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• W2141641107 abstract "The authors used a homogeneous calcium dye kit with a cell line transfected using a recombinant protein construct to screen a 50,000 compound library for G-protein coupled receptor (GPCR) agonists. Only 1 of the 365 primary hits activated Gq-coupled GPCRs, as shown using IP-ONE HTRF. Furthermore, an agonist screen against the entire compound library and same heterologous cell line using AequoScreen technology generated no false positives and identified the same positive hit. Next, a multiplex assay composed of both Fluo-3 and Fura-2-loaded cells identified 1 false positive and the same true-positive hit out of the 365 primary hits. Finally, rescreening the 365 primary hits against the parental cell line loaded using the homogeneous calcium dye kit confirmed the specificity of the same true-positive hit only. In summary, the results suggest that AequoScreen technology, IP-ONE HTRF, and multiplex assays are unique, orthogonal technologies to identify nonspecific hits." @default.
• W2141641107 created "2016-06-24" @default.
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• W2141641107 date "2007-03-01" @default.
• W2141641107 modified "2023-10-06" @default.
• W2141641107 title "Identifying Nonselective Hits from a Homogeneous Calcium Assay Screen" @default.
• W2141641107 cites W1990397785 @default.
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• W2141641107 doi "https://doi.org/10.1177/1087057106298538" @default.
• W2141641107 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17289936" @default.
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