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• W2141363485 abstract "Extracellular pathogenesis-related proteins, including glucanases, are expressed at cold temperatures in winter rye (Secale cereale) and display antifreeze activity. We have characterized recombinant cold-induced glucanases from winter rye to further examine their roles and contributions to cold tolerance. Both basic beta-1,3-glucanases and an acidic beta-1,3;1,4-glucanase were expressed in Escherichia coli, purified, and assayed for their hydrolytic and antifreeze activities in vitro. All were found to be cold active and to retain partial hydrolytic activity at subzero temperatures (e.g. 14%-35% at -4 degrees C). The two types of glucanases had antifreeze activity as measured by their ability to modify the growth of ice crystals. Structural models for the winter rye beta-1,3-glucanases were developed on which putative ice-binding surfaces (IBSs) were identified. Residues on the putative IBSs were charge conserved for each of the expressed glucanases, with the exception of one beta-1,3-glucanase recovered from nonacclimated winter rye in which a charged amino acid was present on the putative IBS. This protein also had a reduced antifreeze activity relative to the other expressed glucanases. These results support the hypothesis that winter rye glucanases have evolved to inhibit the formation of large, potentially fatal ice crystals, in addition to having enzymatic activity with a potential role in resisting infection by psychrophilic pathogens. Glucanases of winter rye provide an interesting example of protein evolution and adaptation aimed to combat cold and freezing conditions." @default.
• W2141363485 created "2016-06-24" @default.
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• W2141363485 creator A5061660599 @default.
• W2141363485 date "2006-06-30" @default.
• W2141363485 modified "2023-10-06" @default.
• W2141363485 title "Cold-Active Winter Rye Glucanases with Ice-Binding Capacity" @default.
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• W2141363485 doi "https://doi.org/10.1104/pp.106.081935" @default.
• W2141363485 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1533947" @default.
• W2141363485 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16815958" @default.
• W2141363485 hasPublicationYear "2006" @default.
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