Matches in SemOpenAlex for { <https://semopenalex.org/work/W2142958665> ?p ?o ?g. }
Showing items 1 to 100 of
100
with 100 items per page.
- W2142958665 endingPage "28" @default.
- W2142958665 startingPage "10" @default.
- W2142958665 abstract "The ubiquitous redox cofactors nicotinamide adenine dinucleotides [NAD and NADP] are very similar molecules, despite their participation in substantially different biochemical processes. NADP differs from NAD in only the presence of an additional phosphate group esterified to the 2′-hydroxyl group of the ribose at the adenine end and yet NADP is confined with few exceptions to the reactions of reductive biosynthesis, whereas NAD is used almost exclusively in oxidative degradations. The discrimination between NAD and NADP is therefore an impressive example of the power of molecular recognition by proteins. The many known tertiary structures of NADP complexes affords the possibility for an analysis of their discrimination. A systematic analysis of several crystal structures of NAD(P)-protein complexes show that: 1) the NADP coenzymes are more flexible in conformation than those of NAD; 2) although the protein-cofactor interactions are largely conserved in the NAD complexes, they are quite variable in those of NADP; and 3) in both cases the pocket around the nicotinamide moiety is substrate dependent. The conserved and variable interactions between protein and cofactors in the respective binding pockets are reported in detail. Discrimination between NAD and NADP is essentially a consequence of the overall pocket and not of a few residues. A clear fingerprint in NAD complexes is a carboxylate side chain that chelates the diol group at the ribose near the adenine, whereas in NADP complexes an arginine side chain faces the adenine plane and interacts with the phosphomonoester. The latter type of interaction might be a general feature of recognition of nucleotides by proteins. Other features such as strand-like hydrogen bonding between the NADP diphosphate moeties and the protein are also significant. The NADP binding pocket properties should prove useful in protein engineering and design. © 1997 Wiley-Liss Inc." @default.
- W2142958665 created "2016-06-24" @default.
- W2142958665 creator A5063489663 @default.
- W2142958665 creator A5069990985 @default.
- W2142958665 date "1997-05-01" @default.
- W2142958665 modified "2023-10-18" @default.
- W2142958665 title "NADP-Dependent enzymes. I: Conserved stereochemistry of cofactor binding" @default.
- W2142958665 cites W1500138475 @default.
- W2142958665 cites W1966041739 @default.
- W2142958665 cites W1974176847 @default.
- W2142958665 cites W1986191025 @default.
- W2142958665 cites W1994409414 @default.
- W2142958665 cites W2000388754 @default.
- W2142958665 cites W2008317180 @default.
- W2142958665 cites W2012068802 @default.
- W2142958665 cites W2024979313 @default.
- W2142958665 cites W2028961289 @default.
- W2142958665 cites W2035294179 @default.
- W2142958665 cites W2035990611 @default.
- W2142958665 cites W2038533609 @default.
- W2142958665 cites W2058197852 @default.
- W2142958665 cites W2059890422 @default.
- W2142958665 cites W2060728728 @default.
- W2142958665 cites W2062129975 @default.
- W2142958665 cites W2062714518 @default.
- W2142958665 cites W2067022404 @default.
- W2142958665 cites W2077851224 @default.
- W2142958665 cites W2078763795 @default.
- W2142958665 cites W2080972330 @default.
- W2142958665 cites W2083988452 @default.
- W2142958665 cites W2095181192 @default.
- W2142958665 cites W2100498403 @default.
- W2142958665 cites W2108107014 @default.
- W2142958665 cites W2123949437 @default.
- W2142958665 cites W2157165549 @default.
- W2142958665 cites W2328791255 @default.
- W2142958665 cites W2949092920 @default.
- W2142958665 doi "https://doi.org/10.1002/(sici)1097-0134(199705)28:1<10::aid-prot2>3.0.co;2-n" @default.
- W2142958665 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9144787" @default.
- W2142958665 hasPublicationYear "1997" @default.
- W2142958665 type Work @default.
- W2142958665 sameAs 2142958665 @default.
- W2142958665 citedByCount "185" @default.
- W2142958665 countsByYear W21429586652012 @default.
- W2142958665 countsByYear W21429586652013 @default.
- W2142958665 countsByYear W21429586652014 @default.
- W2142958665 countsByYear W21429586652015 @default.
- W2142958665 countsByYear W21429586652016 @default.
- W2142958665 countsByYear W21429586652017 @default.
- W2142958665 countsByYear W21429586652018 @default.
- W2142958665 countsByYear W21429586652019 @default.
- W2142958665 countsByYear W21429586652020 @default.
- W2142958665 countsByYear W21429586652021 @default.
- W2142958665 countsByYear W21429586652022 @default.
- W2142958665 countsByYear W21429586652023 @default.
- W2142958665 crossrefType "journal-article" @default.
- W2142958665 hasAuthorship W2142958665A5063489663 @default.
- W2142958665 hasAuthorship W2142958665A5069990985 @default.
- W2142958665 hasConcept C181199279 @default.
- W2142958665 hasConcept C185592680 @default.
- W2142958665 hasConcept C197957613 @default.
- W2142958665 hasConcept C2776901170 @default.
- W2142958665 hasConcept C2779268744 @default.
- W2142958665 hasConcept C2779728014 @default.
- W2142958665 hasConcept C45566312 @default.
- W2142958665 hasConcept C55493867 @default.
- W2142958665 hasConcept C71240020 @default.
- W2142958665 hasConcept C75520062 @default.
- W2142958665 hasConceptScore W2142958665C181199279 @default.
- W2142958665 hasConceptScore W2142958665C185592680 @default.
- W2142958665 hasConceptScore W2142958665C197957613 @default.
- W2142958665 hasConceptScore W2142958665C2776901170 @default.
- W2142958665 hasConceptScore W2142958665C2779268744 @default.
- W2142958665 hasConceptScore W2142958665C2779728014 @default.
- W2142958665 hasConceptScore W2142958665C45566312 @default.
- W2142958665 hasConceptScore W2142958665C55493867 @default.
- W2142958665 hasConceptScore W2142958665C71240020 @default.
- W2142958665 hasConceptScore W2142958665C75520062 @default.
- W2142958665 hasIssue "1" @default.
- W2142958665 hasLocation W21429586651 @default.
- W2142958665 hasLocation W21429586652 @default.
- W2142958665 hasOpenAccess W2142958665 @default.
- W2142958665 hasPrimaryLocation W21429586651 @default.
- W2142958665 hasRelatedWork W1653909930 @default.
- W2142958665 hasRelatedWork W1970496763 @default.
- W2142958665 hasRelatedWork W1986654865 @default.
- W2142958665 hasRelatedWork W1995695834 @default.
- W2142958665 hasRelatedWork W2049704732 @default.
- W2142958665 hasRelatedWork W2079193504 @default.
- W2142958665 hasRelatedWork W2393553660 @default.
- W2142958665 hasRelatedWork W2596200008 @default.
- W2142958665 hasRelatedWork W3095824004 @default.
- W2142958665 hasRelatedWork W4254900740 @default.
- W2142958665 hasVolume "28" @default.
- W2142958665 isParatext "false" @default.
- W2142958665 isRetracted "false" @default.
- W2142958665 magId "2142958665" @default.
- W2142958665 workType "article" @default.