Matches in SemOpenAlex for { <https://semopenalex.org/work/W2142285381> ?p ?o ?g. }
Showing items 1 to 93 of
93
with 100 items per page.
- W2142285381 endingPage "595" @default.
- W2142285381 startingPage "588" @default.
- W2142285381 abstract "The use of dideoxynucleotide triphosphates labeled with different fluorescent dyes (dye terminators) is the most versatile method for automated DNA sequencing. However, variation in peak heights reduces base-calling accuracy and limits heterozygous allele detection, favoring use of dye-labeled primers for this purpose. We have discovered that the addition of a manganese salt to the PE Applied Biosystems dye-terminator sequencing kits overcomes these limitations for the older rhodamine dyes as well as the more recent dichloro-rhodamine dyes (dRhodamine and BigDyes). Addition of manganese to reactions containing dRhodamine-based dye terminators produced the highest base-calling accuracy. This combination resulted in the most uniform electropherogram profiles, superior to those produced by BigDye terminators and published for dye primers, and facilitated detection of heterozygous alleles." @default.
- W2142285381 created "2016-06-24" @default.
- W2142285381 creator A5036812326 @default.
- W2142285381 creator A5087213703 @default.
- W2142285381 date "1999-06-01" @default.
- W2142285381 modified "2023-10-18" @default.
- W2142285381 title "Improved DNA Sequencing Accuracy and Detection of Heterozygous Alleles Using Manganese Citrate and Different Fluorescent Dye Terminators" @default.
- W2142285381 cites W120392574 @default.
- W2142285381 cites W14357638 @default.
- W2142285381 cites W1487576512 @default.
- W2142285381 cites W1977636724 @default.
- W2142285381 cites W1988676851 @default.
- W2142285381 cites W2025735321 @default.
- W2142285381 cites W2026491313 @default.
- W2142285381 cites W2028989854 @default.
- W2142285381 cites W2031928161 @default.
- W2142285381 cites W2061979195 @default.
- W2142285381 cites W2066076729 @default.
- W2142285381 cites W2073484256 @default.
- W2142285381 cites W2076536222 @default.
- W2142285381 cites W2106350699 @default.
- W2142285381 cites W2116761032 @default.
- W2142285381 cites W2400299980 @default.
- W2142285381 cites W80436974 @default.
- W2142285381 cites W1993618544 @default.
- W2142285381 doi "https://doi.org/10.1101/gr.9.6.588" @default.
- W2142285381 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/310770" @default.
- W2142285381 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10400927" @default.
- W2142285381 hasPublicationYear "1999" @default.
- W2142285381 type Work @default.
- W2142285381 sameAs 2142285381 @default.
- W2142285381 citedByCount "15" @default.
- W2142285381 countsByYear W21422853812013 @default.
- W2142285381 countsByYear W21422853812015 @default.
- W2142285381 countsByYear W21422853812021 @default.
- W2142285381 crossrefType "journal-article" @default.
- W2142285381 hasAuthorship W2142285381A5036812326 @default.
- W2142285381 hasAuthorship W2142285381A5087213703 @default.
- W2142285381 hasBestOaLocation W21422853811 @default.
- W2142285381 hasConcept C104317684 @default.
- W2142285381 hasConcept C116403925 @default.
- W2142285381 hasConcept C121332964 @default.
- W2142285381 hasConcept C1276947 @default.
- W2142285381 hasConcept C153911025 @default.
- W2142285381 hasConcept C178046730 @default.
- W2142285381 hasConcept C180754005 @default.
- W2142285381 hasConcept C2779916640 @default.
- W2142285381 hasConcept C2900643 @default.
- W2142285381 hasConcept C44995494 @default.
- W2142285381 hasConcept C51679486 @default.
- W2142285381 hasConcept C54355233 @default.
- W2142285381 hasConcept C552990157 @default.
- W2142285381 hasConcept C62520636 @default.
- W2142285381 hasConcept C86803240 @default.
- W2142285381 hasConcept C91881484 @default.
- W2142285381 hasConceptScore W2142285381C104317684 @default.
- W2142285381 hasConceptScore W2142285381C116403925 @default.
- W2142285381 hasConceptScore W2142285381C121332964 @default.
- W2142285381 hasConceptScore W2142285381C1276947 @default.
- W2142285381 hasConceptScore W2142285381C153911025 @default.
- W2142285381 hasConceptScore W2142285381C178046730 @default.
- W2142285381 hasConceptScore W2142285381C180754005 @default.
- W2142285381 hasConceptScore W2142285381C2779916640 @default.
- W2142285381 hasConceptScore W2142285381C2900643 @default.
- W2142285381 hasConceptScore W2142285381C44995494 @default.
- W2142285381 hasConceptScore W2142285381C51679486 @default.
- W2142285381 hasConceptScore W2142285381C54355233 @default.
- W2142285381 hasConceptScore W2142285381C552990157 @default.
- W2142285381 hasConceptScore W2142285381C62520636 @default.
- W2142285381 hasConceptScore W2142285381C86803240 @default.
- W2142285381 hasConceptScore W2142285381C91881484 @default.
- W2142285381 hasIssue "6" @default.
- W2142285381 hasLocation W21422853811 @default.
- W2142285381 hasLocation W21422853812 @default.
- W2142285381 hasOpenAccess W2142285381 @default.
- W2142285381 hasPrimaryLocation W21422853811 @default.
- W2142285381 hasRelatedWork W1991523530 @default.
- W2142285381 hasRelatedWork W2002128513 @default.
- W2142285381 hasRelatedWork W2010403799 @default.
- W2142285381 hasRelatedWork W2020824267 @default.
- W2142285381 hasRelatedWork W2056764165 @default.
- W2142285381 hasRelatedWork W2057739827 @default.
- W2142285381 hasRelatedWork W2075354549 @default.
- W2142285381 hasRelatedWork W2078000306 @default.
- W2142285381 hasRelatedWork W2883371012 @default.
- W2142285381 hasRelatedWork W2092874662 @default.
- W2142285381 hasVolume "9" @default.
- W2142285381 isParatext "false" @default.
- W2142285381 isRetracted "false" @default.
- W2142285381 magId "2142285381" @default.
- W2142285381 workType "article" @default.