FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2142078911> ?p ?o ?g. }

• W2142078911 endingPage "955" @default.
• W2142078911 startingPage "946" @default.
• W2142078911 abstract "Abstract The manuscript presents definitive studies of surfactant protein D (SP-D) in the context of inflammatory lung fluids. The extent of SP-D depletion in bronchoalveolar lavage fluid (BALF) of children affected with cystic fibrosis (CF) is demonstrated to correlate best with the presence of the active neutrophil serine protease (NSP) elastase. Novel C-terminal SP-D fragments of 27 kDa and 11 kDa were identified in patient lavage fluid in addition to the previously described N-terminal, 35-kDa fragment by the use of isoelectrofocusing, modified blotting conditions, and region-specific antibodies. SP-D cleavage sites were identified. In vitro treatment of recombinant human SP-D dodecamers with NSPs replicated the fragmentation, but unexpectedly, the pattern of SP-D fragments generated by NSPs was dependent on calcium concentration. Whereas the 35- and 11-kDa fragments were generated when incubations were performed in low calcium (200 μM CaCl2), incubations in physiological calcium (2 mM) with higher amounts of elastase or proteinase-3 generated C-terminal 27, 21, and 14 kDa fragments, representing cleavage within the collagen and neck regions. Studies in which recombinant SP-D cleavage by individual NSPs was quantitatively evaluated under low and high calcium conditions showed that the most potent NSP for cleaving SP-D is elastase, followed by proteinase-3, followed by cathepsin G. These relative potency findings were considered in the context of other studies that showed that active NSPs in CF BALF are in the order: elastase, followed by cathepsin G, followed by proteinase-3. The findings support a pre-eminent role for neutrophil elastase as the critical protease responsible for SP-D depletion in inflammatory lung disease." @default.
• W2142078911 created "2016-06-24" @default.
• W2142078911 creator A5059314993 @default.
• W2142078911 creator A5080753315 @default.
• W2142078911 creator A5083340813 @default.
• W2142078911 creator A5088462075 @default.
• W2142078911 creator A5089991342 @default.
• W2142078911 date "2008-01-22" @default.
• W2142078911 modified "2023-10-12" @default.
• W2142078911 title "Patterns of neutrophil serine protease-dependent cleavage of surfactant protein D in inflammatory lung disease" @default.
• W2142078911 cites W1521863806 @default.
• W2142078911 cites W1588736007 @default.
• W2142078911 cites W1617119238 @default.
• W2142078911 cites W1929879919 @default.
• W2142078911 cites W1960770061 @default.
• W2142078911 cites W1969921713 @default.
• W2142078911 cites W1973762483 @default.
• W2142078911 cites W1980017808 @default.
• W2142078911 cites W1984783369 @default.
• W2142078911 cites W1990864962 @default.
• W2142078911 cites W1991202024 @default.
• W2142078911 cites W1997115286 @default.
• W2142078911 cites W2010629864 @default.
• W2142078911 cites W2013660192 @default.
• W2142078911 cites W2019354571 @default.
• W2142078911 cites W2022362501 @default.
• W2142078911 cites W2029268245 @default.
• W2142078911 cites W2040500344 @default.
• W2142078911 cites W2042439280 @default.
• W2142078911 cites W2055196479 @default.
• W2142078911 cites W2055857822 @default.
• W2142078911 cites W2059558881 @default.
• W2142078911 cites W2064758742 @default.
• W2142078911 cites W2067127493 @default.
• W2142078911 cites W2069547352 @default.
• W2142078911 cites W2070364452 @default.
• W2142078911 cites W2077229514 @default.
• W2142078911 cites W2078380427 @default.
• W2142078911 cites W2080077176 @default.
• W2142078911 cites W2089470164 @default.
• W2142078911 cites W2101075069 @default.
• W2142078911 cites W2102249376 @default.
• W2142078911 cites W2108935488 @default.
• W2142078911 cites W2123268390 @default.
• W2142078911 cites W2124366530 @default.
• W2142078911 cites W2128714994 @default.
• W2142078911 cites W2144918455 @default.
• W2142078911 cites W2145082642 @default.
• W2142078911 cites W2163611747 @default.
• W2142078911 cites W2168881969 @default.
• W2142078911 cites W4235251147 @default.
• W2142078911 cites W4245546006 @default.
• W2142078911 doi "https://doi.org/10.1189/jlb.1007684" @default.
• W2142078911 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18211966" @default.
• W2142078911 hasPublicationYear "2008" @default.
• W2142078911 type Work @default.
• W2142078911 sameAs 2142078911 @default.
• W2142078911 citedByCount "38" @default.
• W2142078911 countsByYear W21420789112012 @default.
• W2142078911 countsByYear W21420789112013 @default.
• W2142078911 countsByYear W21420789112014 @default.
• W2142078911 countsByYear W21420789112015 @default.
• W2142078911 countsByYear W21420789112016 @default.
• W2142078911 countsByYear W21420789112017 @default.
• W2142078911 countsByYear W21420789112018 @default.
• W2142078911 countsByYear W21420789112019 @default.
• W2142078911 countsByYear W21420789112021 @default.
• W2142078911 countsByYear W21420789112023 @default.
• W2142078911 crossrefType "journal-article" @default.
• W2142078911 hasAuthorship W2142078911A5059314993 @default.
• W2142078911 hasAuthorship W2142078911A5080753315 @default.
• W2142078911 hasAuthorship W2142078911A5083340813 @default.
• W2142078911 hasAuthorship W2142078911A5088462075 @default.
• W2142078911 hasAuthorship W2142078911A5089991342 @default.
• W2142078911 hasBestOaLocation W21420789111 @default.
• W2142078911 hasConcept C104317684 @default.
• W2142078911 hasConcept C138885662 @default.
• W2142078911 hasConcept C151730666 @default.
• W2142078911 hasConcept C153911025 @default.
• W2142078911 hasConcept C159654299 @default.
• W2142078911 hasConcept C163764329 @default.
• W2142078911 hasConcept C173633252 @default.
• W2142078911 hasConcept C175156509 @default.
• W2142078911 hasConcept C181199279 @default.
• W2142078911 hasConcept C203014093 @default.
• W2142078911 hasConcept C21194631 @default.
• W2142078911 hasConcept C2776317360 @default.
• W2142078911 hasConcept C2776326425 @default.
• W2142078911 hasConcept C2776714187 @default.
• W2142078911 hasConcept C2776914184 @default.
• W2142078911 hasConcept C2777132095 @default.
• W2142078911 hasConcept C2777714996 @default.
• W2142078911 hasConcept C2777807008 @default.
• W2142078911 hasConcept C2777961210 @default.
• W2142078911 hasConcept C2781044401 @default.
• W2142078911 hasConcept C40767141 @default.
• W2142078911 hasConcept C41895202 @default.
• W2142078911 hasConcept C43369102 @default.

• next