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• W2141765718 abstract "Divergent selection has created rat phenotypes of high- and low-capacity runners (HCR and LCR, respectively) that have differences in aerobic capacity and correlated traits such as adiposity. We analyzed visceral adipose tissue of HCR and LCR using label-free high-definition MS (elevated energy) profiling. The running capacity of HCR was ninefold greater than LCR. Proteome profiling encompassed 448 proteins and detected 30 significant (p <0.05; false discovery rate <10%, calculated using q-values) differences. Approximately half of the proteins analyzed were of mitochondrial origin, but there were no significant differences in the abundance of proteins involved in aerobic metabolism. Instead, adipose tissue of LCR rats exhibited greater abundances of proteins associated with adipogenesis (e.g. cathepsin D), ER stress (e.g. 78 kDa glucose response protein), and inflammation (e.g. Ig gamma-2B chain C region). Whereas the abundance antioxidant enzymes such as superoxide dismutase [Cu-Zn] was greater in HCR tissue. Putative adipokines were also detected, in particular protein S100-B, was 431% more abundant in LCR adipose tissue. These findings reveal low running capacity is associated with a pathological profile in visceral adipose tissue proteome despite no detectable differences in mitochondrial protein abundance." @default.
• W2141765718 created "2016-06-24" @default.
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• W2141765718 date "2015-05-12" @default.
• W2141765718 modified "2023-10-09" @default.
• W2141765718 title "Label‐free profiling of white adipose tissue of rats exhibiting high or low levels of intrinsic exercise capacity" @default.
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• W2141765718 doi "https://doi.org/10.1002/pmic.201400537" @default.
• W2141765718 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4500166" @default.
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• W2141765718 hasPublicationYear "2015" @default.
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