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- W2143442977 abstract "Mouse kidney ॆ-1,6-GlcNAc-transferase (GNT) is the key enzyme for the synthesis of a glycosphingolipid (Galॆ1–4(Fucα1–3)GlcNAcॆ1–6(Galॆ1–3)GalNAcॆ1–3Galα1–4Galॆ1–4Glcॆ1-ceramide) that contains the LeX trisaccharide epitope at its nonreducing terminus. The expression of this glycolipid in the kidney is polymorphic; it is expressed in BALB/c but not DBA/2 mice; and a single autosomal gene (Gsl5) is responsible for this polymorphism. We report here the cDNA sequence that encodes the kidney GNT of BALB/c mice, which possess a wild-type Gsl5gene. The deduced amino acid sequence exhibits 847 identity to that of human core 2 ॆ-1,6-GlcNAc-transferase, which suggests that kidney GNT is a mouse homologue of human core 2 ॆ-1,6-GlcNAc-transferase. The GNT mRNA is expressed abundantly in the kidney, but was not detected in other BALB/c organs or in the kidneys of DBA/2 mice by Northern blot analysis. In addition, we were able to clone and sequence another homologous cDNA from the submandibular gland. The two sequences differ only in their 5′-untranslated region. The submandibular gland type of cDNA was detected in various organs of DBA/2 mice by reverse transcription-polymerase chain reaction, which indicates that the submandibular gland type is ubiquitous and that its expression is not regulated by the Gsl5 gene. Results obtained using the long accurate polymerase chain reaction method indicate that the GNT gene is ∼45 kilobases long, and the order of the exons from the 5′-end is exon 1 of the kidney type, exon 1 of the ubiquitous type, exon 2, and exon 3. Exons 2 and 3 are present in both transcripts, and the translated region is in exon 3. These data suggest that the expression of GNT is regulated by an alternative splicing mechanism and also probably by tissue-specific enhancers and that Gsl5 regulates the expression of GNT only in the kidney. Mouse kidney ॆ-1,6-GlcNAc-transferase (GNT) is the key enzyme for the synthesis of a glycosphingolipid (Galॆ1–4(Fucα1–3)GlcNAcॆ1–6(Galॆ1–3)GalNAcॆ1–3Galα1–4Galॆ1–4Glcॆ1-ceramide) that contains the LeX trisaccharide epitope at its nonreducing terminus. The expression of this glycolipid in the kidney is polymorphic; it is expressed in BALB/c but not DBA/2 mice; and a single autosomal gene (Gsl5) is responsible for this polymorphism. We report here the cDNA sequence that encodes the kidney GNT of BALB/c mice, which possess a wild-type Gsl5gene. The deduced amino acid sequence exhibits 847 identity to that of human core 2 ॆ-1,6-GlcNAc-transferase, which suggests that kidney GNT is a mouse homologue of human core 2 ॆ-1,6-GlcNAc-transferase. The GNT mRNA is expressed abundantly in the kidney, but was not detected in other BALB/c organs or in the kidneys of DBA/2 mice by Northern blot analysis. In addition, we were able to clone and sequence another homologous cDNA from the submandibular gland. The two sequences differ only in their 5′-untranslated region. The submandibular gland type of cDNA was detected in various organs of DBA/2 mice by reverse transcription-polymerase chain reaction, which indicates that the submandibular gland type is ubiquitous and that its expression is not regulated by the Gsl5 gene. Results obtained using the long accurate polymerase chain reaction method indicate that the GNT gene is ∼45 kilobases long, and the order of the exons from the 5′-end is exon 1 of the kidney type, exon 1 of the ubiquitous type, exon 2, and exon 3. Exons 2 and 3 are present in both transcripts, and the translated region is in exon 3. These data suggest that the expression of GNT is regulated by an alternative splicing mechanism and also probably by tissue-specific enhancers and that Gsl5 regulates the expression of GNT only in the kidney. Carbohydrate chains of cell-surface glycoconjugates play important roles in cell-cell and cell-matrix communication (1Varki A. Glycobiology. 