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- W2142867177 abstract "DNA topoisomerases solve topological problems during chromosome metabolism. We investigated where and when Top1 and Top2 are recruited on replicating chromosomes and how their inactivation affects fork integrity and DNA damage checkpoint activation. We show that, in the context of replicating chromatin, Top1 and Top2 act within a 600-base-pair (bp) region spanning the moving forks. Top2 exhibits additional S-phase clusters at specific intergenic loci, mostly containing promoters. TOP1 ablation does not affect fork progression and stability and does not cause activation of the Rad53 checkpoint kinase. top2 mutants accumulate sister chromatid junctions in S phase without affecting fork progression and activate Rad53 at the M–G1 transition. top1 top2 double mutants exhibit fork block and processing and phosphorylation of Rad53 and γH2A in S phase. The exonuclease Exo1 influences fork processing and DNA damage checkpoint activation in top1 top2 mutants. Our data are consistent with a coordinated action of Top1 and Top2 in counteracting the accumulation of torsional stress and sister chromatid entanglement at replication forks, thus preventing the diffusion of topological changes along large chromosomal regions. A failure in resolving fork-related topological constrains during S phase may therefore result in abnormal chromosome transitions, DNA damage checkpoint activation, and chromosome breakage during segregation." @default.
- W2142867177 created "2016-06-24" @default.
- W2142867177 creator A5030754377 @default.
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- W2142867177 date "2007-08-01" @default.
- W2142867177 modified "2023-09-23" @default.
- W2142867177 title "Top1- and Top2-mediated topological transitions at replication forks ensure fork progression and stability and prevent DNA damage checkpoint activation" @default.
- W2142867177 cites W1493549734 @default.
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- W2142867177 cites W1564916342 @default.
- W2142867177 cites W1604458367 @default.
- W2142867177 cites W1660332899 @default.
- W2142867177 cites W1961693204 @default.
- W2142867177 cites W1969081551 @default.
- W2142867177 cites W1972138555 @default.
- W2142867177 cites W1973963465 @default.
- W2142867177 cites W1975001355 @default.
- W2142867177 cites W1975472631 @default.
- W2142867177 cites W1977088495 @default.
- W2142867177 cites W1978680674 @default.
- W2142867177 cites W1978696228 @default.
- W2142867177 cites W1989183743 @default.
- W2142867177 cites W1990035744 @default.
- W2142867177 cites W1991539934 @default.
- W2142867177 cites W1993623149 @default.
- W2142867177 cites W1994183178 @default.
- W2142867177 cites W1994810131 @default.
- W2142867177 cites W1999208409 @default.
- W2142867177 cites W2001372713 @default.
- W2142867177 cites W2004008931 @default.
- W2142867177 cites W2007025396 @default.
- W2142867177 cites W2008486131 @default.
- W2142867177 cites W2008992619 @default.
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- W2142867177 cites W2015000379 @default.
- W2142867177 cites W2018399328 @default.
- W2142867177 cites W2030357987 @default.
- W2142867177 cites W2032850988 @default.
- W2142867177 cites W2034460306 @default.
- W2142867177 cites W2035910115 @default.
- W2142867177 cites W2043028465 @default.
- W2142867177 cites W2043895957 @default.
- W2142867177 cites W2053374394 @default.
- W2142867177 cites W2055604308 @default.
- W2142867177 cites W2055667487 @default.
- W2142867177 cites W2055948417 @default.
- W2142867177 cites W2058911936 @default.
- W2142867177 cites W2066975479 @default.
- W2142867177 cites W2069396761 @default.
- W2142867177 cites W2073658080 @default.
- W2142867177 cites W2078288014 @default.
- W2142867177 cites W2086764112 @default.
- W2142867177 cites W2090311962 @default.
- W2142867177 cites W2091098747 @default.
- W2142867177 cites W2092638438 @default.
- W2142867177 cites W2097835632 @default.
- W2142867177 cites W2105222529 @default.
- W2142867177 cites W2109339776 @default.
- W2142867177 cites W2110555186 @default.
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- W2142867177 cites W2114351091 @default.
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- W2142867177 cites W2124624490 @default.
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- W2142867177 cites W2144764837 @default.
- W2142867177 cites W2156645202 @default.
- W2142867177 cites W2157508254 @default.
- W2142867177 cites W2164938051 @default.
- W2142867177 cites W2168839178 @default.
- W2142867177 cites W2170450010 @default.
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- W2142867177 doi "https://doi.org/10.1101/gad.432107" @default.
- W2142867177 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1935030" @default.
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