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- W2141633227 abstract "Ornithobacterium rhinotracheale (ORT) is a respiratory pathogen which has been isolated throughout theworld from numerous bird species. The present study was designed to isolate and characterize the ORTfrom domestic turkeys, quails and pigeons. For this purpose, 250 samples from each bird species (turkey,quail and pigeon) with or without respiratory signs were tested by taking of tracheal swabs. In addition,respiratory tissue samples (tracheal and lung), from 250 slaughtered turkeys, 50 slaughtered quails and 100dead pigeons were also subjected to culture for ORT as tracheal swabs. Respiratory tissues were also testedfor bacterial DNA by using polymerase chain reaction (PCR). In general, 30 isolates including 4 isolatesfrom turkeys, 3 isolates from quails and 23 isolates from pigeons were identified as ORT by bacteriologicalmethod and then confirmed by PCR. Bacterial DNA was detected in 20%, 50% and 35% of respiratorytissues in turkeys, quails and pigeons respectively. Five ORT isolates from pigeon and all four isolates fromturkey showed smaller colony size, while other isolates had larger colonies when cultured in blood agar.Fifty percent of the isolates with larger colony but none of the isolates with small colony size couldagglutinate red blood cells (RBCs). All of the isolates were sensitive to danofloxacin and chloramphenicolwhile more than 90% of pigeon isolates were resistant to ampicillin. All of turkey and quail and 30% ofpigeon isolates were resistant to tetracycline. Our ORT isolates showed high identity (98%- 100%) insequence of 16S rRNA gene to related data in GeneBank." @default.
- W2141633227 created "2016-06-24" @default.
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- W2141633227 date "2011-11-01" @default.
- W2141633227 modified "2023-10-05" @default.
- W2141633227 title "Isolation and characterization of Ornithobacterium rhinotracheale in the commercial turkey, quail flocks and domestic pigeons by bacteriological and molecular methods" @default.
- W2141633227 doi "https://doi.org/10.22092/ari.2016.103875" @default.
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