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- W2141506670 abstract "In vivo gene transfer by lipofection was studied in the mouse lung to develop a gene therapy protocol for disorders in which the lung is affected, such as cystic fibrosis. The bacterial lacZ gene encoding beta-galactosidase was used as a reporter, and the X-GAL staining procedure was optimized for cryostat sections of mouse lung. Three to five days after intratracheal instillation of a lacZ DNA-liposome mixture, lacZ expression was shown in a high percentage of airway epithelium cells. The staining proved to be restricted mainly to the epithelium of the bronchi." @default.
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- W2141506670 date "1993-01-01" @default.
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- W2141506670 title "In Vivo Transfer and Expression of the lacZ Gene in the Mouse Lung" @default.
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- W2141506670 doi "https://doi.org/10.3109/01902149309031719" @default.
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