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- W2141717775 endingPage "643" @default.
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- W2141717775 abstract "ABSTRACT Pseudomonas aeruginosa ExoS is a type III-secreted type III-secreted, bifunctional protein that causes diverse effects on eukaryotic cell function. The coculture of P. aeruginosa strains expressing ExoS with HL-60 myeloid cells revealed the cell line to be resistant to the toxic effects of ExoS. Differentiation of HL-60 cells with phorbol 12-myristate 13-acetate (TPA) rendered the cell line sensitive to ExoS. To understand the cellular basis for the alteration in sensitivity, undifferentiated and TPA-differentiated HL-60 cells were compared for differences in bacterial adherence, type III secretion induction, and ExoS translocation. These comparisons found that ExoS was translocated more efficiently in TPA-differentiated HL-60 cells than in undifferentiated cells. The studies support the ability of eukaryotic cells to influence P. aeruginosa TTS at the level of membrane translocation." @default.
- W2141717775 created "2016-06-24" @default.
- W2141717775 creator A5014720458 @default.
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- W2141717775 date "2005-01-01" @default.
- W2141717775 modified "2023-09-23" @default.
- W2141717775 title "Characterization of an ExoS Type III Translocation-Resistant Cell Line" @default.
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- W2141717775 doi "https://doi.org/10.1128/iai.73.1.638-643.2005" @default.
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