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- W2143084367 abstract "Supplementation of diet with fermented or lactic acid bacteria-containing food products have shown to reduce serum cholesterol levels in humans and animals, and many findings on hypocholesterolemic effect of these products have been published. In this study, we evaluated the in vitro cholesterol-binding and bile salt-deconjugating abilities as well as acid and bile tolerance of lactic acid bacteria isolated from tempoyak. Tempoyak is an Indonesian and Malaysian traditional fermented food made of durian fruit. Results of this study showed that Enterococcus sp. were the dominant lactic acid bacteria followed by Lactobacillus sp. obtained during spontaneous fermentation of durian into tempoyak. Almost all 44 isolates were tolerant to acid, however, only 3 isolates namely Enterococcus sp. strains UP-9, UP-11 and UP-14 were tolerant to bile. Among these 3 isolates, only Enterococcus sp. UP-11 exhibited cholesterolbinding activity. Enterococcus sp. UP-9 showed relatively higher sodium taurocholatedeconjugating ability followed by strain UP-11, while strain UP-14 could not deconjugate sodium taurocholate. Based on the 16S rRNA gene sequences of Enterococcus sp. retrieved from GenBank and aligned with the taxonomically related bacteria compared with GenBank, Enterococcus sp. UP-9 was identified as Enterococcusgallinarum UP-9, and Enterococcus sp. UP-11 was assigned as Enterococcusfaecalis UP-11." @default.
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- W2143084367 date "2013-01-01" @default.
- W2143084367 modified "2023-09-23" @default.
- W2143084367 title "Bile and acid tolerance of lactic acid bacteria isolated from tempoyak and their probiotic potential." @default.
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