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- W2143567310 abstract "The reliability of lanthanide luminescence measurements, by both flow cytometry and digital microscopy, would be enhanced by the availability of narrowband emitting, UV excited lanthanide calibration beads. 0.5-, 3-, and 5-µm beads containing a luminescent europium-complex are manufactured. The luminescence distribution of the 5-µm beads is measured with a time-delayed light-scatter-gated luminescence flow cytometer to have a 7.0% coefficient of variation (CV) The spacial distribution of the europium-complex in individual beads is determined to be homogeneous by confocal microscopy. Emission peaks are found at 592, 616 (width 9.9 nm), and 685 nm with a PARISS® spectrophotometer. The kinetics of the luminescence bleaching caused by UV irradiation of the 0.5- and 5-µm beads measured under LED excitation with a fluorescence microscope indicate that bleaching does not interfere with their imaging. The luminescence lifetimes in water and air were 340 and 460 µs, respectively. Thus, these 5-µm beads can be used for spectral calibration of microscopes equipped with a spectrograph, as test particles for time-delayed luminescence flow cytometers, and possibly as labels for macromolecules and cells." @default.
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- W2143567310 date "2009-01-01" @default.
- W2143567310 modified "2023-09-22" @default.
- W2143567310 title "Calibration beads containing luminescent lanthanide ion complexes" @default.
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- W2143567310 doi "https://doi.org/10.1117/1.3103646" @default.
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