FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2143227132> ?p ?o ?g. }

• W2143227132 endingPage "12506" @default.
• W2143227132 startingPage "12495" @default.
• W2143227132 abstract "Virions of porcine reproductive and respiratory syndrome virus (PRRSV) contain six membrane proteins: the major proteins GP5 and M and the minor proteins GP2a, E, GP3, and GP4. Here, we studied the envelope protein requirements for PRRSV particle formation and infectivity using full-length cDNA clones in which the genes encoding the membrane proteins were disrupted by site-directed mutagenesis. By transfection of RNAs transcribed from these cDNAs into BHK-21 cells and analysis of the culture medium using ultracentrifugation, radioimmunoprecipitation, and real-time reverse transcription-PCR, we observed that the production of viral particles is dependent on both major envelope proteins; no particles were released when either the GP5 or the M protein was absent. In contrast, particle production was not dependent on the minor envelope proteins. Remarkably, in the absence of any one of the latter proteins, the incorporation of all other minor envelope proteins was affected, indicating that these proteins interact with each other and are assembled into virions as a multimeric complex. Independent evidence for such complexes was obtained by coexpression of the minor envelope proteins in BHK-21 cells using a Semliki Forest virus expression system. By analyzing the maturation of their N-linked oligosaccharides, we found that the glycoproteins were each retained in the endoplasmic reticulum unless expressed together, in which case they were collectively transported through the Golgi complex to the plasma membrane and were even detected in the extracellular medium. As the PRRSV particles lacking the minor envelope proteins are not infectious, we hypothesize that the virion surface structures formed by these proteins function in viral entry by mediating receptor binding and/or virus-cell fusion." @default.
• W2143227132 created "2016-06-24" @default.
• W2143227132 creator A5000266713 @default.
• W2143227132 creator A5023848129 @default.
• W2143227132 creator A5025765681 @default.
• W2143227132 creator A5029333373 @default.
• W2143227132 creator A5044261622 @default.
• W2143227132 creator A5044496374 @default.
• W2143227132 date "2005-10-01" @default.
• W2143227132 modified "2023-10-02" @default.
• W2143227132 title "Envelope Protein Requirements for the Assembly of Infectious Virions of Porcine Reproductive and Respiratory Syndrome Virus" @default.
• W2143227132 cites W132455992 @default.
• W2143227132 cites W1537278671 @default.
• W2143227132 cites W1598356970 @default.
• W2143227132 cites W192691191 @default.
• W2143227132 cites W1965587122 @default.
• W2143227132 cites W1967105241 @default.
• W2143227132 cites W1979633272 @default.
• W2143227132 cites W1990458043 @default.
• W2143227132 cites W1993103293 @default.
• W2143227132 cites W200611355 @default.
• W2143227132 cites W2007375742 @default.
• W2143227132 cites W2008633361 @default.
• W2143227132 cites W2015537591 @default.
• W2143227132 cites W2017459838 @default.
• W2143227132 cites W2018996246 @default.
• W2143227132 cites W2021751941 @default.
• W2143227132 cites W2029684179 @default.
• W2143227132 cites W2040491682 @default.
• W2143227132 cites W2044397990 @default.
• W2143227132 cites W2048821306 @default.
• W2143227132 cites W2055047899 @default.
• W2143227132 cites W2058239852 @default.
• W2143227132 cites W2058666916 @default.
• W2143227132 cites W2075267599 @default.
• W2143227132 cites W2080488939 @default.
• W2143227132 cites W2081490150 @default.
• W2143227132 cites W2082901720 @default.
• W2143227132 cites W2089270659 @default.
• W2143227132 cites W2092411293 @default.
• W2143227132 cites W2093110887 @default.
• W2143227132 cites W2097066834 @default.
• W2143227132 cites W2097781409 @default.
• W2143227132 cites W2099469492 @default.
• W2143227132 cites W2103751014 @default.
• W2143227132 cites W2107519953 @default.
• W2143227132 cites W2118717590 @default.
• W2143227132 cites W2123852904 @default.
• W2143227132 cites W2124821252 @default.
• W2143227132 cites W2126326366 @default.
• W2143227132 cites W2128729063 @default.
• W2143227132 cites W2131759306 @default.
• W2143227132 cites W2140516125 @default.
• W2143227132 cites W2147786333 @default.
• W2143227132 cites W2149264591 @default.
• W2143227132 cites W2149442307 @default.
• W2143227132 cites W2156490160 @default.
• W2143227132 cites W2164590227 @default.
• W2143227132 cites W2171125979 @default.
• W2143227132 doi "https://doi.org/10.1128/jvi.79.19.12495-12506.2005" @default.
• W2143227132 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1211556" @default.
• W2143227132 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16160177" @default.
• W2143227132 hasPublicationYear "2005" @default.
• W2143227132 type Work @default.
• W2143227132 sameAs 2143227132 @default.
• W2143227132 citedByCount "192" @default.
• W2143227132 countsByYear W21432271322012 @default.
• W2143227132 countsByYear W21432271322013 @default.
• W2143227132 countsByYear W21432271322014 @default.
• W2143227132 countsByYear W21432271322015 @default.
• W2143227132 countsByYear W21432271322016 @default.
• W2143227132 countsByYear W21432271322017 @default.
• W2143227132 countsByYear W21432271322018 @default.
• W2143227132 countsByYear W21432271322019 @default.
• W2143227132 countsByYear W21432271322020 @default.
• W2143227132 countsByYear W21432271322021 @default.
• W2143227132 countsByYear W21432271322022 @default.
• W2143227132 countsByYear W21432271322023 @default.
• W2143227132 crossrefType "journal-article" @default.
• W2143227132 hasAuthorship W2143227132A5000266713 @default.
• W2143227132 hasAuthorship W2143227132A5023848129 @default.
• W2143227132 hasAuthorship W2143227132A5025765681 @default.
• W2143227132 hasAuthorship W2143227132A5029333373 @default.
• W2143227132 hasAuthorship W2143227132A5044261622 @default.
• W2143227132 hasAuthorship W2143227132A5044496374 @default.
• W2143227132 hasBestOaLocation W21432271321 @default.
• W2143227132 hasConcept C104317684 @default.
• W2143227132 hasConcept C108625454 @default.
• W2143227132 hasConcept C119062480 @default.
• W2143227132 hasConcept C144647389 @default.
• W2143227132 hasConcept C153911025 @default.
• W2143227132 hasConcept C158617107 @default.
• W2143227132 hasConcept C159047783 @default.
• W2143227132 hasConcept C187882448 @default.
• W2143227132 hasConcept C2522874641 @default.
• W2143227132 hasConcept C2779819720 @default.
• W2143227132 hasConcept C2780963514 @default.
• W2143227132 hasConcept C41625074 @default.

• next