Matches in SemOpenAlex for { <https://semopenalex.org/work/W2143246496> ?p ?o ?g. }
- W2143246496 endingPage "1205" @default.
- W2143246496 startingPage "1197" @default.
- W2143246496 abstract "Θ-Toxin (perfringolysin O), a cholesterol-binding toxin, was partially proteolyzed and biotinylated (BCΘ) to eliminate hemolyzing activity and was used as a cytochemical probe. In fixed cells, binding of BCΘ was intense in the plasma membrane, especially at the base of apical microvilli and in lateral processes. The labeling was abolished by pretreatment with filipin, digitonin, or tomatin. When living cultured cells were treated with BCΘ and then with either fluorescein–avidin D or colloidal gold–streptavidin, the labeling in fine dots was distributed on the cell surface without local concentration as long as cells were kept on ice. When the temperature was raised to 37C after treatment, the probe formed discrete large patches and became sequestered to caveolae. Binding of BCΘ alone without the secondary reagents did not cause redistribution even at 37C. Because the plasma membrane maintains integrity even after binding of BCΘ, the probe can be used not only for cytochemical labeling of fixed cells but for pursuing the behavior of crosslinked cholesterol molecules in living cells. By use of this new probe, the present study revealed that crosslinked cholesterol in the plasma membrane is sequestered to caveolae." @default.
- W2143246496 created "2016-06-24" @default.
- W2143246496 creator A5011835411 @default.
- W2143246496 creator A5047991971 @default.
- W2143246496 creator A5060186654 @default.
- W2143246496 creator A5069931207 @default.
- W2143246496 date "1997-09-01" @default.
- W2143246496 modified "2023-10-13" @default.
- W2143246496 title "Crosslinked Plasmalemmal Cholesterol Is Sequestered to Caveolae: Analysis with a New Cytochemical Probe" @default.
- W2143246496 cites W1517168785 @default.
- W2143246496 cites W1973389308 @default.
- W2143246496 cites W1974859445 @default.
- W2143246496 cites W1978261674 @default.
- W2143246496 cites W1979791097 @default.
- W2143246496 cites W1996219643 @default.
- W2143246496 cites W2007753996 @default.
- W2143246496 cites W2019979753 @default.
- W2143246496 cites W2024562547 @default.
- W2143246496 cites W2034332162 @default.
- W2143246496 cites W2040800727 @default.
- W2143246496 cites W2042754461 @default.
- W2143246496 cites W2042787593 @default.
- W2143246496 cites W2044231217 @default.
- W2143246496 cites W2048252914 @default.
- W2143246496 cites W2050465028 @default.
- W2143246496 cites W2051563688 @default.
- W2143246496 cites W2056923102 @default.
- W2143246496 cites W2058802595 @default.
- W2143246496 cites W2062315382 @default.
- W2143246496 cites W2064754325 @default.
- W2143246496 cites W2068418997 @default.
- W2143246496 cites W2069444054 @default.
- W2143246496 cites W2084413039 @default.
- W2143246496 cites W2092164532 @default.
- W2143246496 cites W2102130081 @default.
- W2143246496 cites W2106582081 @default.
- W2143246496 cites W2109923597 @default.
- W2143246496 cites W2112465136 @default.
- W2143246496 cites W2118510702 @default.
- W2143246496 cites W2133388060 @default.
- W2143246496 cites W2139741936 @default.
- W2143246496 cites W2340809933 @default.
- W2143246496 cites W4245394973 @default.
- W2143246496 cites W2090152184 @default.
- W2143246496 doi "https://doi.org/10.1177/002215549704500903" @default.
- W2143246496 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9283607" @default.
- W2143246496 hasPublicationYear "1997" @default.
- W2143246496 type Work @default.
- W2143246496 sameAs 2143246496 @default.
- W2143246496 citedByCount "62" @default.
- W2143246496 countsByYear W21432464962012 @default.
- W2143246496 countsByYear W21432464962013 @default.
- W2143246496 countsByYear W21432464962014 @default.
- W2143246496 countsByYear W21432464962017 @default.
- W2143246496 countsByYear W21432464962019 @default.
- W2143246496 countsByYear W21432464962021 @default.
- W2143246496 countsByYear W21432464962022 @default.
- W2143246496 crossrefType "journal-article" @default.
- W2143246496 hasAuthorship W2143246496A5011835411 @default.
- W2143246496 hasAuthorship W2143246496A5047991971 @default.
- W2143246496 hasAuthorship W2143246496A5060186654 @default.
- W2143246496 hasAuthorship W2143246496A5069931207 @default.
- W2143246496 hasBestOaLocation W21432464961 @default.
- W2143246496 hasConcept C12554922 @default.
- W2143246496 hasConcept C185592680 @default.
- W2143246496 hasConcept C204628709 @default.
- W2143246496 hasConcept C2778163477 @default.
- W2143246496 hasConcept C2778920010 @default.
- W2143246496 hasConcept C41625074 @default.
- W2143246496 hasConcept C49866891 @default.
- W2143246496 hasConcept C55493867 @default.
- W2143246496 hasConcept C86803240 @default.
- W2143246496 hasConcept C95444343 @default.
- W2143246496 hasConceptScore W2143246496C12554922 @default.
- W2143246496 hasConceptScore W2143246496C185592680 @default.
- W2143246496 hasConceptScore W2143246496C204628709 @default.
- W2143246496 hasConceptScore W2143246496C2778163477 @default.
- W2143246496 hasConceptScore W2143246496C2778920010 @default.
- W2143246496 hasConceptScore W2143246496C41625074 @default.
- W2143246496 hasConceptScore W2143246496C49866891 @default.
- W2143246496 hasConceptScore W2143246496C55493867 @default.
- W2143246496 hasConceptScore W2143246496C86803240 @default.
- W2143246496 hasConceptScore W2143246496C95444343 @default.
- W2143246496 hasIssue "9" @default.
- W2143246496 hasLocation W21432464961 @default.
- W2143246496 hasLocation W21432464962 @default.
- W2143246496 hasOpenAccess W2143246496 @default.
- W2143246496 hasPrimaryLocation W21432464961 @default.
- W2143246496 hasRelatedWork W1983735510 @default.
- W2143246496 hasRelatedWork W1995215171 @default.
- W2143246496 hasRelatedWork W2009792148 @default.
- W2143246496 hasRelatedWork W2022806912 @default.
- W2143246496 hasRelatedWork W2072013612 @default.
- W2143246496 hasRelatedWork W2121226628 @default.
- W2143246496 hasRelatedWork W2125569599 @default.
- W2143246496 hasRelatedWork W2145610908 @default.
- W2143246496 hasRelatedWork W2167706977 @default.
- W2143246496 hasRelatedWork W4233779068 @default.