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- W2141777993 abstract "A whole cell biocatalytic process was developed to enable the efficient oxidation of styrene to chiral (S)-styrene oxide with an enantiomeric excess better than 99%. Recombinant Escherichia coli cells were employed to express the genes styAB encoding the styrene monooxygenase of Pseudomonas sp. strain VLB120 from an expression plasmid utilizing the alk regulatory system of P. oleovorans GPo1. The strains reached specific activities of up to 70 U* (g cell dry weight)(-1) in shake-flask experiments with glucose as the carbon source. An efficient two-liquid phase fed-batch process was established for the production of (S)-styrene oxide with hexadecane as an apolar carrier solvent and a nutrient feed consisting of glucose, magnesium sulfate, and yeast extract. Engineering of the phase fraction and the composition of organic phase and feed led to a 2-L scale process with maximal volumetric productivities of 2.2 g (S)-styrene oxide per liter liquid volume per hour. This optimized process was based completely on defined medium and used bis(2-ethylhexyl)phthalate as the apolar carrier solvent, which together with substrate and inducer consisted of 50% of the total liquid volume. Using this system, we were able to produce per liter liquid volume 11 g of enantiopure (S)-styrene oxide in 10 h." @default.
- W2141777993 created "2016-06-24" @default.
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- W2141777993 date "2000-07-05" @default.
- W2141777993 modified "2023-10-16" @default.
- W2141777993 title "Production of enantiopure styrene oxide by recombinantEscherichia coli synthesizing a two-component styrene monooxygenase" @default.
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- W2141777993 doi "https://doi.org/10.1002/(sici)1097-0290(20000705)69:1<91::aid-bit11>3.0.co;2-x" @default.
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