1993; 3: 97-130Crossref PubMed Scopus (4987) Google Scholar). The diversity of the carbohydrate structures provides a basis for cell-specific recognition. The expression of carbohydrate chains is highly regulated and changes during embryogenesis, differentiation, and oncogenic transformation (2Hakomori S. Kannagi R. J. Natl. Cancer Inst. 1983; 71: 231-251PubMed Google Scholar). The regulatory process may be mediated by many different gene products, including glycosyltransferases (3Tsuji S. J. Biochem. (Tokyo). 1996; 120: 1-13Crossref PubMed Scopus (218) Google Scholar, 4Schachter H. Fukuda M. Hindsgaul O. Molecular Glycobiology. IRL Press, Oxford1994: 87-162Google Scholar), transcription factors (5Wang X.C. O'Hanlon T.P. Young R.F. Lau J.T.Y. Glycobiology. 1990; 1: 25-31Crossref PubMed Scopus (80) Google Scholar, 6Svensson E.C. Conley P.B. Paulson J.C. J. Biol. Chem. 1992; 267: 3466-3472Abstract Full Text PDF PubMed Google Scholar, 7Harduin-Lepers A. Shaper J.H. Shaper N.L. J. Biol. Chem. 1993; 268: 14348-14359Abstract Full Text PDF PubMed Google Scholar, 8Shaper N.L. Harduin-Lepers A. Shaper J.H. J. Biol. Chem. 1994; 269: 25165-25171Abstract Full Text PDF PubMed Google Scholar, 9Kitagawa H. Mattei M.-G. Paulson J.C. J. Biol. Chem. 1996; 271: 931-938Abstract Full Text Full Text PDF PubMed Scopus (64) Google Scholar, 10Rajput B. Shaper N.L. Shaper J.H. J. Biol. Chem. 1996; 271: 5131-5142Abstract Full Text Full Text PDF PubMed Scopus (82) Google Scholar), nucleotide sugar transporters (11Abeijon C. Robbins P.W. Hirschberg C.B. Proc. Natl. Acad. Sci. U. S. A. 1996; 93: 5963-5968Crossref PubMed Scopus (110) Google Scholar, 12Eckhardt M. Mühlenhoff M. Bethe A. Gerardy-Schahn R. Proc. Natl. Acad. Sci. U. S. A. 1996; 93: 7572-7576Crossref PubMed Scopus (149) Google Scholar, 13Miura N. Ishida N. Hoshino M. Yamauchi M. Hara T. Ayusawa D. Kawakita M. J. Biochem. (Tokyo). 1996; 120: 236-241Crossref PubMed Scopus (120) Google Scholar, 14Ishida N. Miura N. Yoshioka S. Kawakita M. J. Biochem. (Tokyo). 1996; 120: 1074-1078Crossref PubMed Scopus (92) Google Scholar), kinases and phosphatases that act on transferases (15Gao L. Gu X.B. Yu D.S. Yu R.K. Zeng G. Biochem. Biophys. Res. Commun. 1996; 224: 103-107Crossref PubMed Scopus (13) Google Scholar, 16Gu X. Preuß U. Gu T. Yu R.K. J. Neurochem. 1995; 64: 2295-2302Crossref PubMed Scopus (44) Google Scholar), and other genes. The objective of our studies is to understand the genetic basis and mechanisms that regulate the expression of carbohydrates. We have identified an autosomal mouse gene (Gsl5) that controls the expression of GlcNAcॆ1–6(Galॆ1–3)GalNAcॆ1–3Gb3Cer and its elongated glycolipids by regulating ॆ-1,6-GlcNAc-transferase (GNT) 1The abbreviations used are: GNT, ॆ-1,6-GlcNAc-transferase; core 2 GnT, core 2 ॆ-1,6-GlcNAc-transferase; Gb3Cer, globotriaosylceramide (Galα1–4Galॆ1–4Glcॆ1-ceramide); RT-PCR, reverse transcription-polymerase chain reaction; bp, base pair(s); kb, kilobase(s); RACE, rapid amplification of cDNA ends; nt, nucleotide(s); ORF, open reading frame. activity in the kidney (17Sekine M. Hashimoto Y. Inagaki F. Yamakawa T. Suzuki A. J. Biochem. (Tokyo). 1990; 108: 103-108Crossref PubMed Scopus (15) Google Scholar). DBA/2 mice are not able to express detectable levels of GNT activity or the glycolipids containing GlcNAcॆ1–6(Galॆ1–3)GalNAcॆ1–3Gb3Cer as a core structure because of a defect of the Gsl5 gene. To elucidate the role of Gsl5, the mouse kidney enzyme was purified, and its substrate specificity was characterized (18Sekine M. Hashimoto Y. Suzuki M. Inagaki F. Takio K. Suzuki A. J. Biol. Chem. 1994; 269: 31143-31148Abstract Full Text PDF PubMed Google Scholar). The results indicated that the enzyme uses Galॆ1–3GalNAcα1 derivative as a good substrate, in addition to the actual glycolipid substrate, Galॆ1–3GalNAcॆ1–3Gb3Cer. A partial sequence of the purified enzyme spanning 35 amino acid residues exhibited 837 homology to the reported human core 2 ॆ-1,6-GlcNAc-transferase (core 2 GnT) (19Bierhuizen M.F.A. Fukuda M. Proc. Natl. Acad. Sci. U. S. A. 1992; 89: 9326-9330Crossref PubMed Scopus (279) Google Scholar). Core 2 GnT catalyzes the transfer of ॆ-GlcNAc to the core 1 structure, Galॆ1–3GalNAcα1-Ser/Thr on glycoproteins, and creates the core 2 structure, GlcNAcॆ1–6(Galॆ1–3)GalNAcα1-Ser/Thr. The