FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2141978225> ?p ?o ?g. }

• W2141978225 endingPage "498" @default.
• W2141978225 startingPage "490" @default.
• W2141978225 abstract "The heme-containing catalase HPII of Escherichia coli consists of a homotetramer in which each subunit contains a core region with the highly conserved catalase tertiary structure, to which are appended N- and C-terminal extensions making it the largest known catalase. HPII does not bind NADPH, a cofactor often found in catalases. In HPII, residues 585–590 of the C-terminal extension protrude into the pocket corresponding to the NADPH binding site in the bovine liver catalase. Despite this difference, residues that define the NADPH pocket in the bovine enzyme appear to be well preserved in HPII. Only two residues that interact ionically with NADPH in the bovine enzyme (Asp212 and His304) differ in HPII (Glu270 and Glu362), but their mutation to the bovine sequence did not promote nucleotide binding. The active-site heme groups are deeply buried inside the molecular structure requiring the movement of substrate and products through long channels. One potential channel is about 30 Å in length, approaches the heme active site laterally, and is structurally related to the branched channel associated with the NADPH binding pocket in catalases that bind the dinucleotide. In HPII, the upper branch of this channel is interrupted by the presence of Arg260 ionically bound to Glu270. When Arg260 is replaced by alanine, there is a threefold increase in the catalytic activity of the enzyme. Inhibitors of HPII, including azide, cyanide, various sulfhydryl reagents, and alkylhydroxylamine derivatives, are effective at lower concentration on the Ala260 mutant enzyme compared to the wild-type enzyme. The crystal structure of the Ala260 mutant variant of HPII, determined at 2.3 Å resolution, revealed a number of local structural changes resulting in the opening of a second branch in the lateral channel, which appears to be used by inhibitors for access to the active site, either as an inlet channel for substrate or an exhaust channel for reaction products." @default.
• W2141978225 created "2016-06-24" @default.
• W2141978225 creator A5014882052 @default.
• W2141978225 creator A5034077469 @default.
• W2141978225 creator A5056355915 @default.
• W2141978225 creator A5058297240 @default.
• W2141978225 creator A5063198761 @default.
• W2141978225 date "2008-12-31" @default.
• W2141978225 modified "2023-10-03" @default.
• W2141978225 title "Role of the lateral channel in catalase HPII of Escherichia coli" @default.
• W2141978225 cites W1491314483 @default.
• W2141978225 cites W1500184452 @default.
• W2141978225 cites W1539796472 @default.
• W2141978225 cites W1796522495 @default.
• W2141978225 cites W1970723316 @default.
• W2141978225 cites W1987487572 @default.
• W2141978225 cites W1990194453 @default.
• W2141978225 cites W2004110672 @default.
• W2141978225 cites W2005624184 @default.
• W2141978225 cites W2012435471 @default.
• W2141978225 cites W2013083986 @default.
• W2141978225 cites W2015301923 @default.
• W2141978225 cites W2018499526 @default.
• W2141978225 cites W2021235777 @default.
• W2141978225 cites W2026565927 @default.
• W2141978225 cites W2027185514 @default.
• W2141978225 cites W2028622989 @default.
• W2141978225 cites W2034570625 @default.
• W2141978225 cites W2035293058 @default.
• W2141978225 cites W2042858344 @default.
• W2141978225 cites W2056613668 @default.
• W2141978225 cites W2092434707 @default.
• W2141978225 cites W2103794058 @default.
• W2141978225 cites W2116978023 @default.
• W2141978225 cites W2123028455 @default.
• W2141978225 cites W2133624234 @default.
• W2141978225 cites W2134462559 @default.
• W2141978225 cites W2135125477 @default.
• W2141978225 cites W2138270253 @default.
• W2141978225 cites W2154454183 @default.
• W2141978225 cites W2156166501 @default.
• W2141978225 cites W2160085559 @default.
• W2141978225 cites W2160125855 @default.
• W2141978225 cites W2235129190 @default.
• W2141978225 cites W2888511473 @default.
• W2141978225 doi "https://doi.org/10.1110/ps.8.3.490" @default.
• W2141978225 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2144281" @default.
• W2141978225 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10091651" @default.
• W2141978225 hasPublicationYear "2008" @default.
• W2141978225 type Work @default.
• W2141978225 sameAs 2141978225 @default.
• W2141978225 citedByCount "29" @default.
• W2141978225 countsByYear W21419782252012 @default.
• W2141978225 countsByYear W21419782252013 @default.
• W2141978225 countsByYear W21419782252014 @default.
• W2141978225 countsByYear W21419782252016 @default.
• W2141978225 countsByYear W21419782252017 @default.
• W2141978225 countsByYear W21419782252018 @default.
• W2141978225 countsByYear W21419782252019 @default.
• W2141978225 countsByYear W21419782252020 @default.
• W2141978225 countsByYear W21419782252021 @default.
• W2141978225 crossrefType "journal-article" @default.
• W2141978225 hasAuthorship W2141978225A5014882052 @default.
• W2141978225 hasAuthorship W2141978225A5034077469 @default.
• W2141978225 hasAuthorship W2141978225A5056355915 @default.
• W2141978225 hasAuthorship W2141978225A5058297240 @default.
• W2141978225 hasAuthorship W2141978225A5063198761 @default.
• W2141978225 hasBestOaLocation W21419782252 @default.
• W2141978225 hasConcept C104292427 @default.
• W2141978225 hasConcept C104317684 @default.
• W2141978225 hasConcept C107824862 @default.
• W2141978225 hasConcept C181199279 @default.
• W2141978225 hasConcept C185592680 @default.
• W2141978225 hasConcept C197957613 @default.
• W2141978225 hasConcept C2776217839 @default.
• W2141978225 hasConcept C2778481663 @default.
• W2141978225 hasConcept C2778979269 @default.
• W2141978225 hasConcept C2779268744 @default.
• W2141978225 hasConcept C41183919 @default.
• W2141978225 hasConcept C547475151 @default.
• W2141978225 hasConcept C55493867 @default.
• W2141978225 hasConcept C71240020 @default.
• W2141978225 hasConceptScore W2141978225C104292427 @default.
• W2141978225 hasConceptScore W2141978225C104317684 @default.
• W2141978225 hasConceptScore W2141978225C107824862 @default.
• W2141978225 hasConceptScore W2141978225C181199279 @default.
• W2141978225 hasConceptScore W2141978225C185592680 @default.
• W2141978225 hasConceptScore W2141978225C197957613 @default.
• W2141978225 hasConceptScore W2141978225C2776217839 @default.
• W2141978225 hasConceptScore W2141978225C2778481663 @default.
• W2141978225 hasConceptScore W2141978225C2778979269 @default.
• W2141978225 hasConceptScore W2141978225C2779268744 @default.
• W2141978225 hasConceptScore W2141978225C41183919 @default.
• W2141978225 hasConceptScore W2141978225C547475151 @default.
• W2141978225 hasConceptScore W2141978225C55493867 @default.
• W2141978225 hasConceptScore W2141978225C71240020 @default.
• W2141978225 hasIssue "3" @default.
• W2141978225 hasLocation W21419782251 @default.

• next