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• W2141448408 abstract "Background: Pathological evaluation of sentinel lymph nodes of breast cancer patients is a critical factor in determining the treatment strategy including patients’ outcome. Multiple factors have been known to correlate with disease free survival and clinical outcomes such as sentinel lymph node metastases. Recently, real-time RT-PCR which is a very useful, rapid, high sensitivity and specificity tool has been reported to detect micrometastases in sentinel lymph nodes which could be helpful to identify high risk patients. Objectives: The purpose of this study are: To evaluate the sensitivity, specificity, and accuracy of CK19 detection by multiplex real-time RT-PCR and to elucidate the correlation of multiplex real-time RT-PCR results and clinicopathological parameters in sentinel lymph nodes of Thai breast cancer patients. Material and methods: 119 patients diagnosed as breast cancer stage I or II at Siriraj Hospital during October 2008 – June 2010 were enrolled in this study. RNAs were extracted from their sentinel lymph nodes and cDNAs were synthesized. Multiplex real-time RT-PCR for detection of CK19 was performed and analysed. Patients’ characteristics and pathological parameters were compared with multiplex real-time RT-PCR results. Results: Significant correlation between angiolymphatic and perineural invasion status and CK19 expression was demonstrated. The overall concordance of CK19 gene with pathological results was 72.3%. Sensitivity and specificity for CK19 detected by multiplex real-time RT-PCR were 78.3%, and 66.1%, respectively. Conclusion: Significant correlation between angiolymphatic and perineural invasion status and CK19 expression in sentinel lymph nodes of breast cancer patients was found in this study." @default.
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• W2141448408 date "2012-01-01" @default.
• W2141448408 modified "2023-10-02" @default.
• W2141448408 title "CK19 Detection by Multiplex Real-Time RT-PCR Correlates with the Presence of Angiolymphatic and Perineural Invasion in Sentinel Lymph Nodes of Thai Breast Cancer Patients" @default.
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• W2141448408 doi "https://doi.org/10.4172/2161-0681.1000122" @default.
• W2141448408 hasPublicationYear "2012" @default.
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