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- W2142451170 abstract "When uncoated polystyrene beads suspended in Mg-ATP solution were introduced into the internodal cell of an alga Chara corallina, the beads moved along the actin cables with directions and velocities (30-62 microns s-1) similar to those of native cytoplasmic streaming. Bead movement was inhibited both in the absence of ATP and in the presence of CA2+, as with native cytoplasmic streaming. These results indicate that bead movement is caused by cytoplasmic myosin molecules attached to the head surface interacting with actin cables. The steady-state force-velocity relationship of the actin-myosin sliding that produces cytoplasmic streaming was determined by applying constant centrifugal forces to the beads moving on the actin cables. The force-velocity curve in the positive load region was nearly straight, and the implications of this shape are discussed in connection with the kinetic properties of the actin-myosin interaction in cytoplasmic streaming. It is suggested that the time for which a cytoplasmic myosin head is detached from actin in one cycle of actin-myosin interaction is very short. The Ca(2+)-induced actin-myosin linkages, responsible for the Ca(2+)-induced stoppage of cytoplasmic streaming, were shown to be much stronger than the rigor actin-myosin linkages." @default.
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- W2142451170 date "1995-04-01" @default.
- W2142451170 modified "2023-10-17" @default.
- W2142451170 title "The Force–velocity Relationship of the Atp-Dependent Actin–Myosin Sliding Causing Cytoplasmic Streaming in Algal Cells, Studied Using a Centrifuge Microscope" @default.
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- W2142451170 doi "https://doi.org/10.1242/jeb.198.4.1021" @default.
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