formation of the core 2 structure is essential for further elongation of carbohydrate chains of O-glycans. It is known that human elongated core 2 structures carry Lea, LeX, sialyl-Lea, and sialyl-LeX epitopes at the terminus of polylactosamine core chains (20Maemura K. Fukuda M. J. Biol. Chem. 1992; 267: 24379-24386Abstract Full Text PDF PubMed Google Scholar) and that the expression of core 2 structures changes in activated human T cells (21Piller F. Piller V. Fox R.I. Fukuda M. J. Biol. Chem. 1988; 263: 15146-15150Abstract Full Text PDF PubMed Google Scholar), human leukemic cells (22Brockhausen I. Kuhns W. Schachter H. Matta K.L. Sutherland D.R. Baker M.A. Cancer Res. 1991; 51: 1257-1263PubMed Google Scholar), and T cells of individuals who have Wiskott-Aldrich syndrome (23Higgins E.A. Siminovitch K.A. Zhuang D.L. Brockhausen I. Dennis J.W. J. Biol. Chem. 1991; 266: 6280-6290Abstract Full Text PDF PubMed Google Scholar). Therefore, core 2 GnT plays a critical role in regulating the expression of O-glycan structures and in cellular physiology. The polymorphism of the Gsl5 gene provides an excellent model to analyze the regulation of core 2 GnT. We report here cDNA cloning of two forms of the mouse core 2 GnT gene and analysis of its genomic organization and chromosomal location. All reagents were molecular biology grade. [α-32P]dCTP and [γ-32P]ATP were purchased from NEN Life Science Products. Total RNA was prepared with ISOGEN reagents (Nippongene Co. Ltd., Tokyo) containing phenol and guanidine isothiocyanate. Male BALB/c and DBA/2 mice (6–8 weeks of age; obtained from SLC (Shizuoka, Japan)) were killed under ether anesthesia. Freshly obtained organs (∼200 mg) were cut into small pieces and homogenized with 1 ml of ISOGEN. After adding 0.2 ml of chloroform and mixing vigorously, tubes were centrifuged at 15,000 rpm for 15 min at 4 °C. The transparent upper phase was transferred to another tube, and 0.5 ml of isopropyl alcohol was added. After standing at room temperature for 5–10 min, the tubes were centrifuged again at 15,000 rpm for 10 min at 4 °C. Precipitates were washed once with 1 ml of 757 ethanol, centrifuged again, and then dried briefly. Total RNAs obtained were dissolved in diethyl pyrocarbonate-treated water. Poly(A)+RNA was selected with an Oligotex dT30 (Takara, Kyoto, Japan) according to the manufacturer's instructions. By reverse transcription-polymerase chain reaction (RT-PCR) with degenerate oligonucleotide primers, which were designed on the basis of the partial amino acid sequences of purified GNT, we obtained a partial cDNA fragment of 494 bp from BALB/c mouse kidney poly(A)+ RNA. Rapid amplification of 5′-cDNA ends (5′-RACE) with a 5′-AmpliFINDER RACE kit (CLONTECH) and 3′-RACE with a 3′-AmpliFINDER RACE kit (CLONTECH) were achieved according to the manufacturer's instruction using 1 ॖg of poly(A)+ RNA as a template. To elucidate the 5′ terminus of RNA, primer extension was performed. A synthetic oligonucleotide primer that was complementary to the sequence from positions 46 to 75 of kidney cDNA was labeled at the 5′-end with [γ-32P]ATP by T4 polynucleotide phosphokinase and purified with a Nick column (Pharmacia Biotech Inc.). The labeled primer was hybridized with 5 ॖg of poly(A)+ RNA from mouse kidney and extended with Superscript II reverse transcriptase (Life Technologies, Inc.) to produce cDNA complementary to the RNA template. The end-labeled product was precipitated with ethanol and resolved on a denaturing 67 polyacrylamide gel. The gel was then dried and subjected to autoradiography. Two ॖg of poly(A)+ RNA from mouse kidney were subjected to electrophoresis on a 17 agarose gel containing 2.2 m formaldehyde after denaturation in a sample buffer containing 507 formamide. After denaturation and neutralization, RNA was transferred to a nylon membrane (Magnagraph, Micron Separations) and cross-linked by a UV cross-linker (Bio-Rad). The membrane was then prehybridized in a solution containing 507 formamide, 5 × SSPE (1 × SSPE = 150 mmNaCl, 10 mm NaH2PO4, and 1.3 mm EDTA, pH 7.4), 5 × Denhardt's