FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2141681955> ?p ?o ?g. }

• W2141681955 endingPage "254" @default.
• W2141681955 startingPage "245" @default.
• W2141681955 abstract "In the present report we studied the safety, efficacy and efficiency of using an infrared laser to facilitate IVF by assessing fertilization, development and birth rates after laser-zona drilling (LZD) in 30 subfertile genetically modified (GM) mouse lines. We determined that LZD increased the fertilization rate four to ten times that of regular IVF, thus facilitating the derivation of 26 of 30 (86.7%) GM mouse lines. Cryopreserved two-cell stage embryos derived by LZD-assisted IVF were recovered and developed to blastocysts in vitro at the same rate as frozen–thawed embryos derived by regular IVF. Surprisingly after surgical transfer to pseudopregnant recipients the birth rate of embryos derived by LZD-assisted IVF was significantly lower than that of embryos derived by regular IVF. However this result could be completely mitigated by the addition of 0.25 M sucrose to the culture medium during LZD which caused the oocyte to shrink in volume relative to the perivitelline space. By increasing the distance from the laser target site on the zona pellucida, we hypothesize that the hyperosmotic effect of sucrose reduced the potential for laser-induced cytotoxic thermal damage to the underlying oocytes. With appropriate preparation and cautious application, our results indicate that LZD-assisted IVF is a safe, efficacious and efficient assisted reproductive technology for deriving mutant mouse lines with male factor infertility and subfertility caused by sperm–zona penetration defects." @default.
• W2141681955 created "2016-06-24" @default.
• W2141681955 creator A5013897171 @default.
• W2141681955 creator A5024804141 @default.
• W2141681955 creator A5025363488 @default.
• W2141681955 creator A5026674508 @default.
• W2141681955 creator A5034189838 @default.
• W2141681955 creator A5057577421 @default.
• W2141681955 creator A5059847851 @default.
• W2141681955 creator A5079111307 @default.
• W2141681955 date "2013-03-01" @default.
• W2141681955 modified "2023-10-14" @default.
• W2141681955 title "Safety, efficacy and efficiency of laser-assisted IVF in subfertile mutant mouse strains" @default.
• W2141681955 cites W1526089040 @default.
• W2141681955 cites W1677982701 @default.
• W2141681955 cites W1967866238 @default.
• W2141681955 cites W1986570767 @default.
• W2141681955 cites W2017173928 @default.
• W2141681955 cites W2035971371 @default.
• W2141681955 cites W2036134437 @default.
• W2141681955 cites W2052063749 @default.
• W2141681955 cites W2055589222 @default.
• W2141681955 cites W2056444694 @default.
• W2141681955 cites W2064857735 @default.
• W2141681955 cites W2070319253 @default.
• W2141681955 cites W2086652031 @default.
• W2141681955 cites W2096173696 @default.
• W2141681955 cites W2100746446 @default.
• W2141681955 cites W2101149363 @default.
• W2141681955 cites W2108901477 @default.
• W2141681955 cites W2129830757 @default.
• W2141681955 cites W2139475636 @default.
• W2141681955 cites W2139627723 @default.
• W2141681955 cites W2151507041 @default.
• W2141681955 cites W2155582257 @default.
• W2141681955 cites W2162960932 @default.
• W2141681955 cites W2344467635 @default.
• W2141681955 cites W35687507 @default.
• W2141681955 cites W4234708366 @default.
• W2141681955 cites W59880327 @default.
• W2141681955 cites W89770524 @default.
• W2141681955 doi "https://doi.org/10.1530/rep-12-0477" @default.
• W2141681955 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3738198" @default.
• W2141681955 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23315689" @default.
• W2141681955 hasPublicationYear "2013" @default.
• W2141681955 type Work @default.
• W2141681955 sameAs 2141681955 @default.
• W2141681955 citedByCount "9" @default.
• W2141681955 countsByYear W21416819552014 @default.
• W2141681955 countsByYear W21416819552015 @default.
• W2141681955 countsByYear W21416819552016 @default.
• W2141681955 countsByYear W21416819552017 @default.
• W2141681955 countsByYear W21416819552018 @default.
• W2141681955 countsByYear W21416819552019 @default.
• W2141681955 countsByYear W21416819552020 @default.
• W2141681955 countsByYear W21416819552021 @default.
• W2141681955 crossrefType "journal-article" @default.
• W2141681955 hasAuthorship W2141681955A5013897171 @default.
• W2141681955 hasAuthorship W2141681955A5024804141 @default.
• W2141681955 hasAuthorship W2141681955A5025363488 @default.
• W2141681955 hasAuthorship W2141681955A5026674508 @default.
• W2141681955 hasAuthorship W2141681955A5034189838 @default.
• W2141681955 hasAuthorship W2141681955A5057577421 @default.
• W2141681955 hasAuthorship W2141681955A5059847851 @default.
• W2141681955 hasAuthorship W2141681955A5079111307 @default.
• W2141681955 hasBestOaLocation W21416819551 @default.
• W2141681955 hasConcept C105702510 @default.
• W2141681955 hasConcept C160099875 @default.
• W2141681955 hasConcept C16685009 @default.
• W2141681955 hasConcept C185592680 @default.
• W2141681955 hasConcept C196843134 @default.
• W2141681955 hasConcept C2776010925 @default.
• W2141681955 hasConcept C2776690073 @default.
• W2141681955 hasConcept C2777688143 @default.
• W2141681955 hasConcept C2779234561 @default.
• W2141681955 hasConcept C512716672 @default.
• W2141681955 hasConcept C54355233 @default.
• W2141681955 hasConcept C71924100 @default.
• W2141681955 hasConcept C86803240 @default.
• W2141681955 hasConcept C88972607 @default.
• W2141681955 hasConceptScore W2141681955C105702510 @default.
• W2141681955 hasConceptScore W2141681955C160099875 @default.
• W2141681955 hasConceptScore W2141681955C16685009 @default.
• W2141681955 hasConceptScore W2141681955C185592680 @default.
• W2141681955 hasConceptScore W2141681955C196843134 @default.
• W2141681955 hasConceptScore W2141681955C2776010925 @default.
• W2141681955 hasConceptScore W2141681955C2776690073 @default.
• W2141681955 hasConceptScore W2141681955C2777688143 @default.
• W2141681955 hasConceptScore W2141681955C2779234561 @default.
• W2141681955 hasConceptScore W2141681955C512716672 @default.
• W2141681955 hasConceptScore W2141681955C54355233 @default.
• W2141681955 hasConceptScore W2141681955C71924100 @default.
• W2141681955 hasConceptScore W2141681955C86803240 @default.
• W2141681955 hasConceptScore W2141681955C88972607 @default.
• W2141681955 hasIssue "3" @default.
• W2141681955 hasLocation W21416819551 @default.
• W2141681955 hasLocation W21416819552 @default.
• W2141681955 hasLocation W21416819553 @default.

• next