the presence of physiological levels of intracellular ATP and open when ATP is depleted during hypoxia or metabolic damage. The present study investigates hypoxic alterations of purine and pyrimidine nucleotide levels supposed to intracellularly modulate KATP channels. In addition, the effects of the KATP channel activator diazoxide and its antagonist tolbutamide were investigated on ATP, GTP, CTP and UTP levels in slices of the parietal cortex. Hypoxia was evoked by saturation of the medium with 95% N2–5% CO2 instead of 95% O2–5% CO2 for 5 min. Nucleotide contents were measured by anion-exchange HPLC in neutralized perchloric acid extracts obtained from slices frozen immediately at the end of incubation. Hypoxia per se decreased purine and pyrimidine nucleoside triphosphate contents. Thus, ATP and GTP contents were reduced to 69.9 and 77.6% of the respective normoxic levels. UTP and CTP contents were even more decreased (to 60.9 and 41.6%), probably because the salvage pathway of these pyrimidine nucleotides is less effective than that of the purine nucleotides ATP and GTP. While tolbutamide (300 μM) had no effect on the hypoxia-induced decrease of nucleotides, diazoxide at 300, but not 30 μM aggravated the decline of ATP, UTP and CTP to 51.8, 37.5 and 28.5% of the contents observed at normoxia; GTP levels also showed a tendency to decrease after diazoxide application. Tolbutamide (300 μM) antagonized the effects of diazoxide (300 μM). Nucleoside diphosphate (ADP, GDP and UDP) levels were uniformly increased by hypoxia. There was no hypoxia-induced increase of ADP contents in the presence of tolbutamide (300 μM). The ATP/ADP, GTP/GDP and UTP/UDP ratios uniformly declined at a low pO2. However, only the ATP/ADP ratio was decreased further by diazoxide (300 μM). The observed alterations in nucleotide contents may be of importance for long- and short-term processes related to acute cerebral hypoxia. Thus, hypoxia-induced alterations of purine and pyrimidine nucleotide levels may influence the open state of KATP-channels during the period of reversible hypoxic cerebral injury. Furthermore, alterations during the irreversible period of cerebral injury may also arise, as a consequence of decreased pyrimidine nucleotide contents affecting cell survival via protein and DNA synthesis." @default.
• W2141306565 created "2016-06-24" @default.
• W2141306565 creator A5020407206 @default.
• W2141306565 creator A5028318519 @default.
• W2141306565 creator A5048282307 @default.
• W2141306565 creator A5091334077 @default.
• W2141306565 date "1998-11-01" @default.
• W2141306565 modified "2023-09-26" @default.
• W2141306565 title "Tolbutamide attenuates diazoxide-induced aggravation of hypoxic cell injury" @default.
• W2141306565 cites W114074157 @default.
• W2141306565 cites W1530529896 @default.
• W2141306565 cites W1963907349 @default.
• W2141306565 cites W1977634755 @default.
• W2141306565 cites W1979253405 @default.
• W2141306565 cites W1982324844 @default.
• W2141306565 cites W1982892406 @default.
• W2141306565 cites W1983407126 @default.
• W2141306565 cites W1984861949 @default.
• W2141306565 cites W1997720513 @default.
• W2141306565 cites W1997865252 @default.
• W2141306565 cites W2000416877 @default.
• W2141306565 cites W2019099519 @default.
• W2141306565 cites W2022757484 @default.
• W2141306565 cites W2024773521 @default.
• W2141306565 cites W2026661192 @default.
• W2141306565 cites W2029375255 @default.
• W2141306565 cites W2033657502 @default.
• W2141306565 cites W2035326923 @default.
• W2141306565 cites W2038475735 @default.
• W2141306565 cites W2039213160 @default.
• W2141306565 cites W2047603988 @default.
• W2141306565 cites W2053840206 @default.
• W2141306565 cites W2055389039 @default.
• W2141306565 cites W2068118359 @default.
• W2141306565 cites W2072210164 @default.
• W2141306565 cites W2072747383 @default.
• W2141306565 cites W2086201954 @default.
• W2141306565 cites W2087345484 @default.
• W2141306565 cites W2089076032 @default.
• W2141306565 cites W2092283978 @default.
• W2141306565 cites W2099925190 @default.
• W2141306565 cites W2112650290 @default.
• W2141306565 cites W2136545895 @default.
• W2141306565 cites W2142696114 @default.
• W2141306565 cites W2173108077 @default.
• W2141306565 doi "https://doi.org/10.1016/s0006-8993(98)01001-4" @default.
• W2141306565 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9988561" @default.
• W2141306565 hasPublicationYear "1998" @default.
• W2141306565 type Work @default.
• W2141306565 sameAs 2141306565 @default.
• W2141306565 citedByCount "6" @default.
• W2141306565 countsByYear W21413065652020 @default.
• W2141306565 crossrefType "journal-article" @default.
• W2141306565 hasAuthorship W2141306565A5020407206 @default.
• W2141306565 hasAuthorship W2141306565A5028318519 @default.
• W2141306565 hasAuthorship W2141306565A5048282307 @default.
• W2141306565 hasAuthorship W2141306565A5091334077 @default.
• W2141306565 hasConcept C104317684 @default.
• W2141306565 hasConcept C118716 @default.
• W2141306565 hasConcept C126322002 @default.
• W2141306565 hasConcept C134018914 @default.
• W2141306565 hasConcept C181199279 @default.
• W2141306565 hasConcept C185592680 @default.
• W2141306565 hasConcept C2776054734 @default.
• W2141306565 hasConcept C2776476023 @default.
• W2141306565 hasConcept C2776543447 @default.
• W2141306565 hasConcept C2779306644 @default.
• W2141306565 hasConcept C2779564974 @default.
• W2141306565 hasConcept C2779872217 @default.
• W2141306565 hasConcept C512185932 @default.
• W2141306565 hasConcept C55493867 @default.
• W2141306565 hasConcept C71924100 @default.
• W2141306565 hasConcept C86803240 @default.
• W2141306565 hasConceptScore W2141306565C104317684 @default.
• W2141306565 hasConceptScore W2141306565C118716 @default.
• W2141306565 hasConceptScore W2141306565C126322002 @default.
• W2141306565 hasConceptScore W2141306565C134018914 @default.
• W2141306565 hasConceptScore W2141306565C181199279 @default.
• W2141306565 hasConceptScore W2141306565C185592680 @default.
• W2141306565 hasConceptScore W2141306565C2776054734 @default.
• W2141306565 hasConceptScore W2141306565C2776476023 @default.
• W2141306565 hasConceptScore W2141306565C2776543447 @default.
• W2141306565 hasConceptScore W2141306565C2779306644 @default.
• W2141306565 hasConceptScore W2141306565C2779564974 @default.
• W2141306565 hasConceptScore W2141306565C2779872217 @default.
• W2141306565 hasConceptScore W2141306565C512185932 @default.
• W2141306565 hasConceptScore W2141306565C55493867 @default.
• W2141306565 hasConceptScore W2141306565C71924100 @default.
• W2141306565 hasConceptScore W2141306565C86803240 @default.
• W2141306565 hasIssue "1-2" @default.
• W2141306565 hasLocation W21413065651 @default.
• W2141306565 hasLocation W21413065652 @default.
• W2141306565 hasOpenAccess W2141306565 @default.
• W2141306565 hasPrimaryLocation W21413065651 @default.
• W2141306565 hasRelatedWork W109470073 @default.
• W2141306565 hasRelatedWork W1593205053 @default.
• W2141306565 hasRelatedWork W2001846815 @default.
• W2141306565 hasRelatedWork W2016534516 @default.

• next