solution (1 × Denhardt's solution = 0.027 Ficoll, 0.027 polyvinylpyrrolidone, and 0.027 bovine serum albumin), 0.17 SDS, and 100 ॖg/ml yeast RNA at 42 °C for 2 h. Hybridization was carried out at 42 °C for 16–18 h in the prehybridization solution containing a labeled probe at 1 × 106 cpm/ml. Membranes were washed twice at room temperature in 2 × SSC (1 × SSC = 150 mmNaCl and 15 mm sodium citrate, pH 7.0) and 0.17 SDS and twice at 65 °C in 1 × SSC and 0.17 SDS. Membranes were analyzed by a BAS2000 bioimage analyzer (Fuji Photo Film Co. Ltd., Tokyo). The probe used was a PCR-amplified 494-bp cDNA fragment corresponding to the coding region. It was labeled by the random primer method using [α-32P]dCTP and a Random labeling kit (Version 2, Takara) and purified with a Nick column. First strand cDNA synthesis for RT-PCR was done with a kit (Amersham Corp.) and 5 ॖg of total RNAs from various tissues as templates. First strand cDNAs (250 ng equivalent to RNA for each tissue) were amplified by PCR using sense primers designed on the basis of 5′-end sequences of kidney and submandibular gland cDNAs, a region common to both cDNAs, and an antisense primer designed on the basis of a coding sequence. A BALB/c mouse genomic library was purchased from CLONTECH. The library was grown in Escherichia coli K802 cells at a density of 3 × 104 plaques/plate (9.5 × 13.5 cm). Plaques were transferred to nitrocellulose membranes (BA85, Schleicher & Schull), and 1.8 × 106 plaques were screened with one of three 32P-labeled probes, the 494-bp fragment described above or oligonucleotides complementary to kidney or submandibular gland type-specific sequences. Hybridization was performed at 42 °C overnight, and the membranes were washed with 1 × SSC and 0.17 SDS at 65 °C. They were then exposed to x-ray film for 2 days at −80 °C. Three clones were identified with the 494-bp GNT probe, and one clone each with the oligonucleotide probe for kidney- or submandibular gland-specific fragment sequences. These clones were digested with restriction enzymes and subcloned into the pBluescript II SK− vector. We used an LA-PCR kit (Version 2, Takara) according to the manufacturer's protocol. Genomic DNA (200 ng; as a template), oligonucleotide primers (10 pmol each), and LA Taq DNA polymerase (2.5 units) were contained in a 50-ॖl reaction mixture. Amplification was carried out by one cycle of 94 °C for 1 min, 14 cycles of 98 °C for 25 s and 68 °C for 20 min, and 16 cycles of 98 °C for 25 s, 68 °C for 20–24 min (increasing 15 s/cycle), and 72 °C for 10 min. The reaction mixture was concentrated by ethanol precipitation and then subjected to 0.47 agarose gel electrophoresis (Seakem Gold, FMC Corp. BioProducts). Ten ॖg of genomic DNA were digested with EcoRI, BamHI, SalI,SacI, and HindIII at 37 °C overnight, electrophoresed, blotted to a nylon membrane, and hybridized with the32P-labeled 494-bp probe. DNA sequences were analyzed by the dideoxynucleotide chain termination method (24Sanger F. Nicklen S. Coulson A.R. Proc. Natl. Acad. Sci. U. S. A. 1977; 74: 5463-5467Crossref PubMed Scopus (52674) Google Scholar). Genomic DNA from AKXD recombinant inbred strains of mice was a generous gift from Drs. Y. Maeda and H. Yonekawa (Tokyo Metropolitan Institute of Medical Science) (25Bailey D.W. Foster H.L. Small J.D. Fox J.G. The Mouse in Biological Research. I. Academic Press, New York1981: 223-234Google Scholar). Ten ॖg of genomic DNA from AKXD recombinant inbred strains of mice were digested with EcoRI, electrophoresed, blotted, and hybridized as described above for genomic Southern analysis. We cloned a mouse kidney cDNA of 2047 nucleotides (nt) by using PCR and the 5′- and 3′-RACE methods. As shown in Fig. 1, the amino acid sequence deduced from the cDNA consists of 428 residues with a calculated molecular mass of 49.8 kDa and contains amino acid sequences identical to those of four peptides obtained from the purified enzyme (18Sekine M. Hashimoto Y. Suzuki M. Inagaki F. Takio K. Suzuki A. J. Biol. Chem. 1994; 269: 31143-31148Abstract Full Text PDF PubMed Google Scholar). The amino acid sequence also