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- W2142903721 abstract "Maize (Zea mays L., cv. Samodek) callus cultures were exposed for long period (22 months) to lead (0.5 mM lead chloride) and lead content, oxidative damage and antioxidative response were evaluated at different steps. Inductively coupled plasma (ICP) emission spectroscopy analysis showed that lead entered the cells and it accumulated, but its internal concentration was maintained 10-fold less than the external one. Increase of both polyamine and lipid peroxide content indicated that cells underwent a stress condition due to an oxidative attack, counteracted by an increase of antioxidative defence enzyme activities, ascorbate peroxidase (APX, EC 1.11.1.11) and glutathione reductase (GR, EC 1.6.4.2). After 10 months, from the start of the lead treatment, a stock of calli was transferred for 6 months in a lead-deprived medium and then re-exposed to lead for a further 6 months. Analysis indicated that lead-deprived calli maintained high levels of APX and GR activities, suggesting that, over the experimental time–course, cells with high APX and GR activity were selected and allowed to enrich the cultures. These cultures, after a new lead treatment, showed a lower oxidative damage compared to continuously lead-treated calli." @default.
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- W2142903721 date "2003-01-01" @default.
- W2142903721 modified "2023-10-18" @default.
- W2142903721 title "Increased antioxidative capacity in maize calli during and after oxidative stress induced by a long lead treatment" @default.
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- W2142903721 doi "https://doi.org/10.1016/s0981-9428(02)00008-6" @default.
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