FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2141648177> ?p ?o ?g. }

• W2141648177 endingPage "3779" @default.
• W2141648177 startingPage "3772" @default.
• W2141648177 abstract "Asymmetric rolling circle replication of the promiscuous replicon pMV158 is initiated by the plasmid-encoded RepB protein. In vitro, purified RepB protein introduces a nick within the leading strand origin of replication by a nucleophylic attack on the phosphodiester bond at the dinucleotide GpA. Some changes within and around this dinucleotide were recognized by the protein. RepB nicked and closed supercoiled pMV158 DNA, having an optimum activity at 60°C. We have imitated, in vitro, a process of rolling circle replication, since RepB was able to nick (initiation) and to covalently close (termination) single-stranded oligonucleotides containing the protein cleavage sequence. Covalent DNA-protein complexes were not found, indicating that RepB has unique features among plasmid-encoded proteins involved in rolling-circle replication or conjugative mobilization. Asymmetric rolling circle replication of the promiscuous replicon pMV158 is initiated by the plasmid-encoded RepB protein. In vitro, purified RepB protein introduces a nick within the leading strand origin of replication by a nucleophylic attack on the phosphodiester bond at the dinucleotide GpA. Some changes within and around this dinucleotide were recognized by the protein. RepB nicked and closed supercoiled pMV158 DNA, having an optimum activity at 60°C. We have imitated, in vitro, a process of rolling circle replication, since RepB was able to nick (initiation) and to covalently close (termination) single-stranded oligonucleotides containing the protein cleavage sequence. Covalent DNA-protein complexes were not found, indicating that RepB has unique features among plasmid-encoded proteins involved in rolling-circle replication or conjugative mobilization." @default.
• W2141648177 created "2016-06-24" @default.
• W2141648177 creator A5001922918 @default.
• W2141648177 creator A5083699538 @default.
• W2141648177 creator A5091199034 @default.
• W2141648177 date "1995-02-01" @default.
• W2141648177 modified "2023-10-10" @default.
• W2141648177 title "Specific Nicking-Closing Activity of the Initiator of Replication Protein RepB of Plasmid pMV158 on Supercoiled or Single-stranded DNA" @default.
• W2141648177 cites W1482717646 @default.
• W2141648177 cites W1486592714 @default.
• W2141648177 cites W1493618122 @default.
• W2141648177 cites W1518022335 @default.
• W2141648177 cites W1560827533 @default.
• W2141648177 cites W1602055538 @default.
• W2141648177 cites W1641219603 @default.
• W2141648177 cites W1830415406 @default.
• W2141648177 cites W1965429865 @default.
• W2141648177 cites W1967527813 @default.
• W2141648177 cites W1988708705 @default.
• W2141648177 cites W1994485364 @default.
• W2141648177 cites W2018508068 @default.
• W2141648177 cites W2030199372 @default.
• W2141648177 cites W2032265783 @default.
• W2141648177 cites W2041233190 @default.
• W2141648177 cites W2046090919 @default.
• W2141648177 cites W2050078498 @default.
• W2141648177 cites W2054743426 @default.
• W2141648177 cites W2066356605 @default.
• W2141648177 cites W2069610427 @default.
• W2141648177 cites W2072371310 @default.
• W2141648177 cites W2072705379 @default.
• W2141648177 cites W2075657255 @default.
• W2141648177 cites W2080668718 @default.
• W2141648177 cites W2080765578 @default.
• W2141648177 cites W2109263179 @default.
• W2141648177 cites W2116902870 @default.
• W2141648177 cites W2117581823 @default.
• W2141648177 cites W2137695121 @default.
• W2141648177 cites W2160940055 @default.
• W2141648177 cites W275666727 @default.
• W2141648177 cites W8662462 @default.
• W2141648177 doi "https://doi.org/10.1074/jbc.270.8.3772" @default.
• W2141648177 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7876118" @default.
• W2141648177 hasPublicationYear "1995" @default.
• W2141648177 type Work @default.
• W2141648177 sameAs 2141648177 @default.
• W2141648177 citedByCount "38" @default.
• W2141648177 countsByYear W21416481772013 @default.
• W2141648177 countsByYear W21416481772014 @default.
• W2141648177 countsByYear W21416481772015 @default.
• W2141648177 countsByYear W21416481772016 @default.
• W2141648177 countsByYear W21416481772017 @default.
• W2141648177 countsByYear W21416481772018 @default.
• W2141648177 countsByYear W21416481772021 @default.
• W2141648177 countsByYear W21416481772022 @default.
• W2141648177 countsByYear W21416481772023 @default.
• W2141648177 crossrefType "journal-article" @default.
• W2141648177 hasAuthorship W2141648177A5001922918 @default.
• W2141648177 hasAuthorship W2141648177A5083699538 @default.
• W2141648177 hasAuthorship W2141648177A5091199034 @default.
• W2141648177 hasBestOaLocation W21416481771 @default.
• W2141648177 hasConcept C104317684 @default.
• W2141648177 hasConcept C129312508 @default.
• W2141648177 hasConcept C151730666 @default.
• W2141648177 hasConcept C153911025 @default.
• W2141648177 hasConcept C158456008 @default.
• W2141648177 hasConcept C175156509 @default.
• W2141648177 hasConcept C178790620 @default.
• W2141648177 hasConcept C180577832 @default.
• W2141648177 hasConcept C18103114 @default.
• W2141648177 hasConcept C185592680 @default.
• W2141648177 hasConcept C22744801 @default.
• W2141648177 hasConcept C38441620 @default.
• W2141648177 hasConcept C43369102 @default.
• W2141648177 hasConcept C54355233 @default.
• W2141648177 hasConcept C552990157 @default.
• W2141648177 hasConcept C67705224 @default.
• W2141648177 hasConcept C73573662 @default.
• W2141648177 hasConcept C7452308 @default.
• W2141648177 hasConcept C86803240 @default.
• W2141648177 hasConceptScore W2141648177C104317684 @default.
• W2141648177 hasConceptScore W2141648177C129312508 @default.
• W2141648177 hasConceptScore W2141648177C151730666 @default.
• W2141648177 hasConceptScore W2141648177C153911025 @default.
• W2141648177 hasConceptScore W2141648177C158456008 @default.
• W2141648177 hasConceptScore W2141648177C175156509 @default.
• W2141648177 hasConceptScore W2141648177C178790620 @default.
• W2141648177 hasConceptScore W2141648177C180577832 @default.
• W2141648177 hasConceptScore W2141648177C18103114 @default.
• W2141648177 hasConceptScore W2141648177C185592680 @default.
• W2141648177 hasConceptScore W2141648177C22744801 @default.
• W2141648177 hasConceptScore W2141648177C38441620 @default.
• W2141648177 hasConceptScore W2141648177C43369102 @default.
• W2141648177 hasConceptScore W2141648177C54355233 @default.
• W2141648177 hasConceptScore W2141648177C552990157 @default.
• W2141648177 hasConceptScore W2141648177C67705224 @default.
• W2141648177 hasConceptScore W2141648177C73573662 @default.
• W2141648177 hasConceptScore W2141648177C7452308 @default.

• next