consists of a short N terminus, a hydrophobic transmembrane portion, and a long catalytic domain, indicating the structure of a typical type 2 membrane protein. There are two possible N-glycosylation sites. The sequence exhibits 847 homology to that of human core 2 GnT, suggesting that kidney GNT is a mouse homologue of core 2 GnT. The cDNA of 2047 nt contains a 5′-untranslated region of 356 nt. Data obtained from the primer extension assay, using kidney mRNA, indicate that the start site is 75 nt upstream from the primer (Fig.2), which confirms the identification of the 5′ terminus of kidney cDNA shown in Fig. 1. Northern blotting of kidney RNA with a 494-bp probe (dashed line in Fig. 1) that is part of the open reading frame (ORF) demonstrates that there are two major transcripts, 5.4 and 2.2 kb, in BALB/c mice (Fig. 3). Although the cloned cDNA contains the 404-nt 3′-untranslated region and polyadenylation signal (AATAAA) at nucleotide 2021, there might be another polyadenylation signal farther downstream. Other tissues, including the submandibular gland, liver, spleen, thymus, lung, and brain of both BALB/c and DBA/2 mice, did not give signals on the Northern blot. It should be noted that no signal was detected in the kidney tissue of DBA/2 mice, which suggests that the Gsl5 gene regulates transcription of the GNT gene or that a mutatedGsl5 gene produces an unstable mRNA. If the core 2 and extended core 2 structures have important biological functions, how do DBA/2 mice compensate for the absence of the enzyme? To answer this question, we looked at cDNAs obtained from the submandibular gland of wild-type mice that express the Gsl5gene, although signals were not detected on the Northern blot with the 494-bp probe. A cDNA was amplified by 5′-RACE from poly(A)+ RNA of the submandibular gland of BALB/c mice. Submandibular gland cDNA is 2024 nt long, which is 23 nt shorter than kidney cDNA. As shown in Fig. 4, the major difference between two sequences is in the length of their 5′-untranslated regions, 80 nt in kidney cDNA and 62 nt in submandibular gland cDNA. Another difference is that the 3′-untranslated region of submandibular gland cDNA is 5 nt shorter than that of kidney cDNA. Aside from these differences, both sequences are identical and have the same ORF. These results suggest the existence of tissue-specific promoters and alternative splicing. Tissue-specific expression of the two transcripts was investigated by RT-PCR with sets of primers that were specific for either kidney or submandibular gland cDNA. As shown in Fig. 5, the kidney type of transcript was highly expressed in BALB/c kidney, but not in DBA/2 kidney, and no signals were detected in other organs of BALB/c and DBA/2 mice. In contrast, the submandibular gland type of cDNA was detected also in the kidney, liver, stomach, spleen, lung, and brain of both mouse strains. The expression of submandibular gland cDNA is similar in DBA/2 and BALB/c kidney, spleen, lung, and brain, but not stomach. These results indicate that the two transcripts are produced by independent transcriptional regulation and that DBA/2 mice have a defect only in the expression of the kidney transcript. We isolated five clones by genomic screening. Three clones (m4, m7, and m20) were identified by a 494-bp probe specific for a part of the ORF. Clone m42 was identified by a probe specific for a kidney 5′-untranslated region, and clone m32 hybridized with a probe specific for a submandibular gland 5′-untranslated region. Restriction mapping indicated that clones m4, m7, and m20 overlap each other and that clones m42 and m32 are unique. We used LA-PCR to determine the order of the 5′-untranslated regions of the kidney (exon 1) and submandibular gland (exon 1′) genes. As shown in Fig. 6 A, LA-PCR was performed with a combination of sense primers for the 5′-flanking sequences specific to the kidney type (1s) and submandibular gland type (1′s), an antisense primer for a common sequence (2a), and other combinations of sense (1s and 1′s) and antisense (1a and 1′a) primers. Fig. 6 B displays the results of the LA-PCR analysis. A 