Matches in SemOpenAlex for { <https://semopenalex.org/work/W2142076550> ?p ?o ?g. }
Showing items 1 to 100 of
100
with 100 items per page.
- W2142076550 endingPage "436" @default.
- W2142076550 startingPage "429" @default.
- W2142076550 abstract "Abstract The purpose of this study is to determine the feasibility of the direct matrix‐assisted laser desorption/ionization (MALDI) identification of proteins in fixed T47D breast cancer cells and murine brain tissues. The ability to identify proteins from cells and tissue may lead to biomarkers that effectively predict the onset of defined disease states, and their dynamic behavior could be an important hint for drug target discoveries. Direct tissue application of trypsin allows protein identification in cells and tissues, while maintaining spatial integrity and intracellular organization. Using a chemical printer, matrix was co‐registered on trypsinized human T47D breast cancer cells and cryo‐preserved sections of murine brain tissue, followed by MALDI post‐source decay (PSD) or MALDI collision‐induced dissociation (CID), respectively. Mass‐to‐charge ( m/z ) data from the cells and brain tissues were processed using Mascot© software interrogation of the National Center for Biotechnology Information (NCBI) database. Histone H2B was identified from cultured T47D human breast cancer cells. Tubulin β 2 was identified from mouse brain cortex following an induced stroke. These results suggest that MALDI PSD/CID, combined with bioinformatics, can be used for the direct identification of proteins from cells and tissues. Refinements in preparation techniques may improve this approach to provide a tool for quantitative proteomics and clinical analysis. Copyright © 2007 John Wiley & Sons, Ltd." @default.
- W2142076550 created "2016-06-24" @default.
- W2142076550 creator A5007130830 @default.
- W2142076550 creator A5010519845 @default.
- W2142076550 creator A5015325500 @default.
- W2142076550 creator A5025758657 @default.
- W2142076550 creator A5026104436 @default.
- W2142076550 creator A5029637705 @default.
- W2142076550 creator A5030184373 @default.
- W2142076550 creator A5083498962 @default.
- W2142076550 date "2007-01-11" @default.
- W2142076550 modified "2023-09-30" @default.
- W2142076550 title "Direct identification of proteins from T47D cells and murine brain tissue by matrix-assisted laser desorption/ionization post-source decay/collision-induced dissociation" @default.
- W2142076550 cites W1590947463 @default.
- W2142076550 cites W1599833391 @default.
- W2142076550 cites W1963680299 @default.
- W2142076550 cites W1970209157 @default.
- W2142076550 cites W1972830102 @default.
- W2142076550 cites W1976720964 @default.
- W2142076550 cites W1982044520 @default.
- W2142076550 cites W1988731759 @default.
- W2142076550 cites W2004234097 @default.
- W2142076550 cites W2013521082 @default.
- W2142076550 cites W2020495753 @default.
- W2142076550 cites W2033278243 @default.
- W2142076550 cites W2042018422 @default.
- W2142076550 cites W2044214494 @default.
- W2142076550 cites W2053107065 @default.
- W2142076550 cites W2054493863 @default.
- W2142076550 cites W2054792262 @default.
- W2142076550 cites W2060310324 @default.
- W2142076550 cites W2069637270 @default.
- W2142076550 cites W2077476545 @default.
- W2142076550 cites W2082252497 @default.
- W2142076550 cites W2084378733 @default.
- W2142076550 cites W2086615965 @default.
- W2142076550 cites W2089706020 @default.
- W2142076550 cites W2134923404 @default.
- W2142076550 cites W2139564441 @default.
- W2142076550 cites W2141085947 @default.
- W2142076550 cites W2150155174 @default.
- W2142076550 cites W2155079126 @default.
- W2142076550 cites W2410607040 @default.
- W2142076550 doi "https://doi.org/10.1002/rcm.2849" @default.
- W2142076550 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17216666" @default.
- W2142076550 hasPublicationYear "2007" @default.
- W2142076550 type Work @default.
- W2142076550 sameAs 2142076550 @default.
- W2142076550 citedByCount "6" @default.
- W2142076550 crossrefType "journal-article" @default.
- W2142076550 hasAuthorship W2142076550A5007130830 @default.
- W2142076550 hasAuthorship W2142076550A5010519845 @default.
- W2142076550 hasAuthorship W2142076550A5015325500 @default.
- W2142076550 hasAuthorship W2142076550A5025758657 @default.
- W2142076550 hasAuthorship W2142076550A5026104436 @default.
- W2142076550 hasAuthorship W2142076550A5029637705 @default.
- W2142076550 hasAuthorship W2142076550A5030184373 @default.
- W2142076550 hasAuthorship W2142076550A5083498962 @default.
- W2142076550 hasConcept C104317684 @default.
- W2142076550 hasConcept C169760540 @default.
- W2142076550 hasConcept C181199279 @default.
- W2142076550 hasConcept C185592680 @default.
- W2142076550 hasConcept C2780340462 @default.
- W2142076550 hasConcept C2986936838 @default.
- W2142076550 hasConcept C46111723 @default.
- W2142076550 hasConcept C55493867 @default.
- W2142076550 hasConcept C70721500 @default.
- W2142076550 hasConcept C86803240 @default.
- W2142076550 hasConceptScore W2142076550C104317684 @default.
- W2142076550 hasConceptScore W2142076550C169760540 @default.
- W2142076550 hasConceptScore W2142076550C181199279 @default.
- W2142076550 hasConceptScore W2142076550C185592680 @default.
- W2142076550 hasConceptScore W2142076550C2780340462 @default.
- W2142076550 hasConceptScore W2142076550C2986936838 @default.
- W2142076550 hasConceptScore W2142076550C46111723 @default.
- W2142076550 hasConceptScore W2142076550C55493867 @default.
- W2142076550 hasConceptScore W2142076550C70721500 @default.
- W2142076550 hasConceptScore W2142076550C86803240 @default.
- W2142076550 hasIssue "3" @default.
- W2142076550 hasLocation W21420765501 @default.
- W2142076550 hasLocation W21420765502 @default.
- W2142076550 hasOpenAccess W2142076550 @default.
- W2142076550 hasPrimaryLocation W21420765501 @default.
- W2142076550 hasRelatedWork W2014093205 @default.
- W2142076550 hasRelatedWork W2065986307 @default.
- W2142076550 hasRelatedWork W2080856312 @default.
- W2142076550 hasRelatedWork W2088324202 @default.
- W2142076550 hasRelatedWork W2153167812 @default.
- W2142076550 hasRelatedWork W2884823586 @default.
- W2142076550 hasRelatedWork W2955258500 @default.
- W2142076550 hasRelatedWork W2996104802 @default.
- W2142076550 hasRelatedWork W4378532717 @default.
- W2142076550 hasRelatedWork W4386215772 @default.
- W2142076550 hasVolume "21" @default.
- W2142076550 isParatext "false" @default.
- W2142076550 isRetracted "false" @default.
- W2142076550 magId "2142076550" @default.
- W2142076550 workType "article" @default.