Matches in SemOpenAlex for { <https://semopenalex.org/work/W2143146670> ?p ?o ?g. }
Showing items 1 to 94 of
94
with 100 items per page.
- W2143146670 endingPage "156" @default.
- W2143146670 startingPage "154" @default.
- W2143146670 abstract "Dihydroorotase (DHOase) catalyzes the formation of dihydroorotate in the de novo pyrimidine biosynthetic pathway. The gene encoding the type I DHOase from the hyperthermophilic bacterium Aquifex aeolicus has been cloned in Escherichia coli with a polyhistidine affinity tag appended to the amino-terminal end and sequenced. The recombinant protein was expressed at high levels and could be purified readily in a single step by Ni(2+) affinity chromatography. Both native and selenomethionine-labeled proteins were crystallized using the hanging-drop vapor-diffusion technique. Screens of the purified protein identified several conditions that yielded crystals; however, the best crystals were obtained using 1 M Li(2)SO(4), 10 mM NiCl(2), 100 mM Tris acetate pH 8.5 as the precipitant. Well formed diamond-shaped crystals appeared within 1 d and continued to grow over several weeks to about 0.5 mm in the largest dimension. The crystals diffract to 1.7 A and belong to space group C2, with unit-cell parameters a = 119.8, b = 88.0, c = 55.2 A, beta = 99.0 degrees and a mosaic spread of 0.6 degrees. There is one DHOase monomer in the asymmetric unit." @default.
- W2143146670 created "2016-06-24" @default.
- W2143146670 creator A5003771627 @default.
- W2143146670 creator A5024934066 @default.
- W2143146670 creator A5038332935 @default.
- W2143146670 creator A5051059410 @default.
- W2143146670 creator A5063888924 @default.
- W2143146670 creator A5090112131 @default.
- W2143146670 date "2001-12-21" @default.
- W2143146670 modified "2023-09-26" @default.
- W2143146670 title "Cloning, expression and preliminary X-ray analysis of the dihydroorotase from the hyperthermophilic eubacterium<i>Aquifex aeolicus</i>" @default.
- W2143146670 cites W1515436868 @default.
- W2143146670 cites W1528402370 @default.
- W2143146670 cites W1574809776 @default.
- W2143146670 cites W1580814042 @default.
- W2143146670 cites W1590950221 @default.
- W2143146670 cites W1598900371 @default.
- W2143146670 cites W1627698625 @default.
- W2143146670 cites W1969568921 @default.
- W2143146670 cites W2000133966 @default.
- W2143146670 cites W2003687681 @default.
- W2143146670 cites W2037269365 @default.
- W2143146670 cites W2047465253 @default.
- W2143146670 cites W2049780451 @default.
- W2143146670 cites W2060807817 @default.
- W2143146670 cites W2066459154 @default.
- W2143146670 cites W2070260661 @default.
- W2143146670 cites W2081579973 @default.
- W2143146670 cites W2088664558 @default.
- W2143146670 cites W2097910120 @default.
- W2143146670 cites W2100750044 @default.
- W2143146670 cites W2160216877 @default.
- W2143146670 cites W2411779352 @default.
- W2143146670 cites W31396097 @default.
- W2143146670 cites W2243956032 @default.
- W2143146670 doi "https://doi.org/10.1107/s0907444901017528" @default.
- W2143146670 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11752797" @default.
- W2143146670 hasPublicationYear "2001" @default.
- W2143146670 type Work @default.
- W2143146670 sameAs 2143146670 @default.
- W2143146670 citedByCount "12" @default.
- W2143146670 countsByYear W21431466702013 @default.
- W2143146670 countsByYear W21431466702014 @default.
- W2143146670 countsByYear W21431466702017 @default.
- W2143146670 countsByYear W21431466702019 @default.
- W2143146670 crossrefType "journal-article" @default.
- W2143146670 hasAuthorship W2143146670A5003771627 @default.
- W2143146670 hasAuthorship W2143146670A5024934066 @default.
- W2143146670 hasAuthorship W2143146670A5038332935 @default.
- W2143146670 hasAuthorship W2143146670A5051059410 @default.
- W2143146670 hasAuthorship W2143146670A5063888924 @default.
- W2143146670 hasAuthorship W2143146670A5090112131 @default.
- W2143146670 hasConcept C104317684 @default.
- W2143146670 hasConcept C179247698 @default.
- W2143146670 hasConcept C181199279 @default.
- W2143146670 hasConcept C185592680 @default.
- W2143146670 hasConcept C2777400929 @default.
- W2143146670 hasConcept C40767141 @default.
- W2143146670 hasConcept C547475151 @default.
- W2143146670 hasConcept C55493867 @default.
- W2143146670 hasConcept C8010536 @default.
- W2143146670 hasConcept C86803240 @default.
- W2143146670 hasConceptScore W2143146670C104317684 @default.
- W2143146670 hasConceptScore W2143146670C179247698 @default.
- W2143146670 hasConceptScore W2143146670C181199279 @default.
- W2143146670 hasConceptScore W2143146670C185592680 @default.
- W2143146670 hasConceptScore W2143146670C2777400929 @default.
- W2143146670 hasConceptScore W2143146670C40767141 @default.
- W2143146670 hasConceptScore W2143146670C547475151 @default.
- W2143146670 hasConceptScore W2143146670C55493867 @default.
- W2143146670 hasConceptScore W2143146670C8010536 @default.
- W2143146670 hasConceptScore W2143146670C86803240 @default.
- W2143146670 hasIssue "1" @default.
- W2143146670 hasLocation W21431466701 @default.
- W2143146670 hasLocation W21431466702 @default.
- W2143146670 hasOpenAccess W2143146670 @default.
- W2143146670 hasPrimaryLocation W21431466701 @default.
- W2143146670 hasRelatedWork W1973469481 @default.
- W2143146670 hasRelatedWork W1995783458 @default.
- W2143146670 hasRelatedWork W2016628627 @default.
- W2143146670 hasRelatedWork W2064362510 @default.
- W2143146670 hasRelatedWork W2088551442 @default.
- W2143146670 hasRelatedWork W2160374033 @default.
- W2143146670 hasRelatedWork W2361670818 @default.
- W2143146670 hasRelatedWork W2369132793 @default.
- W2143146670 hasRelatedWork W2374434167 @default.
- W2143146670 hasRelatedWork W2435535387 @default.
- W2143146670 hasVolume "58" @default.
- W2143146670 isParatext "false" @default.
- W2143146670 isRetracted "false" @default.
- W2143146670 magId "2143146670" @default.
- W2143146670 workType "article" @default.