Matches in SemOpenAlex for { <https://semopenalex.org/work/W2143407286> ?p ?o ?g. }
- W2143407286 endingPage "794" @default.
- W2143407286 startingPage "794" @default.
- W2143407286 abstract "Post-translational protein modifications, such as tyrosine phosphorylation, regulate protein-protein interactions (PPIs) critical for signal processing and cellular phenotypes. We extended an established yeast two-hybrid system employing human protein kinases for the analyses of phospho-tyrosine (pY)-dependent PPIs in a direct experimental, large-scale approach. We identified 292 mostly novel pY-dependent PPIs which showed high specificity with respect to kinases and interacting proteins and validated a large fraction in co-immunoprecipitation experiments from mammalian cells. About one-sixth of the interactions are mediated by known linear sequence binding motifs while the majority of pY-PPIs are mediated by other linear epitopes or governed by alternative recognition modes. Network analysis revealed that pY-mediated recognition events are tied to a highly connected protein module dedicated to signaling and cell growth pathways related to cancer. Using binding assays, protein complementation and phenotypic readouts to characterize the pY-dependent interactions of TSPAN2 (tetraspanin 2) and GRB2 or PIK3R3 (p55γ), we exemplarily provide evidence that the two pY-dependent PPIs dictate cellular cancer phenotypes." @default.
- W2143407286 created "2016-06-24" @default.
- W2143407286 creator A5005914773 @default.
- W2143407286 creator A5006831986 @default.
- W2143407286 creator A5011628067 @default.
- W2143407286 creator A5015869995 @default.
- W2143407286 creator A5028962300 @default.
- W2143407286 creator A5039852528 @default.
- W2143407286 creator A5048523755 @default.
- W2143407286 date "2015-03-01" @default.
- W2143407286 modified "2023-10-18" @default.
- W2143407286 title "Phospho‐tyrosine dependent protein–protein interaction network" @default.
- W2143407286 cites W1481361157 @default.
- W2143407286 cites W1639582946 @default.
- W2143407286 cites W1848348663 @default.
- W2143407286 cites W1969228305 @default.
- W2143407286 cites W1969456625 @default.
- W2143407286 cites W1988805210 @default.
- W2143407286 cites W2005042639 @default.
- W2143407286 cites W2009405916 @default.
- W2143407286 cites W2012129117 @default.
- W2143407286 cites W2012223636 @default.
- W2143407286 cites W2017306734 @default.
- W2143407286 cites W2017352502 @default.
- W2143407286 cites W2019283004 @default.
- W2143407286 cites W2022095303 @default.
- W2143407286 cites W2023754289 @default.
- W2143407286 cites W2024377156 @default.
- W2143407286 cites W2029425286 @default.
- W2143407286 cites W2033208312 @default.
- W2143407286 cites W2034341688 @default.
- W2143407286 cites W2037944919 @default.
- W2143407286 cites W2041030715 @default.
- W2143407286 cites W2050067448 @default.
- W2143407286 cites W2050604034 @default.
- W2143407286 cites W2052771249 @default.
- W2143407286 cites W2055953592 @default.
- W2143407286 cites W2057358596 @default.
- W2143407286 cites W2059743653 @default.
- W2143407286 cites W2067305633 @default.
- W2143407286 cites W2067803871 @default.
- W2143407286 cites W2071609712 @default.
- W2143407286 cites W2077223675 @default.
- W2143407286 cites W2079278662 @default.
- W2143407286 cites W2081375228 @default.
- W2143407286 cites W2085293179 @default.
- W2143407286 cites W2087645253 @default.
- W2143407286 cites W2087655021 @default.
- W2143407286 cites W2093486460 @default.
- W2143407286 cites W2100428311 @default.
- W2143407286 cites W2101181377 @default.
- W2143407286 cites W2102140136 @default.
- W2143407286 cites W2105047045 @default.
- W2143407286 cites W2111336920 @default.
- W2143407286 cites W2116573438 @default.
- W2143407286 cites W2119756377 @default.
- W2143407286 cites W2121273108 @default.
- W2143407286 cites W2123527017 @default.
- W2143407286 cites W2136860804 @default.
- W2143407286 cites W2138352001 @default.
- W2143407286 cites W2140349867 @default.
- W2143407286 cites W2140638448 @default.
- W2143407286 cites W2147054306 @default.
- W2143407286 cites W2147566730 @default.
- W2143407286 cites W2149148688 @default.
- W2143407286 cites W2150420891 @default.
- W2143407286 cites W2150582475 @default.
- W2143407286 cites W2157658519 @default.
- W2143407286 cites W2160677068 @default.
- W2143407286 cites W2160892471 @default.
- W2143407286 cites W2164076798 @default.
- W2143407286 cites W2164682544 @default.
- W2143407286 cites W2171419502 @default.
- W2143407286 cites W313406992 @default.
- W2143407286 doi "https://doi.org/10.15252/msb.20145968" @default.
- W2143407286 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4380928" @default.
- W2143407286 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25814554" @default.
- W2143407286 hasPublicationYear "2015" @default.
- W2143407286 type Work @default.
- W2143407286 sameAs 2143407286 @default.
- W2143407286 citedByCount "66" @default.
- W2143407286 countsByYear W21434072862015 @default.
- W2143407286 countsByYear W21434072862016 @default.
- W2143407286 countsByYear W21434072862017 @default.
- W2143407286 countsByYear W21434072862018 @default.
- W2143407286 countsByYear W21434072862019 @default.
- W2143407286 countsByYear W21434072862020 @default.
- W2143407286 countsByYear W21434072862021 @default.
- W2143407286 countsByYear W21434072862022 @default.
- W2143407286 countsByYear W21434072862023 @default.
- W2143407286 crossrefType "journal-article" @default.
- W2143407286 hasAuthorship W2143407286A5005914773 @default.
- W2143407286 hasAuthorship W2143407286A5006831986 @default.
- W2143407286 hasAuthorship W2143407286A5011628067 @default.
- W2143407286 hasAuthorship W2143407286A5015869995 @default.
- W2143407286 hasAuthorship W2143407286A5028962300 @default.
- W2143407286 hasAuthorship W2143407286A5039852528 @default.
- W2143407286 hasAuthorship W2143407286A5048523755 @default.