17-kb product was obtained using primers 1s and 1′a, and a 25-kb product using primers 1′s and 2a, but no products were obtained with reverse combinations of these primers. Although we could not obtain the product using primers 1s and 2a, probably because the distance was too large, we concluded that the kidney-type exon 1 is located 17 kb 5′-upstream of the submandibular gland-type exon 1′ and that the common sequence is located 42 kb downstream of exon 1. The genomic DNA from DBA/2 mice, which do not express a kidney-type transcript, gave the same LA-PCR products as that from BALB/c mice (Fig. 6 B). We confirmed that nested PCR with these LA-PCR products as templates gave bands with estimated sizes. These results suggest that a deletion or rearrangement detectable by LA-PCR does not occur in DBA/2 mice. Comparing the sequences of cDNA and genomic DNA, we found that the common sequence was contained in two exons separated by a 2.8-kb intron. The exon/intron boundaries are indicated in the legend of Fig.6 A, and splice-junction sequences are in accord with the GT-AG rule (26Breathnach R. Chambon P. Annu. Rev. Biochem. 1981; 50: 349-383Crossref PubMed Scopus (3297) Google Scholar). Overall, the data indicate that the GNTgene contains at least four exons spanning over 45 kb and that the coding sequence is in exon 3, as shown in Fig. 6 A. Genomic Southern blotting indicates that there is only one copy of the gene per haplotype (data not shown). A restriction fragment length polymorphism analysis was performed usingEcoRI to map the GNT gene. BALB/c and AKR/J mice, both of which express GlcNAcॆ1–6(Galॆ1–3)GalNAcॆ1–3Gb3Cer and its extended glycolipids in the kidney, exhibit a 2.7-kb EcoRI fragment of the GNT gene. DBA/2 mice, which do not express those glycolipids, exhibit a 6.2-kb EcoRI fragment. UsingEcoRI digests, the strain distribution pattern of theGNT gene was analyzed in recombinant inbred strains of AKR and DBA/2 mice (AKXD recombinant inbred strains). TableI demonstrates that the strain distribution pattern of Gsl5 is identical to that ofGNT and that the GNT gene is located between theCD5 and Rln gene loci on mouse chromosome 19. TheGsl5 gene responsible for the expression of the glycolipids and the GNT activity in the kidney was not separated from theGNT gene by the recombinant inbred strains we used. The results described above suggest that the Gsl5 gene might encode for a trans-acting transcription factor or might be the promoter region of the GNT gene.Table IStrain distribution patterns of loci on chromosome 19 in AKXD recombinant inbred strainsLocus/AKXD strain111111111222222222 1236789012345678012345678Ly10DDDAADADAAADDDAAAADAADAAD1-aA, AKR/J type; D, DBA/2 type; -, not tested. × indicates a recombination between loci.Lymphocyte antigen 10× ×CD5DDDAADADADADDAAAAADAADAADCluster determinant 5× × ×Gs15-DDD–A-A-DD-A-ADA-AADAADGlycolipids in kidneyGNTDDDDADA-A-DDDA-ADADAADAADॆ-1,6-GlcNAc-transferase× × ×RlnDDDAADAAADDDDD-ADADAADDADRelaxin1-a A, AKR/J type; D, DBA/2 type; -, not tested. × indicates a recombination between loci. Open table in a new tab We have isolated two cDNAs from the kidney and submandibular gland that encode mouse core 2 ॆ-1,6-GlcNAc-transferase. The two cDNAs have identical ORFs and an untranslated exon 2 and differ only in the first exon, which suggests the utilization of alternative promoters and alternative splicing. The submandibular gland type is expressed in many tissues and is ubiquitous, but its expression was so low that we hardly detected it by Northern blot analysis. The other cDNA is found only in the kidney, where it is expressed abundantly and is apparently regulated by a kidney-specific promoter. DBA/2 mice express the ubiquitous type of mRNA in their kidneys. Using kidney microsomes as a source of enzyme and Galॆ1–3GalNAcॆ1–3Gb3Cer or Galॆ1–3GalNAcα1 derivative as a substrate (18Sekine M. Hashimoto Y. Suzuki M. Inagaki F. Takio K. Suzuki A. J. Biol. Chem. 1994; 269: 31143-31148Abstract Full Text PDF PubMed Google Scholar), we detected a very low level of GNT activity, barely above background. These results