Matches in SemOpenAlex for { <https://semopenalex.org/work/W2143289299> ?p ?o ?g. }
- W2143289299 endingPage "192" @default.
- W2143289299 startingPage "182" @default.
- W2143289299 abstract "We assessed the rate of in vitro polymerase errors at polythymidine [poly(T)] tracts in the mitochondrial DNA (mtDNA) of a heteroderid nematode (Heterodera cajani). The mtDNA of these nematodes contain unusually high numbers of poly(T) tracts, and have previously been suggested to contain biological poly(T) length variation. However, using a cloned molecule, we observed that poly(T) variation was generated in vitro at regions containing more than six consecutive Ts. This artefactual error rate was estimated at 7.3 × 10(-5) indels/poly(T) tract >6 Ts/cycle. This rate was then compared to the rate of poly(T) variation detected after the amplification of a biological sample, in order to estimate the 'biological + artefactual' rate of poly(T) variation. There was no significant difference between the artefactual and the artefactual + biological rates, suggesting that the majority of poly(T) variation in the biological sample was artefactual. We then examined the generation of poly(T) variation in a range of templates with tracts up to 16 Ts long, utilizing a range of Heteroderidae species. We observed that T deletions occurred five times more frequently than insertions, and a trend towards increasing error rates with increasing poly(T) tract length. These findings have significant implications for studies involving genomes with many homopolymer tracts." @default.
- W2143289299 created "2016-06-24" @default.
- W2143289299 creator A5002791586 @default.
- W2143289299 creator A5022710072 @default.
- W2143289299 creator A5031353061 @default.
- W2143289299 creator A5059806638 @default.
- W2143289299 creator A5072860957 @default.
- W2143289299 creator A5089827196 @default.
- W2143289299 date "2010-12-16" @default.
- W2143289299 modified "2023-10-16" @default.
- W2143289299 title "Poly(T) Variation in Heteroderid Nematode Mitochondrial Genomes is Predominantly an Artefact of Amplification" @default.
- W2143289299 cites W1496033005 @default.
- W2143289299 cites W1924980044 @default.
- W2143289299 cites W1931568898 @default.
- W2143289299 cites W1966108249 @default.
- W2143289299 cites W1966179840 @default.
- W2143289299 cites W1976822588 @default.
- W2143289299 cites W1980771135 @default.
- W2143289299 cites W1992975997 @default.
- W2143289299 cites W1994086433 @default.
- W2143289299 cites W1995625340 @default.
- W2143289299 cites W2004177812 @default.
- W2143289299 cites W2006581389 @default.
- W2143289299 cites W2013727620 @default.
- W2143289299 cites W2015702651 @default.
- W2143289299 cites W2017990250 @default.
- W2143289299 cites W2017993878 @default.
- W2143289299 cites W2020317941 @default.
- W2143289299 cites W2021465107 @default.
- W2143289299 cites W2023138117 @default.
- W2143289299 cites W2030261579 @default.
- W2143289299 cites W2039894638 @default.
- W2143289299 cites W2043133777 @default.
- W2143289299 cites W2045882738 @default.
- W2143289299 cites W2047846317 @default.
- W2143289299 cites W2048335347 @default.
- W2143289299 cites W2049321680 @default.
- W2143289299 cites W2053731523 @default.
- W2143289299 cites W2054065154 @default.
- W2143289299 cites W2056635597 @default.
- W2143289299 cites W2066574980 @default.
- W2143289299 cites W2073978077 @default.
- W2143289299 cites W2081858203 @default.
- W2143289299 cites W2085634387 @default.
- W2143289299 cites W2092309784 @default.
- W2143289299 cites W2095005733 @default.
- W2143289299 cites W2096326962 @default.
- W2143289299 cites W2103250001 @default.
- W2143289299 cites W2103903744 @default.
- W2143289299 cites W2106882534 @default.
- W2143289299 cites W2107426021 @default.
- W2143289299 cites W2113948160 @default.
- W2143289299 cites W2115356185 @default.
- W2143289299 cites W2115495932 @default.
- W2143289299 cites W2117771638 @default.
- W2143289299 cites W2118208041 @default.
- W2143289299 cites W2122139577 @default.
- W2143289299 cites W2128692282 @default.
- W2143289299 cites W2136951727 @default.
- W2143289299 cites W2142215487 @default.
- W2143289299 cites W2147925001 @default.
- W2143289299 cites W2150692609 @default.
- W2143289299 cites W2155011204 @default.
- W2143289299 cites W2167252044 @default.
- W2143289299 cites W2169220960 @default.
- W2143289299 cites W2171596181 @default.
- W2143289299 cites W2333766931 @default.
- W2143289299 doi "https://doi.org/10.1007/s00239-010-9414-3" @default.
- W2143289299 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21161202" @default.
- W2143289299 hasPublicationYear "2010" @default.
- W2143289299 type Work @default.
- W2143289299 sameAs 2143289299 @default.
- W2143289299 citedByCount "14" @default.
- W2143289299 countsByYear W21432892992013 @default.
- W2143289299 countsByYear W21432892992014 @default.
- W2143289299 countsByYear W21432892992015 @default.
- W2143289299 countsByYear W21432892992016 @default.
- W2143289299 countsByYear W21432892992017 @default.
- W2143289299 countsByYear W21432892992019 @default.
- W2143289299 countsByYear W21432892992021 @default.
- W2143289299 countsByYear W21432892992023 @default.
- W2143289299 crossrefType "journal-article" @default.
- W2143289299 hasAuthorship W2143289299A5002791586 @default.
- W2143289299 hasAuthorship W2143289299A5022710072 @default.
- W2143289299 hasAuthorship W2143289299A5031353061 @default.
- W2143289299 hasAuthorship W2143289299A5059806638 @default.
- W2143289299 hasAuthorship W2143289299A5072860957 @default.
- W2143289299 hasAuthorship W2143289299A5089827196 @default.
- W2143289299 hasBestOaLocation W21432892992 @default.
- W2143289299 hasConcept C104317684 @default.
- W2143289299 hasConcept C119054055 @default.
- W2143289299 hasConcept C135763542 @default.
- W2143289299 hasConcept C141231307 @default.
- W2143289299 hasConcept C153209595 @default.
- W2143289299 hasConcept C153911025 @default.
- W2143289299 hasConcept C18903297 @default.
- W2143289299 hasConcept C24586158 @default.
- W2143289299 hasConcept C2778830712 @default.