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- W2143541917 abstract "scaffolds with a diameter of 65 mm and a thickness of 3 mm were used. The PLGA microspheres were produced by solvent evaporation and at a size of about 50 µm. The BMP-2 concentration in the microspheres was about 0.05 µg per mg PLGA. The total amount of BMP2 in the dynamic cultivations was 2.5 µg in each bioreactor. The BMP-2 release and the concentration in the medium was measured with a BMP-2 ELISA (Quantikine, R&D Systems) The BMP-2 concentration in medium samples of bioreactor 4 decreases almost linear from 875 pg/ml to zero pg/ml in the first 16 days of the dynamic cultivation. Cells" @default.
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- W2143541917 date "2011-11-22" @default.
- W2143541917 modified "2023-09-27" @default.
- W2143541917 title "Osteogenic Differentiation of adipose mesenchymal stem cells with BMP-2 embedded microspheres in a rotating bed bioreactor" @default.
- W2143541917 cites W2153949260 @default.
- W2143541917 doi "https://doi.org/10.1186/1753-6561-5-s8-p74" @default.
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