indicate that theGsl5 gene is responsible for the expression of high levels of GNT mRNA and protein, in contrast to the low activity of the ubiquitous type of GNT enzyme. Therefore, to characterize the ubiquitous enzyme, further studies are required, including optimization of the conditions for enzyme assay. In addition, we plan to determine whether the activity of the ubiquitous GNT enzyme in DBA/2 kidney is sufficient to produce core 2 and elongated core 2 structures in glycoproteins. The specificity of the enzymes produced by the kidney-type and ubiquitous type mRNAs should be the same because both cDNAs encode the same ORF, unless tissue-specific post-translational modification is involved. The deduced amino acid sequence of GNT shares 847 homology with that of human core 2 ॆ-1,6-GlcNAc-transferase (19Bierhuizen M.F.A. Fukuda M. Proc. Natl. Acad. Sci. U. S. A. 1992; 89: 9326-9330Crossref PubMed Scopus (279) Google Scholar), and the Southern blot indicates that the mouse has only one copy of the GNT gene. We have mapped the mouse GNT gene to chromosome 19, where it is closely linked to the Gsl5 gene. The human core 2 GnT gene was mapped to human chromosome 9q21 (27Bierhuizen M.F.A. Mattei M.-G. Fukuda M. Genes Dev. 1993; 7: 468-478Crossref PubMed Scopus (138) Google Scholar), and the human chromosome segment including 9q21 exhibits synteny with the mouse segment that includes the Gsl5 and GNT genes (28Copeland N.G. Jenkins N.A. Gilbert D.J. Eppig J.T. Maltais L.J. Miller J.C. Dietrich W.F. Weaver A. Lincoln S.E. Steen R.G. Stein L.D. Nadeau J.H. Lander E.S. Science. 1993; 262: 57-66Crossref PubMed Scopus (529) Google Scholar). These results suggest that the cloned GNT is the mouse homologue of human core 2 GnT, although it has not been demonstrated whether human core 2 GnT can take the glycolipid as a substrate or not. The genomic structures are different, however. Tissue-specific splicing occurs in mice, but not in humans, and a pseudogene is present in humans, but not in mice (29Bierhuizen M.A. Maemura K. Fukuda M. Glycoconjugate J. 1995; 12: 857-864Crossref PubMed Scopus (5) Google Scholar). These results suggest that tissue-specific alternative splicing and kidney-specific enhancement of GNT occurred in mice after the divergence of mice and humans. Encoding of the ORF in one exon is conserved in both species, however. Tissue-specific alternative splicing has been reported for several glycosyltransferases, including α-2,3- (9Kitagawa H. Mattei M.-G. Paulson J.C. J. Biol. Chem. 1996; 271: 931-938Abstract Full Text Full Text PDF PubMed Scopus (64) Google Scholar) and α-2,6-sialyltransferases (30Wen D.X. Svensson E.C. Paulson J.C. J. Biol. Chem. 1992; 267: 2512-2518Abstract Full Text PDF PubMed Google Scholar, 31Aasheim H.-C. Aas-Eng D.A. Deggerdal A. Blomhoff H.K. Funderud S. Smeland E.B. Eur. J. Biochem. 1993; 213: 467-475Crossref PubMed Scopus (66) Google Scholar), ॆ-1,4-Gal-transferase (7Harduin-Lepers A. Shaper J.H. Shaper N.L. J. Biol. Chem. 1993; 268: 14348-14359Abstract Full Text PDF PubMed Google Scholar, 8Shaper N.L. Harduin-Lepers A. Shaper J.H. J. Biol. Chem. 1994; 269: 25165-25171Abstract Full Text PDF PubMed Google Scholar), and GlcNAc-transferases I (32Yang J. Bhaumik M. Liu Y. Stanley P. Glycobiology. 1994; 4: 703-712Crossref PubMed Scopus (18) Google Scholar) and V (33Saito H. Gu J. Nishikawa A. Ihara Y. Fujii J. Kohgo Y. Taniguchi N. Eur. J. Biochem. 1995; 233: 18-26Crossref PubMed Scopus (46) Google Scholar). The 5′-flanking regions of the α-2,3-sialyltransferase isoforms contain heterogeneous transcriptional start sites and different transcriptional regulation sequences (9Kitagawa H. Mattei M.