FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2142505369> ?p ?o ?g. }

• W2142505369 endingPage "4764" @default.
• W2142505369 startingPage "4754" @default.
• W2142505369 abstract "The mRNA cap-binding protein (eukaryotic initiation factor 4E [eIF4E]) binds the m7 GpppN cap on mRNA, thereby initiating translation. eIF4E is essential and rate limiting for protein synthesis. Overexpression of eIF4E transforms cells, and mutations in eIF4E arrest cells in G, in cdc33 mutants. In this work, we identified the promoter region of the gene encoding eIF4E, because we previously identified eIF4E as a potential myc-regulated gene. In support of our previous data, a minimal, functional, 403-nucleotide promoter region of eIF4E was found to contain CACGTG E box repeats, and this core eIF4E promoter was myc responsive in cotransfections with c-myc. A direct role for myc in activating the eIF4E promoter was demonstrated by cotransfections with two dominant negative mutants of c-myc (MycdeltaTAD and MycdeltaBR) which equally suppressed promoter function. Furthermore, electrophoretic mobility shift assays demonstrated quantitative binding to the E box motifs that correlated with myc levels in the electrophoretic mobility shift assay extracts; supershift assays demonstrated max and USF binding to the same motif. cis mutations in the core or flank of the eIF4E E box simultaneously altered myc-max and USF binding and inactivated the promoter. Indeed, mutations of this E box inactivated the promoter in all cells tested, suggesting it is essential for expression of eIF4E. Furthermore, the GGCCACGTG(A/T)C(C/G) sequence is shared with other in vivo targets for c-myc, but unlike other targets, it is located in the immediate promoter region. Its critical function in the eIF4E promoter coupled with the known functional significance of eIF4E in growth regulation makes it a particularly interesting target for c-myc regulation." @default.
• W2142505369 created "2016-06-24" @default.
• W2142505369 creator A5005019978 @default.
• W2142505369 creator A5009243530 @default.
• W2142505369 creator A5042503296 @default.
• W2142505369 creator A5043269990 @default.
• W2142505369 creator A5046623395 @default.
• W2142505369 creator A5060275984 @default.
• W2142505369 creator A5079570342 @default.
• W2142505369 creator A5080836420 @default.
• W2142505369 date "1996-09-01" @default.
• W2142505369 modified "2023-10-11" @default.
• W2142505369 title "An Essential E Box in the Promoter of the Gene Encoding the mRNA Cap-Binding Protein (Eukaryotic Initiation Factor 4E) Is a Target for Activation by c-myc" @default.
• W2142505369 cites W1230664046 @default.
• W2142505369 cites W128302075 @default.
• W2142505369 cites W137827881 @default.
• W2142505369 cites W1496420443 @default.
• W2142505369 cites W1499458587 @default.
• W2142505369 cites W1515792029 @default.
• W2142505369 cites W1516043257 @default.
• W2142505369 cites W1544068656 @default.
• W2142505369 cites W1549051606 @default.
• W2142505369 cites W1606834485 @default.
• W2142505369 cites W161464813 @default.
• W2142505369 cites W182248608 @default.
• W2142505369 cites W1828176999 @default.
• W2142505369 cites W1968075576 @default.
• W2142505369 cites W1968710429 @default.
• W2142505369 cites W1970497539 @default.
• W2142505369 cites W1974391793 @default.
• W2142505369 cites W1975489321 @default.
• W2142505369 cites W1980022529 @default.
• W2142505369 cites W1980290943 @default.
• W2142505369 cites W1982172428 @default.
• W2142505369 cites W1982598173 @default.
• W2142505369 cites W1989009382 @default.
• W2142505369 cites W1990367849 @default.
• W2142505369 cites W1991803500 @default.
• W2142505369 cites W1993243592 @default.
• W2142505369 cites W1997345702 @default.
• W2142505369 cites W1997614722 @default.
• W2142505369 cites W1997806157 @default.
• W2142505369 cites W2004205790 @default.
• W2142505369 cites W2008972719 @default.
• W2142505369 cites W2012398556 @default.
• W2142505369 cites W2013454002 @default.
• W2142505369 cites W2022081023 @default.
• W2142505369 cites W2031900820 @default.
• W2142505369 cites W2040193953 @default.
• W2142505369 cites W2042777921 @default.
• W2142505369 cites W2045821588 @default.
• W2142505369 cites W2047404819 @default.
• W2142505369 cites W2048555922 @default.
• W2142505369 cites W2053879589 @default.
• W2142505369 cites W2054876743 @default.
• W2142505369 cites W2056348907 @default.
• W2142505369 cites W2056627100 @default.
• W2142505369 cites W2059101624 @default.
• W2142505369 cites W2059433877 @default.
• W2142505369 cites W2060333964 @default.
• W2142505369 cites W2071887421 @default.
• W2142505369 cites W2082670705 @default.
• W2142505369 cites W2085213714 @default.
• W2142505369 cites W2088577765 @default.
• W2142505369 cites W2088665501 @default.
• W2142505369 cites W2096462919 @default.
• W2142505369 cites W2150868953 @default.
• W2142505369 cites W2155625886 @default.
• W2142505369 cites W2164107824 @default.
• W2142505369 cites W2164515753 @default.
• W2142505369 cites W2165055107 @default.
• W2142505369 cites W4236512674 @default.
• W2142505369 cites W67546116 @default.
• W2142505369 doi "https://doi.org/10.1128/mcb.16.9.4754" @default.
• W2142505369 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/231476" @default.
• W2142505369 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8756633" @default.
• W2142505369 hasPublicationYear "1996" @default.
• W2142505369 type Work @default.
• W2142505369 sameAs 2142505369 @default.
• W2142505369 citedByCount "223" @default.
• W2142505369 countsByYear W21425053692012 @default.
• W2142505369 countsByYear W21425053692013 @default.
• W2142505369 countsByYear W21425053692014 @default.
• W2142505369 countsByYear W21425053692015 @default.
• W2142505369 countsByYear W21425053692016 @default.
• W2142505369 countsByYear W21425053692017 @default.
• W2142505369 countsByYear W21425053692018 @default.
• W2142505369 countsByYear W21425053692019 @default.
• W2142505369 countsByYear W21425053692020 @default.
• W2142505369 countsByYear W21425053692021 @default.
• W2142505369 countsByYear W21425053692022 @default.
• W2142505369 countsByYear W21425053692023 @default.
• W2142505369 crossrefType "journal-article" @default.
• W2142505369 hasAuthorship W2142505369A5005019978 @default.
• W2142505369 hasAuthorship W2142505369A5009243530 @default.
• W2142505369 hasAuthorship W2142505369A5042503296 @default.
• W2142505369 hasAuthorship W2142505369A5043269990 @default.
• W2142505369 hasAuthorship W2142505369A5046623395 @default.

• next