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- W2141669602 startingPage "441" @default.
- W2141669602 abstract "ABSTRACT Genes required for mating by a and α cells of Saccharomyces cerevisiae (STE, sterile, genes) encode products such as peptide pheromones, pheromone receptors, and proteins responsible for pheromone processing. a-specific STE genes are those required for mating by a cells but not by α cells. To identify new a-specific STE genes, we have employed a novel strategy that enabled us to determine if a ste mutant defective in mating as a is also defective in mating as α without the need to do crosses. This technique involved a strain (K12-14b) of genotype mat a 1 HML? HMR? sir3ts, which mates as a at 25° and as α at 34°. We screened over 40,000 mutagenized colonies derived from K12-14b and obtained 28 a-specific ste mutants. These strains contained mutations in three known a-specific genes- STE2, STE6 and STE14-and in a new gene, STE16. ste16 mutants are defective in the production of the pheromone, a-factor, and exhibit slow growth. Based on the distribution of a-specific ste mutants described here, we infer that we have identified most if not all nonessential genes that can give rise to a-specific mating defects." @default.
- W2141669602 created "2016-06-24" @default.
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- W2141669602 date "1987-03-01" @default.
- W2141669602 modified "2023-09-26" @default.
- W2141669602 title "<b> <i>STE16</i>, a New Gene Required for Pheromone Production by</b> a <b>Cells of <i>Saccharomyces cerevisiae</i> </b>" @default.
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- W2141669602 doi "https://doi.org/10.1093/genetics/115.3.441" @default.
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