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- W2141608643 abstract "The action of amphotericin B on the K+ permeability of Mycoplasma mycoides var. capri cells, containing either cholesterol or ergosterol in their membranes, was studied. When the drug, solubilized in dimethyl sulfoxide, was added directly to the cell suspension, a slightly greater sensitivity to permeabilization was observed for ergosterol-containing cells, confirming the data reported in the literature. When amphotericin B bound to gel state phospholipid vesicles was added to the cell suspension, two effects on cholesterol-containing cells were observed. First, the K+ active transport rates increased; membrane permeabilization and K+ leakage were subsequently detected. For ergosterol-containing cells these sequential events were observed only at amphotericin B concentrations below 10(-6) M. At higher concentrations only K+ leakage was observed. The second permeabilization effect varied with the amphotericin B concentration in different ways in the two types of cells. The permeabilization of ergosterol-containing membranes depended on the amphotericin B/phospholipid molar ratio, whereas the permeabilization of cholesterol-containing membranes did not. In general, the latter remained fairly constant when the total amphotericin B concentration in the medium varied." @default.
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- W2141608643 date "1985-08-01" @default.
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- W2141608643 title "Transfer of amphotericin B from gel state vesicles to mycoplasma cells: biphasic action on potassium transport and permeability" @default.
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- W2141608643 doi "https://doi.org/10.1128/aac.28.2.167" @default.
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