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• W2142497923 endingPage "e42784" @default.
• W2142497923 startingPage "e42784" @default.
• W2142497923 abstract "The enzyme ribonucleotide reductase (RNR) catalyzes the conversion of ribonucleotides to deoxyribonucleotides, the precursors for DNA. RNR requires a thiyl radical to activate the substrate. In RNR of eukaryotes (class Ia RNR), this radical originates from a tyrosyl radical formed in reaction with oxygen (O2) and a ferrous di-iron center in RNR. The crucian carp (Carassius carassius) is one of very few vertebrates that can tolerate several months completely without oxygen (anoxia), a trait that enables this fish to survive under the ice in small ponds that become anoxic during the winter. Previous studies have found indications of cell division in this fish after 7 days of anoxia. This appears nearly impossible, as DNA synthesis requires the production of new deoxyribonucleotides and therefore active RNR. We have here characterized RNR in crucian carp, to search for adaptations to anoxia. We report the full-length sequences of two paralogs of each of the RNR subunits (R1i, R1ii, R2i, R2ii, p53R2i and p53R2ii), obtained by cloning and sequencing. The mRNA levels of these subunits were measured with quantitative PCR and were generally well maintained in hypoxia and anoxia in heart and brain. We also report maintained or increased mRNA levels of the cell division markers proliferating cell nuclear antigen (PCNA), brain derived neurotrophic factor (BDNF) and Ki67 in anoxic hearts and brains. Electron paramagnetic resonance (EPR) measurements on in vitro expressed crucian carp R2 and p53R2 proteins gave spectra similar to mammalian RNRs, including previously unpublished human and mouse p53R2 EPR spectra. However, the radicals in crucian carp RNR small subunits, especially in the p53R2ii subunit, were very stable at 0°C. A long half-life of the tyrosyl radical during wintertime anoxia could allow for continued cell division in crucian carp." @default.
• W2142497923 created "2016-06-24" @default.
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• W2142497923 date "2012-08-14" @default.
• W2142497923 modified "2023-10-16" @default.
• W2142497923 title "Studies of Ribonucleotide Reductase in Crucian Carp—An Oxygen Dependent Enzyme in an Anoxia Tolerant Vertebrate" @default.
• W2142497923 cites W1487470948 @default.
• W2142497923 cites W1499500424 @default.
• W2142497923 cites W1510505825 @default.
• W2142497923 cites W1536062643 @default.
• W2142497923 cites W1545921426 @default.
• W2142497923 cites W1580951859 @default.
• W2142497923 cites W1680634601 @default.
• W2142497923 cites W1937717811 @default.
• W2142497923 cites W1940574782 @default.
• W2142497923 cites W1963883745 @default.
• W2142497923 cites W1970676190 @default.
• W2142497923 cites W1971429162 @default.
• W2142497923 cites W1972415605 @default.
• W2142497923 cites W1973844844 @default.
• W2142497923 cites W1976187390 @default.
• W2142497923 cites W1977788936 @default.
• W2142497923 cites W1980723552 @default.
• W2142497923 cites W1982410689 @default.
• W2142497923 cites W1984353977 @default.
• W2142497923 cites W1993073412 @default.
• W2142497923 cites W1997810457 @default.
• W2142497923 cites W1998457769 @default.
• W2142497923 cites W2000150758 @default.
• W2142497923 cites W2002551641 @default.
• W2142497923 cites W2004850139 @default.
• W2142497923 cites W2008924348 @default.
• W2142497923 cites W2009486080 @default.
• W2142497923 cites W2012293009 @default.
• W2142497923 cites W2012914676 @default.
• W2142497923 cites W2015493876 @default.
• W2142497923 cites W2018066504 @default.
• W2142497923 cites W2018528100 @default.
• W2142497923 cites W2021313959 @default.
• W2142497923 cites W2025784676 @default.
• W2142497923 cites W2027477859 @default.
• W2142497923 cites W2031433002 @default.
• W2142497923 cites W2036873456 @default.
• W2142497923 cites W2041098156 @default.
• W2142497923 cites W2045039660 @default.
• W2142497923 cites W2047167565 @default.
• W2142497923 cites W2050094587 @default.
• W2142497923 cites W2050192518 @default.
• W2142497923 cites W2051630798 @default.
• W2142497923 cites W2054662394 @default.
• W2142497923 cites W2059866243 @default.
• W2142497923 cites W2060455152 @default.
• W2142497923 cites W2065052214 @default.
• W2142497923 cites W2066552121 @default.
• W2142497923 cites W2068456988 @default.
• W2142497923 cites W2069536606 @default.
• W2142497923 cites W2070326775 @default.
• W2142497923 cites W2072930187 @default.
• W2142497923 cites W2075423291 @default.
• W2142497923 cites W2080114721 @default.
• W2142497923 cites W2081359936 @default.
• W2142497923 cites W2082067144 @default.
• W2142497923 cites W2082820112 @default.
• W2142497923 cites W2085972813 @default.
• W2142497923 cites W2092167912 @default.
• W2142497923 cites W2111111533 @default.
• W2142497923 cites W2111909280 @default.
• W2142497923 cites W2113916199 @default.
• W2142497923 cites W2118941388 @default.
• W2142497923 cites W2120960764 @default.
• W2142497923 cites W2120992133 @default.
• W2142497923 cites W2126033753 @default.
• W2142497923 cites W2126342893 @default.
• W2142497923 cites W2131884667 @default.
• W2142497923 cites W2135123683 @default.
• W2142497923 cites W2136309995 @default.
• W2142497923 cites W2137845412 @default.
• W2142497923 cites W2148595039 @default.
• W2142497923 cites W2149548143 @default.
• W2142497923 cites W2151730731 @default.
• W2142497923 cites W2152301430 @default.
• W2142497923 cites W2152557783 @default.
• W2142497923 cites W2154035365 @default.
• W2142497923 cites W2154431541 @default.
• W2142497923 cites W2162210817 @default.
• W2142497923 cites W2280950052 @default.
• W2142497923 cites W2281835866 @default.
• W2142497923 cites W2398535294 @default.
• W2142497923 cites W2408296952 @default.

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