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• W2143670366 abstract "Abstract : The purpose of this proposal is to isolate and identify estrogen-responsive genes in human breast cancer cells using the chromatin immunoprecipitation (ChIP) protocol. Estrogen receptor (ER) -bound DNA has been isolated using ChIP from human breast cancer NCF-7 (T5) cells (ER positive and hormone dependent). Southern blotting analysis shows that ER-DNA contains ER-responsive sequences (ER, PR, P52 and c-myc). PCR analysis demonstrated that ER-DNA contains the p52 gene promoter, which is expressed in ER positive cells. Recent evidence shows that ER and associated coactivators are recruited to the estrogen inducible promoters in a cyclical manner. It is reasonable to believe that ER interaction with DNA is dynamically altered during estrogen induction. ER-bound DNA from MCF-7 (T5) cells treated with estradiol (E2) for 30, 60 and 120 min were isolated and analyzed by POR. A library of ER-bound DNA from 30 min E2 treated MCF-7 (T5) cells has been constructed. ChIP-ER-DNA fragments were cloned, sequenced, and blast searched in GenBank. Approximately 900 positive colonies were collected from this library, and approximately 100 cloned DNA fragments were sequenced. A - 1/2(ERE)-Sp1 sequence has been identified in PACE4 (pro-protein convertase) and EWS (Ewing's sarcoma) genes. Studies to determine whether these genes respond to estrogen is currently being determined." @default.
• W2143670366 created "2016-06-24" @default.
• W2143670366 creator A5048849645 @default.
• W2143670366 date "2001-10-01" @default.
• W2143670366 modified "2023-09-27" @default.
• W2143670366 title "Isolation of Estrogen-Responsive Genes in Human Breast Cancer Cells" @default.
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• W2143670366 doi "https://doi.org/10.21236/ada399358" @default.
• W2143670366 hasPublicationYear "2001" @default.
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