Matches in SemOpenAlex for { <https://semopenalex.org/work/W2143635008> ?p ?o ?g. }
- W2143635008 endingPage "1674" @default.
- W2143635008 startingPage "1666" @default.
- W2143635008 abstract "An immunochemical and biochemical study was performed to reveal which of the endothelial plasma membrane proteins become glycated during the early phases of diabetes. The blood front of the lung microvascular endothelial plasmalemma was purified by the cationic colloidal silica method from normal and diabetic (streptozotocin-induced) rats and comparatively analyzed by two-dimensional electrophoresis. No major qualitative differences in the general spectrum of endothelial plasmalemmal proteins were recorded between normoglycemic and hyperglycemic animals. By probing with anti-glucitollysine antibodies, we found that at 1 month after the onset of diabetes, several endothelial membrane polypeptides contained glucose covalently linked to their lysyl residues. Ten days of insulin treatment restored euglycemia in the diabetic animals and completely abolished the membrane nonenzymatic glycosylation. All the glycated polypeptides of the endothelial plasma membrane belong to the peripheral type and are associated with its cytoplasmic face (cell cortex). They were solubilized by buffers of high pH and were not detected in the lung cytosolic fraction (100,000 g). By microsequencing, the major proteins labeled by the anti-glucitollysine have been identified as being actin, annexin I, annexin II, the p34 subunit of the Arp2/3 complex, and the Ras suppressor protein-1. Conversely, the intrinsic endothelial membrane proteins do not seem to be affected by hyperglycemia. This defines the internal face of the endothelial plasma membrane, particularly the cortical cytoskeleton, as a preferential target for nonenzymatic glycosylation in diabetes, with possible consequences on the fluidity of the endothelial plasmalemma and impairment of the endothelial mechanotransducing ability." @default.
- W2143635008 created "2016-06-24" @default.
- W2143635008 creator A5032742659 @default.
- W2143635008 creator A5067302577 @default.
- W2143635008 creator A5077761867 @default.
- W2143635008 date "2001-07-01" @default.
- W2143635008 modified "2023-10-02" @default.
- W2143635008 title "Actin and Annexins I and II Are Among the Main Endothelial Plasmalemma-Associated Proteins Forming Early Glucose Adducts in Experimental Diabetes" @default.
- W2143635008 cites W1600871831 @default.
- W2143635008 cites W1760982454 @default.
- W2143635008 cites W1843394108 @default.
- W2143635008 cites W1902029503 @default.
- W2143635008 cites W1932052686 @default.
- W2143635008 cites W1932328598 @default.
- W2143635008 cites W1964748714 @default.
- W2143635008 cites W1967026365 @default.
- W2143635008 cites W1972146455 @default.
- W2143635008 cites W1982021133 @default.
- W2143635008 cites W1983663252 @default.
- W2143635008 cites W1987172957 @default.
- W2143635008 cites W2000782226 @default.
- W2143635008 cites W2005991627 @default.
- W2143635008 cites W2006986579 @default.
- W2143635008 cites W2007804077 @default.
- W2143635008 cites W2011155901 @default.
- W2143635008 cites W2012322454 @default.
- W2143635008 cites W2024657158 @default.
- W2143635008 cites W2029736971 @default.
- W2143635008 cites W2030281938 @default.
- W2143635008 cites W2031560512 @default.
- W2143635008 cites W2035551567 @default.
- W2143635008 cites W2041109111 @default.
- W2143635008 cites W2055043387 @default.
- W2143635008 cites W2055168506 @default.
- W2143635008 cites W2058982872 @default.
- W2143635008 cites W2061207707 @default.
- W2143635008 cites W2063549906 @default.
- W2143635008 cites W2082953465 @default.
- W2143635008 cites W2090790003 @default.
- W2143635008 cites W2098939161 @default.
- W2143635008 cites W2100837269 @default.
- W2143635008 cites W2103467580 @default.
- W2143635008 cites W2127821660 @default.
- W2143635008 cites W2133756857 @default.
- W2143635008 cites W2138200625 @default.
- W2143635008 cites W2149255929 @default.
- W2143635008 cites W2156592815 @default.
- W2143635008 cites W2157815908 @default.
- W2143635008 cites W2172285517 @default.
- W2143635008 cites W2299593373 @default.
- W2143635008 cites W2396071676 @default.
- W2143635008 cites W2399593684 @default.
- W2143635008 cites W4234355201 @default.
- W2143635008 cites W4246853555 @default.
- W2143635008 cites W4253440092 @default.
- W2143635008 cites W991979207 @default.
- W2143635008 doi "https://doi.org/10.2337/diabetes.50.7.1666" @default.
- W2143635008 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11423489" @default.
- W2143635008 hasPublicationYear "2001" @default.
- W2143635008 type Work @default.
- W2143635008 sameAs 2143635008 @default.
- W2143635008 citedByCount "41" @default.
- W2143635008 countsByYear W21436350082012 @default.
- W2143635008 countsByYear W21436350082013 @default.
- W2143635008 countsByYear W21436350082016 @default.
- W2143635008 countsByYear W21436350082017 @default.
- W2143635008 countsByYear W21436350082019 @default.
- W2143635008 countsByYear W21436350082020 @default.
- W2143635008 countsByYear W21436350082021 @default.
- W2143635008 crossrefType "journal-article" @default.
- W2143635008 hasAuthorship W2143635008A5032742659 @default.
- W2143635008 hasAuthorship W2143635008A5067302577 @default.
- W2143635008 hasAuthorship W2143635008A5077761867 @default.
- W2143635008 hasBestOaLocation W21436350081 @default.
- W2143635008 hasConcept C123012128 @default.
- W2143635008 hasConcept C125705527 @default.
- W2143635008 hasConcept C126322002 @default.
- W2143635008 hasConcept C134018914 @default.
- W2143635008 hasConcept C144647389 @default.
- W2143635008 hasConcept C147990577 @default.
- W2143635008 hasConcept C161573976 @default.
- W2143635008 hasConcept C181199279 @default.
- W2143635008 hasConcept C185592680 @default.
- W2143635008 hasConcept C202751555 @default.
- W2143635008 hasConcept C2776992346 @default.
- W2143635008 hasConcept C2777313579 @default.
- W2143635008 hasConcept C2779306644 @default.
- W2143635008 hasConcept C2780972559 @default.
- W2143635008 hasConcept C41625074 @default.
- W2143635008 hasConcept C55493867 @default.
- W2143635008 hasConcept C555293320 @default.
- W2143635008 hasConcept C71924100 @default.
- W2143635008 hasConcept C86803240 @default.
- W2143635008 hasConcept C95444343 @default.
- W2143635008 hasConcept C98539663 @default.
- W2143635008 hasConceptScore W2143635008C123012128 @default.
- W2143635008 hasConceptScore W2143635008C125705527 @default.
- W2143635008 hasConceptScore W2143635008C126322002 @default.