-G. Paulson J.C. J. Biol. Chem. 1996; 271: 931-938Abstract Full Text Full Text PDF PubMed Scopus (64) Google Scholar). Shaper and co-workers (10Rajput B. Shaper N.L. Shaper J.H. J. Biol. Chem. 1996; 271: 5131-5142Abstract Full Text Full Text PDF PubMed Scopus (82) Google Scholar) reported that binding sites for Sp1 are present in the 5′-flanking region of the ubiquitous type of ॆ-1,4-galactosyltransferase, and multiple binding sites, including AP2, in the 5′-flanking region of the tissue-specific enzyme. Valletet al. (34Vallet V. Bens M. Antoine B. Levrat F. Miquerol L. Kahn A. Vandewalle A. Exp. Cell Res. 1995; 216: 363-370Crossref PubMed Scopus (25) Google Scholar) reported recently that activation of aldolase B in the proximal tubules of mouse kidney requires 5′-flanking region binding sites for hepatocyte nuclear factor 1α, CCAAT/enhancer-binding protein, and D site-binding protein. Igarashiet al. (35Igarashi P. Whyte D.A. Li K. Nagami G.T. J. Biol. Chem. 1996; 271: 9666-9674Abstract Full Text Full Text PDF PubMed Scopus (98) Google Scholar) also reported that the hepatocyte nuclear factor 1-binding site is necessary for kidney-specific expression of the Na+-K+-Cl− cotransporter gene. Our preliminary sequence analysis suggests that the 5′-flanking regions of the two core 2 GnT transcripts in the BALB/c genome are quite different from each other. The ubiquitous type contains binding sites for Sp1, and the other contains a TATA box, a hepatocyte nuclear factor 1 site, and several other binding sites (data not shown). Analysis of the 5′-upstream sequences of the two BALB/c transcripts and comparison of the BALB/c and DBA/2 sequences are now in progress. With regard to alternative splicing of the GNT gene in mice, another interesting cDNA has been reported by Warren. 2Warren, C. E. (1995) GenBank™ accession number MMU19265. It was cloned from MDAY-Dw33 cells, which are derived from a lymphoma of a DBA/2 mouse. Its 5′-untranslated region (306 bp long) is completely different from that of our two cDNAs, and there are differences in seven nucleotides and two deduced amino acids in the open reading frame. These data indicate that the MDAY cDNA is another alternative splicing product. We searched for this 5′-untranslated segment in our genomic clones and found that a 205-bp segment containing the 3′-end (indicated as E1“-2 in Fig. 6 A) was included in clone m20 and that the rest of the 101-bp segment at the 5′-side (indicated as E1”-1 in Fig. 6 A) was located 5.5 kb upstream from exon 1“-2. Thus, the genomic arrangement of these three transcripts is exon 1–exon 1′–exon 1”-1–exon 1“-2–exon 2–exon 3. This scheme explains how the clone of Warren was derived from a DBA/2 mouse and suggests that there are at least three transcripts produced by alternative splicing and tissue-specific promoters. We tried to determine the transcriptional activity of the 5′-upstream sequence of kidney exon 1, ligated to a luciferase gene, in several cell lines derived from kidney, but we have not succeeded yet. Granovsky et al. (36Granovsky M. Fode C. Warren C.E. Campbell R.M. Marth J.D. Pierce M. Fregien N. Dennis J.W. Glycobiology. 1995; 5: 797-806Crossref PubMed Scopus (36) Google Scholar) reported expression of core 2 GnT in post-implantation mouse embryos. They performed in situ RNA hybridization using a mouse core 2 GnT cDNA reported by Warren2 as a probe. The core 2 GnT message was detected in epithelial cells of most mucin-producing organs, such as kidney proximal tubules, intestinal villi, respiratory epithelium, pancreas, and cartilage. The probe they used detects three alternatively spliced transcripts and is not able to determine which type of transcripts are expressed in these tissues. Further studies are necessary to clarify whether the kidney-type mRNA is expressed in mouse embryos and which transcripts are present in DBA/2 embryos. Understanding the mechanism of regulation of core 2 GnT is essential for studies of functions of core 2 and elongated core 2 carbohydrate chains. It is interesting to consider why alternative promoters are used to produce an identical transferase protein and whether there are any functional consequences of the absence of the kidney type of transferase in DBA/2 kidneys. The polymorphic expression of kidney-specific GNT in BALB/c and DBA/2 mice provides an excellent opportunity to study the regulation and function of this enzyme. We thank Dr. D. M. Marcus (Baylor College of Medicine) for assistance with the manuscript." @default.
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